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http://dx.doi.org/10.5657/KFAS.2016.0838

Epidemiological Cut-off Values Generated for Disc Diffusion Data from Photobacterium damselae  

Kwon, Mun-Gyeong (Aquatic Life Disease Control Division, National Institute of Fisheries Science)
Lim, Yun-Jin (Department of Aquatic Life Medicine, Pukyong National University)
Kim, Myoung-Sug (Pathology Division, National Institute of Fisheries Science)
Seo, Jung-Soo (Aquatic Life Disease Control Division, National Institute of Fisheries Science)
Kim, Do-Hyung (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.49, no.6, 2016 , pp. 838-844 More about this Journal
Abstract
In this study, epidemiological cut-off values were estimated for 44 Photobacterium damselae isolates, since clinical breakpoints have not been established for this pathogenic bacterium. The susceptibility of the isolates to 10 antibiotics was evaluated using internationally standardized disc diffusion protocols. Normalized resistance interpretation was used to generate statistically valid epidemiological cut-off values for the susceptibility data. There were not enough strains exhibiting full sensitivity to ampicillin and amoxicillin to allow analysis of these antibiotics. Because there were only a marginally sufficient number of strains exhibiting full sensitivity to oxytetracycline, the cut-off value generated provided only a provisional estimate. The valid wild-type cut-off values were <13, 13, 9, 22, 25, 27, and 28 mm for gentamicin, cephalexin, erythromycin, oxolinic acid, flumequine, florfenicol, and sulfamethoxazole/trimethoprim, respectively. The application of these cut-off values should greatly facilitate the rational selection of antibiotics for use in commercial fish farms.
Keywords
Photobacterium damselae; Antibiotic susceptibility; Disc diffusion; Epidemiological cut-off values; Antibiotic therapy;
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1 Ruane NM, Douglasa I, Gearyb M, Carrolla C, Fleminga GTA and Smith P. 2007. Application of normalised resistance interpretation to disc diffusion data on the susceptibility of Aeromonas salmonicida to three quinolone agents. Aquaculture 272, 156-167. http://dx.doi.org/10.1016/j.aquaculture.2007.08.037.   DOI
2 Smith P, Ruane NM, Douglas I, Carroll C, Kronvall G and Fleming GTA. 2007. Impact of inter-lab variation on the estimation of epidemiological cut-off values for disc diffusion susceptibility test data for Aeromonas salmonicida. Aquaculture 272, 168-179. http://dx.doi.org/10.1016/j.aquaculture.2007.07.216   DOI
3 Smith P. 2008. Antimicrobial resistance in aquaculture, Changing trends in managing aquatic animal disease emergencies. In: E-M. Bernoth, ed. Review Scientifique et Technique Office International des Epizooties 27, 243-264.
4 Smith P. 2012. Antimicrobial resistance: complexities and difficulties of determination. In: Improving biosecurity through prudent and responsible use of veterinary medicines in aquatic food production. Bondad-Reantaso MG, Arthur JR and Subasinghe RP, eds. FAO Fisheries and Aquaculture Technical Paper, Rome, 99-118, 207.
5 Smith P and Kronvall G. 2014. Effect of incubation temperature and time on the precision of data generated by antibiotic disc diffusion assays. J Fish Dis 38, 629-636. http://dx.doi.org/10.1111/jfd.12278   DOI
6 Thyssen A and Ollevier F. 2001. In vitro antimicrobial susceptibility of Photobacterium damselae subsp. Piscicida to 15 different antimicrobial agents. Aquaculture 200, 259-269. http://dx.doi.org/10.1016/s0044-8486(01)00517-8   DOI
7 Chiu TH, Kao LY and Chen ML. 2013. Antibiotic resistance and molecular typing of Photobacterium damselae subsp. damselae, isolated from seafood. J Appl Microbiol 114, 1184-1192. http://dx.doi.org/10.1111/jam.12104   DOI
8 Yasunaga N and Yasumoto S. 1988. Therapeutic effect of florfenicol on experimentally induced pseudotuberculosis in yellowtail. Fish Pathol 23, 1-5. http://dx.doi.org/10.3147/jsfp.23.1   DOI
9 Avendano-Herreraa R, Molina A, Magarinosc B, Toranzoc AE and Smith P. 2011. Estimation of epidemiological cut-off values for disk diffusion susceptibility test data for Streptococcus phocae. Aquaculture 314, 44-48. http://dx.doi.org/10.1016/j.aquaculture.2011.01.049   DOI
10 Cho YA, Han HJ, Mun HE, Jung SH, Park MA and Kim JW. 2013. Characterization of Photobacterium damselae subsp. piscicida isolated from cultured starry flounder, Platichthys stellatus in Korea. J Fish Pathol 26, 77-88. http://dx.doi.org/10.7847/jfp.2013.26.2.077   DOI
11 CLSI. 2006. Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated From Aquatic Animals; Approved Guideline. CLSI document M42-A. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, U.S.A.
