Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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A Review of the Characteristics of Early Apparatus and Methods for Hemoglobin Estimation

Hemoglobin 평가를 위한 초기 기구의 특성 및 측정법 고찰

  • Kwon, Young-Il (Department of Biomedical Laboratory Science, Shinhan University)
  • 권영일 (신한대학교 임상병리학과)
  • Received : 2016.11.03
  • Accepted : 2016.11.20
  • Published : 2016.12.31
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Abstract

Since the late 19th century, scientific logic and techniques have been used extensively in the field of clinical pathology, including many laboratory tests utilizing various apparatuses and instruments. Among the techniques to measure hemoglobin, the visual color comparison method was most popular around this time; the specific gravity method and gasometric method were not widely adopted. Instruments that use the visual color comparison method include Gowers' hemoglobinometer, von Fleischl's hemoglobinometer, Dare's hemoglobinometer, Oliver's hemoglobinometer, Haden-Hausser hemoglobinometer, and Spencer Hb meter. Initially, the visual color comparison methods were used to diluate and hemolyze blood with distilled water and then to measure its color. Later, these methods were further developed to measure hemoglobin without dilution, and improved with the formation of acid or alkaline hematin ensuring the stability of color development. Hammerschlag's method as well as the Schmaltz and Peiper's methods were based on specific gravity measurement, but they were not widely used. The gasometric method used the Van Slyke gasometer, indirectly measuring the hemoglobin concentration. This method provides the most accurate results. This survey examined the characteristics and limitations of hemoglobinometers and methods used to measure hemoglobin from the late 19th century to the early-and mid-20th century. Moreover, this study aims to improve the understanding and applicability of the current methods and emerging technologies used in measuring hemoglobin. It is also expected that this investigation is the starting point to promote awareness of the need to organize historical data for a variety of historical relics of the diagnostic laboratory tests.

19세기 후반부터 과학적 논리와 기술이 임상병리학 분야에 적극적으로 활용되기 시작하였다. 이로 인해 많은 검사들이 도구와 기기를 이용하기 시작하였다. 이 때 등장한 혈색소 측정법 중에서 육안색조비교법이 일반적으로 사용되었고, 비중측정법과 가스정량법은 활용도가 낮았다. 육안색조비교법을 적용한 기기로는 Gowers' hemoglobinometer, von Fleischl's hemoglobinometer, Dare's hemoglobinometer, Oliver's hemoglobinometer 그리고 Haden-Hausser hemoglobinometer, Spencer Hb meter 등이 있다. 육안색조비색법은 초기에 혈액을 증류수를 이용하여 희석하고 용혈시킨 후 색조를 측정하는 방법을 활용하였지만, 이후 측정방법을 개선하면서 혈액을 희석없이 측정하거나 발색의 안전성을 위해 acid 혹은 alkaline hematin을 형성시키는 방법들로 발전하였다. 비중을 이용한 측정법으로 Hammerschlag method와 Schmaltz and Peiper's method가 활용되었지만 대중적으로 사용되지는 않았다. 가스정량법은 van Slyke 장치를 이용하여 간접적으로 헤모글로빈 농도를 추정하는 방법으로 가장 정확한 결과를 제공하였다. 본 조사에서는 19세기 후반과 20세기 초 중반까지 사용된 혈색소 측정기기와 측정법에 대한 특징 및 제한점 등을 알아 보았고, 이를 통하여 현재의 측정방법 및 새롭게 등장하는 기술들에 대한 이해와 응용력을 향상시키고자 하였다. 또한 이번 조사가 진단검사 기술분야의 다양한 역사유물에 대한 고찰뿐만 아니라 역사자료 체계화에 대한 필요성을 자각하는 시발점이 되기를 희망한다.

Keywords

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