Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Enhanced Production of Cellulase-Free Thermoactive Xylanase Using Corncob by a Black Yeast, Aureobasidium pullulans CBS 135684

  • Bankeeree, Wichanee (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University) ;
  • Lotrakul, Pongtharin (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University) ;
  • Prasongsuk, Sehanat (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University) ;
  • Kim, Seung Wook (Department of Chemical and Biological Engineering, Korea University) ;
  • Punnapayak, Hunsa (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University)
  • Received : 2016.04.26
  • Accepted : 2016.08.17
  • Published : 2016.12.01
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Abstract

Our aim was to optimize the production of cellulase-free thermoactive xylanase by Aureobasidium pullulans CBS 135684 with statistical methodology based on experimental designs. Among eleven variables, the nutrient sources that had significant effect on xylanase production were corncob, $(NH_4)_2SO_4$, xylose, $KH_2PO_4$ and tween 80, identified by the initial screening method of Plackett-Burman. The optimum concentrations of these five components were subsequently investigated using response surface methodology. The optimal concentrations ($g{\cdot}l^{-1}$) for maximum production of xylanase were corncob, 39.0; $(NH_4)_2SO_4$, 3.0; xylose, 1.8; $KH_2PO_4$ 1.4; and tween 80, 1.4, respectively. An improved xylanase yield of $8.74{\pm}0.84U{\cdot}ml^{-1}$ was obtained with optimized medium which is 2.1-fold higher production than previously obtained results ($4.10{\pm}0.10U{\cdot}ml^{-1}$) after 48 h of cultivation. In addition, the xylanase production under optimal condition reached $10.09{\pm}0.27U{\cdot}ml^{-1}$ after 72 h of cultivation.

Keywords

References

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Cited by

  1. Enzymatic Hydrolysis of Black Liquor Xylan by a Novel Xylose-Tolerant, Thermostable β-Xylosidase from a Tropical Strain of Aureobasidium pullulans CBS 135684 pp.1559-0291, 2018, https://doi.org/10.1007/s12010-017-2598-x
  2. The current status of Aureobasidium pullulans in biotechnology pp.1874-9356, 2017, https://doi.org/10.1007/s12223-017-0561-4