Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification and Biochemical Characterization of a New Xylan-degrading Streptomyces atrovirens Subspecies WJ-2 Isolated from Soil of Jeju Island in Korea

제주도 토양으로부터 자일란 분해 Streptomyces atrovirens subspecies WJ-2 동정 및 효소의 생화학적 특성 규명

  • Kim, Da Som (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Bae, Chang Hwan (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Yeo, Joo Hong (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Chi, Won-Jae (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources)
  • 김다솜 (국립생물자원관 생물자원활용부 유용자원분석과) ;
  • 배창환 (국립생물자원관 생물자원활용부 유용자원분석과) ;
  • 여주홍 (국립생물자원관 생물자원활용부 유용자원분석과) ;
  • 지원재 (국립생물자원관 생물자원활용부 유용자원분석과)
  • Received : 2016.09.12
  • Accepted : 2016.10.17
  • Published : 2016.12.28
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Abstract

A bacterial strain was isolated from a soil sample collected on Jeju Island, Korea. The strain, designated WJ-2, exhibited a high xylanase activity, whereas cellulase activity was not detected. The 16S rRNA gene sequence of WJ-2 was highly similar to type strains of the genus Streptomyces. A neighbor-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain WJ-2 is phylogenetically related to Streptomyces atrovirens. Furthermore, DNA-DNA hybridization analysis confirmed that strain WJ-2 is a novel subspecies of Streptomyces atrovirens. The genomic DNA G+C content was 73.98 mol% and the major fatty acid present was anteiso-C15:0 (36.19%). The growth and xylanase production of strain WJ-2 were significantly enhanced by using soytone and xylan as nitrogen and carbon sources, respectively. Crude enzyme preparations from the culture broth of strain WJ-2 exhibited maximal total xylanase activities at pH 7.0 and $55^{\circ}C$. Thin-layer chromatography analysis revealed that the crude enzyme degrades beechwood xylan to yield xylobiose and xylotriose as the principal hydrolyzed end products.

제주도에서 채집된 토양시료로부터 xylanase 활성을 나타내는 균주를 분리하여 WJ-2로 명명하였다. 균주 WJ-2의 16S rRNA 유전자 염기서열을 결정하여 이를 토대로 상동성을 검색한 결과, Streptomyces 속의 균주들과 높은 염기서열 상동성을 보였다. 16S rRNA 유전자 염기서열을 토대로하는 neighbor-joining 계통수를 제작하여 Streptomyces atrovirens와 가장 높은 계통발생적 연관성이 갖고 있는 것을 밝혔다. 또한 DNA-DNA hybridization 분석을 통하여 Streptomyces atrovirens의 신규한 아종임을 증명하였다. 균주 WJ-의 게놈내 GC 농도는 73.98 mol%이었으며, 주요 세포벽 지방산으로 anteiso-$C_{15:0}$ (36.19%)을 함유하고 있었다. 균주 WJ-2의 성장 및 xylanase 생산은 배지내에 질소원으로 soytone과 탄소원으로 xylan을 첨가하였을 때 급격히 증가되는 것을 확인하였다. 액체배양액으로부터 준비된 조효소의 xylanase 활성은 pH 7.0과 $55^{\circ}C$에서 가장 높게 나타났다. Thin layer chromatography (TLC) 분석을 통하여 균주 WJ-2의 조효소는 xylan을 분해하여 최종분해산물로서 xylobiose와 xylotriose 생산하는 효소임을 확인하였다.

Keywords

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