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http://dx.doi.org/10.9713/kcer.2016.54.6.822

Enhanced Production of Cellulase-Free Thermoactive Xylanase Using Corncob by a Black Yeast, Aureobasidium pullulans CBS 135684  

Bankeeree, Wichanee (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University)
Lotrakul, Pongtharin (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University)
Prasongsuk, Sehanat (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University)
Kim, Seung Wook (Department of Chemical and Biological Engineering, Korea University)
Punnapayak, Hunsa (Plant Biomass Utilization Research Unit, Department of Botany, Faculty of Science, Chulalongkorn University)
Publication Information
Korean Chemical Engineering Research / v.54, no.6, 2016 , pp. 822-829 More about this Journal
Abstract
Our aim was to optimize the production of cellulase-free thermoactive xylanase by Aureobasidium pullulans CBS 135684 with statistical methodology based on experimental designs. Among eleven variables, the nutrient sources that had significant effect on xylanase production were corncob, $(NH_4)_2SO_4$, xylose, $KH_2PO_4$ and tween 80, identified by the initial screening method of Plackett-Burman. The optimum concentrations of these five components were subsequently investigated using response surface methodology. The optimal concentrations ($g{\cdot}l^{-1}$) for maximum production of xylanase were corncob, 39.0; $(NH_4)_2SO_4$, 3.0; xylose, 1.8; $KH_2PO_4$ 1.4; and tween 80, 1.4, respectively. An improved xylanase yield of $8.74{\pm}0.84U{\cdot}ml^{-1}$ was obtained with optimized medium which is 2.1-fold higher production than previously obtained results ($4.10{\pm}0.10U{\cdot}ml^{-1}$) after 48 h of cultivation. In addition, the xylanase production under optimal condition reached $10.09{\pm}0.27U{\cdot}ml^{-1}$ after 72 h of cultivation.
Keywords
Endoxylanase; Thermo-tolerant; Black yeast; Corncob; RSM;
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Times Cited By KSCI : 2  (Citation Analysis)
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