Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Epidemiological Cut-off Values Generated for Disc Diffusion Data from Photobacterium damselae

Photobacterium damselae의 디스크 확산법 결과에 대한 Epidemiological Cut-off Value의 결정

  • Kwon, Mun-Gyeong (Aquatic Life Disease Control Division, National Institute of Fisheries Science) ;
  • Lim, Yun-Jin (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Myoung-Sug (Pathology Division, National Institute of Fisheries Science) ;
  • Seo, Jung-Soo (Aquatic Life Disease Control Division, National Institute of Fisheries Science) ;
  • Kim, Do-Hyung (Department of Aquatic Life Medicine, Pukyong National University)
  • 권문경 (국립수산과학원 방역과) ;
  • 임윤진 (부경대학교 수산생명의학과) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 서정수 (국립수산과학원 방역과) ;
  • 김도형 (부경대학교 수산생명의학과)
  • Received : 2016.10.05
  • Accepted : 2016.11.15
  • Published : 2016.12.31
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Abstract

In this study, epidemiological cut-off values were estimated for 44 Photobacterium damselae isolates, since clinical breakpoints have not been established for this pathogenic bacterium. The susceptibility of the isolates to 10 antibiotics was evaluated using internationally standardized disc diffusion protocols. Normalized resistance interpretation was used to generate statistically valid epidemiological cut-off values for the susceptibility data. There were not enough strains exhibiting full sensitivity to ampicillin and amoxicillin to allow analysis of these antibiotics. Because there were only a marginally sufficient number of strains exhibiting full sensitivity to oxytetracycline, the cut-off value generated provided only a provisional estimate. The valid wild-type cut-off values were <13, 13, 9, 22, 25, 27, and 28 mm for gentamicin, cephalexin, erythromycin, oxolinic acid, flumequine, florfenicol, and sulfamethoxazole/trimethoprim, respectively. The application of these cut-off values should greatly facilitate the rational selection of antibiotics for use in commercial fish farms.

Keywords

References

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