Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Airborne Bacteria Concentration and Species Identification in Residential Living Spaces

주택내 주거공간에 따른 부유세균 농도 분포 및 종 동정 연구

  • Kim, Sung-Yeon (Indoor Environment and Noise Research Division, National Institute of Environmental Research) ;
  • Jheong, Weonhwa (Environmental Health Research Division, National Institute of Environmental Research) ;
  • Hwang, Eun-Seol (Indoor Environment and Noise Research Division, National Institute of Environmental Research) ;
  • Kim, Ji-Hye (Water Supply & Sewerage Research Division, National Institute of Environmental Research) ;
  • Jung, Joon-Sig (Indoor Environment and Noise Research Division, National Institute of Environmental Research) ;
  • Lee, Jae-won (Indoor Environment and Noise Research Division, National Institute of Environmental Research) ;
  • Chung, Hyen-Mi (Indoor Environment and Noise Research Division, National Institute of Environmental Research) ;
  • Kwon, Myunghee (Indoor Environment and Noise Research Division, National Institute of Environmental Research)
  • 김성연 (국립환경과학원 환경기반연구부 생활환경연구과) ;
  • 정원화 (환경건강연구부 환경보건연구과) ;
  • 황은설 (국립환경과학원 환경기반연구부 생활환경연구과) ;
  • 김지혜 (환경기반연구부 상하수도연구과) ;
  • 정준식 (국립환경과학원 환경기반연구부 생활환경연구과) ;
  • 이재원 (국립환경과학원 환경기반연구부 생활환경연구과) ;
  • 정현미 (국립환경과학원 환경기반연구부 생활환경연구과) ;
  • 권명희 (국립환경과학원 환경기반연구부 생활환경연구과)
  • Received : 2016.11.18
  • Accepted : 2016.12.19
  • Published : 2016.12.30
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Abstract

Objectives: Exposure to airborne bacteria is associated with adverse health effects such as respiratory and infectious diseases. This study evaluated airborne bacterial concentrations in the living rooms, kitchens, and toilets of 30 homes. Methods: Bacteria were sampled with an MAS100 impactor in three spaces in the subject homes between April 2014 and February 2015. Bacteria were grown on TSA plates for 48 hours at $35^{\circ}C$. The bacterial strains were isolated and amplified by polymerase chain reaction. Results: The most culturable bacteria were found in toilets ($624.0CFU/m^3$, GM: $417.3CFU/m^3$), followed by in the kitchen ($503.8CFU/m^3$, GM: $324.9CFU/m^3$). The dominant genera identified were: Staphylococcus sp.(19%), Micrococcus sp.(16%), and Bacillus sp.(11%) in the indoor air and Bacillus sp. (30%) in the outdoor air. Gram-positive bacteria comprised more than half of all colonies. Conclusion: In this study, culturable bacteria concentrations were higher than those reported in other spaces. Therefore, it is important to control relative humidity and remove moisture to prevent bacteria from multiplying. Additionally, the dominant species in indoor air were Staphylococcus sp. and Micrococcus sp. These are found on the human skin, mucous membranes, and hair, so human activity can affect bacterial distribution. Therefore, cleaning and controlling moisture are important for reducing indoor bacterial concentrations.

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