12 EUCAST (European Committee on Antimicrobial Susceptibility Testing) web site. 2016a. Definition of clinical breakpoints and epidemiological cut off values. Available at: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/EUCAST_SOPs/EUCAST_definitions_of_clinical_breakpoints_and_ECOFFs.pdf Accessed September 20, 2016.
13 CLSI. 2010. Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement. CLSI document M100-S20. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, U.S.A.
14 CLSI. 2011. Generation, presentation and application of antimicrobial susceptibility test data for bacteria of animal origin: a report. Clinical and laboratory standards institute document VET05-R. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, U.S.A.
15 CLSI. 2014. Performance standards for antimicrobial susceptibility testing of bacteria isolated from aquatic animals; Second informational supplement. CLSI document VET03/VET04-S2. Clinical and Laboratory Standards Institute, Wayne, Pennsylvania, U.S.A.
16 EUCAST (European Committee on Antimicrobial Susceptibility Testing) web site. 2016b. EUCAST Disk Diffusion Test Reading guide, Version 4.0. Retrieved from http://www.eucast.org/ast_of_bacteria/disk_diffusion_methodology/ Accessed September 20, 2016.
17 Kawanishi M, Kijima M, Kojima A, Ishihara K, Esaki H, Yagyu K, Takahashi T, Suzuki S and Tamura Y. 2006. Drug resistance and random amplified polymorphic DNA analysis of Photobacterium damselae ssp. piscicida isolates from cultured Seriola (yellowtail, amberjack and kingfish) in Japan. Lett Appl Micorbiol 42, 648-653. http://dx.doi.org/10.1111/j.1472-765x.2005.01820.x.   DOI
18 Kronvall G. 2003. Determination of the real standard distribution of susceptible strains in zone histograms. Internat J Antimic Agent 22, 7-13. http://dx.doi.org/10.1016/s0924-8579(03)00107-9.   DOI
19 Labella A, Berbel C, Manchado M, Castro D and Borrego JJ. 2011. Photobacterium damselae subsp. damselae, an emerging pathogen affecting new cultured marine fish species in southern Spain. In: Recent Advances in Fish Farms. Aral F and Dogu Z, eds. InTech, New York, U.S.A., 135-152.
20 Kwon MG, Park SU, Bang JD and Park SI. 2005. Isolation of pathogenic Photobacterium damselae subsp. damselae from olive flounder, Paralichthys olivaceus. J Fish Pathol 18, 205-214.
21 Lim YJ, Kim DH, Roh HJ, Park MA, Park CI and Smith P. 2016. Epidemiological cut-off values for disc diffusion data generated by standard test protocols from Edwardsiella tarda and Vibrio harveyi. Aquacult Int 24, 1153-1161. http://dx.doi.org/10.1007/s10499-016-9977-0.   DOI
22 Miller A and Reimschuessel R. 2006. Epidemiological cut-off values for antimicrobial agents against Aeromonas salmonicida isolates determined by frequency distributions of minimal inhibitory concentration and diameter of zone of inhibition data. American J Veterinary Res 67, 1837-1843. http://dx.doi.org/10.2460/ajvr.67.11.1837.   DOI
23 Nonaka L, Maruyama F, Miyamoto M, Miyakoshi M, Kurokawa K and Masuda M. 2012. Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment. Microbes Environ 27, 263-272. http://dx.doi.org/10.1264/jsme2.me11338.   DOI