Browse > Article
http://dx.doi.org/10.4489/KJM.2016.44.4.330

Control of Ginseng Damping-off by Streptomyces sp. A75 and A501  

Lee, Sang Yeob (Agricultural Microbiology Division, National Academy of Agricultural Science)
Song, Jaekyeong (Agricultural Microbiology Division, National Academy of Agricultural Science)
Yun, Bong-Sik (Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University)
Park, Kyeong hun (Ginseng Research Division, National Institute of Horticultural and Herbal Science)
Kim, Jeong Jun (Agricultural Microbiology Division, National Academy of Agricultural Science)
Han, Ji Hee (Agricultural Microbiology Division, National Academy of Agricultural Science)
Publication Information
The Korean Journal of Mycology / v.44, no.4, 2016 , pp. 330-337 More about this Journal
Abstract
Streptomyces sp. A75 and A501 inhibited the mycelial growth of pathogenic Rhizoctonia solani and Pythium sp., which cause the ginseng disease known as damping-off. Three methods were evaluated for the control of these pathogens, using a mixture of the culture broths from Streptomyces sp. A75 and A501. The methods tested were seed dipping with 50-fold diluted broth, drenching of soil with 100-fold diluted broth after sowing, and combined seed dipping and drenching. These methods reduced the incidence of ginseng damping-off caused by R. solani by 81.3%, 84.8%, and 32.2% and that caused by Pythium sp. by 51.0%, 52.1%, and 75.3%, respectively. Based on these results, the combination of seed dipping and soil drenching after sowing using a mixture of the culture broths from Streptomyces sp. A75 and A501 effectively reduced the incidence of damping-off in ginseng.
Keywords
Antifungal activity; Control; Damping-off; Ginseng; Streptomyces sp.;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Li YB, Wang Y, Tang JP, Chen D, Wang SL. Neuroprotective effects of ginsenoside Rg1-induced neural stem cell transplantation on hypoxic-ischemic encephalopathy. Neural Regen Res 2015;10:753-9.   DOI
2 Saba E, Jeon BR, Jeong DH, Lee K, Goo YK, Kim SH, Sung CK, Roh SS, Kim SD, Kim HK, et al. Black ginseng extract ameliorates hypercholesterolemia in rats. J Ginseng Res 2016;40:160-8.   DOI
3 Gui QF, Xu ZR, Xu KY, Yang YM. The efficacy of ginsengrelated therapies in type 2 diabetes mellitus: an updated systematic review and meta-analysis. Medicine (Baltimore) 2016;95:e2584.   DOI
4 Jang KJ, Choi SH, Yu GJ, Hong SH, Chung YH, Kim CH, Yoon HM, Kim GY, Kim BW, Choi YH. Anti-inflammatory potential of total saponins derived from the roots of Panax ginseng in lipopolysaccharide-activated RAW 264.7 macrophages. Exp Ther Med 2016;11:1109-15.   DOI
5 Lee DY, Jeong YT, Jeong SC, Lee MK, Min JW, Lee JW, Kim GS, Lee SE, Ahn YS, Kang HC, et al. Melanin biosynthesis inhibition effects of ginsenoside Rb2 isolated from Panax ginseng berry. J Microbiol Biotechnol 2015;25:2011-5.   DOI
6 The Korean Society of Plant Pathology. List of plant diseases in Korea, 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
7 Agrios, GN. Plant pathology. 5th ed. Boston: Elsevier Academic Press; 2005.
8 Cho DH, Cho HS, Shin JS, Park DW. Control of damping-off and ginseng stem fungus gnat of ginseng. In: KT&G Ginseng research report, 2007. p. 28-33.
9 Hong SK. Survey of ginseng cultivation in Southern Korea. So-yeun (Central Monopoly Institute) 1964;6:27-44.
10 Georgakopoulos DG, Fiddaman P, Leifert C, Malathrakis NE. Biological control of cucumber and sugar beet damping-off caused by Pythium ultimum with bacterial and fungal antagonists. J Appl Microbiol 2002;92:1078-86.   DOI
11 Howell CR, Stipanovic RD. Suppression of Pythium ultimuminduced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 1980;70:712-5.   DOI
12 Zamanizadeh HR, Hatami N, Aminaee MM, Rakhshandehroo F. Application of biofungicides in control of damping disease off in greenhouse crops as a possible substitute to synthetic fungicides. Int J Environ Sci Technol (Tehran) 2011;8:129-36.   DOI
13 Kim JH, Choi YH, Kang SJ, Rhee IK, Joo GJ. Biocontrol of vegetables damping-off by Bacillus ehimensis YJ-37. Kor J Life Sci 2002;12:416-22.   DOI
14 Joo GJ, Kim JH, Kang SJ. Isolation and antifungal activity of Bacillus ehimensis YJ-37 as antagonistic against vegetables damping-off fungi. Kor J Life Sci 2002;12:200-7.   DOI
15 Jo EJ, Kang BG, Jang KS, Choi YH, Kim JC, Choi GJ. Control efficacy of serenade formulation against Rhizoctonia and Pythium damping-off diseases. Res Plant Dis 2014;20:201-5.   DOI
16 Woo EE, Lee GS, Lee IK, Choi JE, Yun BS. Control of ginseng damping-off by Streptomyces sp. A3265. Kor J Mycol 2016;44:193-5.
17 Lahdenpera ML. Streptomyces-a new tool for controlling plant diseases. Agro Food Ind Hi Tech 1991;2:25-7.
18 Elson MK, Kelly JF, Nair MG. Influence of antifungal compounds from a soil-borne actinomycete on Fusarium spp. in asparagus. J Chem Ecol 1994; 20:2835-46.   DOI
19 Handelsman J, Stabb EV. Biocontrol of soilborne plant pathogens. Plant Cell 1996;8:1855-69.   DOI
20 Song J, Lee SC, Kang JW, Baek HJ, Suh JW. Phylogenetic analysis of Streptomyces spp. isolated from potato scab lesions in Korea on the basis of 16S rRNA gene and 16S-23S rDNA internally transcribed spacer sequences. Int J Syst Evol Microbiol 2004;54:203-9.   DOI
21 Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716-21.   DOI
22 Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731-9.   DOI
23 Saintpierre D, Amir H, Pineau R, Sembiring L, Goodfellow M. Streptomyces yatensis sp. nov., a novel bioactive streptomycete isolated from a New-Caledonian ultramafic soil. Antonie Van Leeuwenhoek 2003;83:21-6.   DOI
24 Esnard J, Potter TL, Zuckerman BM. Streptomyces costaricanus sp. nov., isolated from nematode-suppressive soil. Int J Syst Bacteriol 1995;45:775-9.   DOI