The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Bacillus spp. Having Gelatin Decomposing Activity on Root-knot Nematode, Meloidogyne incognita on Pepper

젤라틴 분해력이 있는 Bacillus균의 고추 고구마뿌리혹선충에 미치는 영향

  • Kim, Jin Han (Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Park, Chan Sun (Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Young-Ho (Kroea Stevia Research Institute) ;
  • Kim, Yu Ri (Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Seung Woong (Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Rho, Mun Chual (Natural Product Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김진한 (한국생명공학연구원 천연물소재연구센터) ;
  • 박찬선 (한국생명공학연구원 천연물소재연구센터) ;
  • 김영호 (한국스테비아(주)) ;
  • 김유리 (한국생명공학연구원 천연물소재연구센터) ;
  • 이승웅 (한국생명공학연구원 천연물소재연구센터) ;
  • 노문철 (한국생명공학연구원 천연물소재연구센터)
  • Received : 2016.10.14
  • Accepted : 2016.11.29
  • Published : 2016.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study investigated the decomposing activities on gelatin and egg sac of root-knot nematode (Meloidogyne incognita). Ten Bacillus isolates (KRB-1~10) isolated from the soils of Mt. Naejang. Among them, KRB-5, 9, and 10 showed decomposing activities, and identified as Bacillus subtilis KRB-5, Bacillus amyloliquefaciens KRB-9, and KRB-10 by 16S rRNA sequence analysis, respectively. Under pot experiments using pepper, 100-fold diluted culture broth of three isolates reduced the number of egg sac on roots collected 60 days and 90 days and increased the growth of aerial part compared with the group only treated with Meloidogyne incognita. Especially, the group treated B. subtilis KRB-5 was superior in the growth of pepper. These results suggest that the B. subtilis KRB-5 can be used to control the root-knot nematode on pepper.

내장산 토양에서 분리한 미생물들의 젤라틴 및 고구마뿌리혹선충의 난낭 분해활성을 평가하였다. 활성이 우수한 3종의 균주는 16S rRNA 염기서열을 이용하여 분석하였으며, Bacillus subtilis KRB-5와 Bacillus amyloliquefaciens KRB-9, 10이 분리동정되었다. 고구마뿌리혹선충에 20일간 노출시킨 고추모종에 분리동정된 3종의 미생물을 90일동안 처리하고 난낭과 고추모종의 지상부 생육에 미치는 영향을 조사하였다. 실험결과 B. subtilis KRB-5을 처리한 군에서는 미생물 무처리 군 및 다른 미생물 처리 군과 비교하여 고구마뿌리혹선충의 난낭 수가 현저하게 감소하였으며, 고추의 지상부 생육도 개선되는 것을 확인하였다. 따라서 B. subtilis KRB-5 균주를 이용한 박과 작물에 대한 확대 효능 평가와 시설 재배지 현장 시험 등의 추가적인 연구는 활용할 필요할 것으로 사료된다.

Keywords

References

  1. Bonants, P. J. M., P. F. L. Fitters, H. Thijs, E. den belder, C. Waalwijk and J. W. D. Henfling (1995) A basic serine protease from Paecilomyces lilacinus with biological activity against Meloidogyne halpa eggs. Micorobiology. 141(4):775-784.
  2. Chitwood, D. (2002) Phytochemical based strategies for nematode control. Annu. Rev. Phytopathol. 40:221-249. https://doi.org/10.1146/annurev.phyto.40.032602.130045
  3. Choo, H. Y., S. M. Lee, J. B. Kim and Y. D. Park (1990) Relationship of root-knot nematode, Meloidogyne incognita to pathogenesis of Rhizoctonia solani on cucumber, pepper, and tomato. Korean J. Plant Pathol. 6(3):409-411.
  4. Geeta, S. (2004) Biocontrol of nematode-borne diseases in vegetable crops. In: Mukerji K. G. (ed.), Fruit and vegetable diseases, pp. 397-450. Kluwer Academic Publishers, USA.
  5. Ha, W. J., Y. C. Kim, H. C. Jung and S. K. Park (2014) Control of the root-knot nematode (Meloidogyne spp.) on cucumber by a liquid bio-formulation containing chitinolytic bacteria, chitin and their products. Res. Plant Dis. 20(2):112-118. https://doi.org/10.5423/RPD.2014.20.2.112
  6. Kim, D. G. and J. H. Lee (2008) Allium tuberosum, a new host of root-knot nematode, Meloidogyne incognita in Korea. Res. Plant Dis. 14(1):76-78. https://doi.org/10.5423/RPD.2008.14.1.076
  7. Kim, D. G. and J. H. Lee (2008) Economic threshold of Meloidogyne incognita for greenhouse grown cucumber in Korea. Res. Plant Dis. 14:117-121. https://doi.org/10.5423/RPD.2008.14.2.117
  8. Kim, D. G., H. S. Kim, S. H. Kim, J. H. Lee, and J. T. Yoon (2004) Occurrence of root-knot nematode on Dendranthema x grandiflorum and influences on plant growth. Res. Plant Dis. 10(1):25-29. https://doi.org/10.5423/RPD.2004.10.1.025
  9. Kim, D. G., T. Y. Kwon, Y. H. Ryu, L. K. Yeon and C. S. Huh (2012) Resistance of commercial pepper cultivars to root-knot nematodes. Res. Plant Dis. 18(4):370-375 https://doi.org/10.5423/RPD.2012.18.4.370
  10. Kim, D. G., Y. K. Lee and B. Y. Park (2001) Root-knot nematode species distributing in greenhouses and their simple identification scheme. Res. Plant Dis. 7:49-55.
  11. Kim, H. H., M. R. Cho, T. J. Kang, J. A. Jung and Y. K. Han (2010) Screening of tomato cultivars resistant to root-knot nematode, Meloidogyne incognita. Res. Plant Dis. 16:294-298. https://doi.org/10.5423/RPD.2010.16.3.294
  12. Kim, J. I. and S. C. Han (1988) Effect of solarization for control of root-knot nematode (Meloidogyne spp.) in vinyl house. Korean J. Appl. Entomol. 27(1):1-5.
  13. Moon, H. S., Z. Khan, S. G. Kim, S. H. Son and Y. H. Kim (2010) Biological and structural mechanisms of disease development and resistance in chili pepper infected with the root-knot nematode. Plant Pathol. J. 26(2):149-153. https://doi.org/10.5423/PPJ.2010.26.2.149
  14. Oka, Y., B. Ben-Daniel and Y. Cohen (2012) Nematicidal activity of the leaf powder and extracts of Myrtus communis against the root-knot nematode Meloidogyne javanica. Plant Pathol. 61:1012-1020. https://doi.org/10.1111/j.1365-3059.2011.02587.x
  15. Padgham, J. L. and R. A. Sikora (2007) Biological control potential and modes of action of Bacillus megaterium against Meloidogyne graminicola on rice. Crop protection. 26(7):971-977. https://doi.org/10.1016/j.cropro.2006.09.004
  16. Park, S. D., S. D. Park, T. Y. Kwon, B. S. Choi, W. S. Lee and Y. E. Choi (1995) Study on integrated control against root knot nematode of fruit vegetables (Oriental melon and cucumber) in vinyl house. Korea J. Appl. Entomol. 34:75-81.
  17. Pavaraj, M., G. Bakavathiappan and S. Baskaran (2012) Evaluation of some plant extracts for their nematicidal properties against root-knot nematode, Meloidogyne incognita. J. Biopest. 5:106-110.