• Asian Journal of Turfgrass Science
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• v.12 no.2
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• pp.107-112
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• 1998

우리나라 골프장의 잔디 가해 해충과 천적을 조사하던 중 농림작물의 중요해충인 고구마뿌리혹선충을 Zoysia잔디에서 발견하였다. 고구마뿌리혹선충에 감염된 잔디는 새 뿌리가 발육하지 못하고 뭉툭하게 되어 황화현상과 함께 전반적으로 생육이 부진하였다. 감염된 잔디의 종은 들잔디(Zoyais japonica)와 금잔디(Zoysia matrella), 버뮤다글라스(Cynodon sp.)였다. 그리고 Z. japonica는 고구마뿌리혹선충의 새로운 기주로 기록된다. 조사 골프장의 대부분이 정도의 차이는 있었지만 피해를 받고 있었다. 잔디에서 뿌리혹선충이 기록되기는 우리나라에서는 처음이다.

• Research in Plant Disease
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• v.23 no.4
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• pp.348-354
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• 2017

To investigate occurrence of root-knot nematode (RKN) in plastic house of central area of Korea, 132 soil samples were collected in cucumber, water melon, tomato, red pepper and strawberry fields from 2013 to 2015. Among 132 soil samples, 65 soil samples (49%) were infested with RKN and mean density of RKN was 178 second-stage juveniles per $100cm^3$ soil (min. 1 ~ max. 3,947). The frequency of RKN by regional was the highest in Chuncheon with 80%, followed by Cheonan (68%), Nonsan (36%), Buyeo (33%) and Yesan (30%). The frequency of RKN by crops was the highest in tomato with 83%, followed by cucumber (61%), strawberry (41%), red pepper (30%), watermelon (26%). To identify the species of RKN, fifteen populations were selected for representative populations. As a phylogenetic analysis of 15 populations, southern root-knot nematode (Meloidogyne incognita), peanut root-knot nematode (M. arenaria) and northern root-knot nematode (M. hapla) were identified with 47%, 20% and 33% ratio, respectively. In crops, M. incognita, M. arenaria and M. hapla were detected in tomato, M. incognita and M. arenaria were detected in cucumber and watermelon, and M. hapla was detected in strawberry and lettuce. Thus, there should be a continuous management to major species of each crops to prevent dispersal of RKN damages.

• Korean Journal of Organic Agriculture
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• v.30 no.3
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• pp.393-407
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• 2022

The effect of Neobacillus sp. JC05 extract on the defense response in cucumber plants against root-knot nematode (RKN) was evaluated. As a result of drench treatment of JC05-extract in cucumber plants, formation of egg mass per plants and disease severity were significantly decreased compared to untreated control plants; the malondialdehyde contents also decreased in JC05-extract treated plants. When eggs of Meloidogyne incognita were inoculated, cucumber plants treated with JC05-extract elevated pathogenesis-related gene expression such as chitinase and lipoxygenase, these are well known as inducing resistance in plants, in addition, peroxidase among antioxidant enzymes was significantly activated. Moreover, the JC05-extract enhanced FDAse activity in soils grown cucumber plants inoculated by eggs of M. incognita. Taken together, these results suggest that the JC05-extract could involve in activation of defense-related mechanisms of cucumber plants and result in decrease of disease occurrence caused by M. incognita.

• The Korean Journal of Pesticide Science
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• v.19 no.2
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• pp.101-105
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• 2015

Efficacy of novel nematicide, imicyafos GR was evaluated against two species of the root-knot nematodes in pot and greenhouse conditions. When tested in pots, the population of Meloidogyne incognita and M. hapla was reduced sixty days after treatment, with mortality rate of 91.5% and 90.6%, respectively. Suppression effect of imicyafos GR on root galling in tomato was tested. The number of root galls caused by M. incognita and M. hapla was reduced 60 days after nematode inoculation, with the efficacy of 94.2% and 95.1%, respectively. Under greenhouse conditions planted with watermelon, melon, cucumber, and tomato, the efficacy of imicyafos GR on M. incognita persisted up to 60 days after treatment, showing 90% of control efficacy. Moreover, the number of root galling was more reduced than fosthiazate treatment, with the potential as a control agent.

• Korean journal of applied entomology
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• v.45 no.2 s.143
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• pp.211-216
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• 2006

Total of 36 sweet potato field soils were sampled to survey the occurrence of the root-knot nematodes (RKN). The 61% of sweet potato fields in Haenam, 40% in Iksan and 31% in Yeju were infested with RKN, respectively. Average population density of RKN was 324 juveniles per 300 g soil. The resistance screening of sweet potato cultivars against RKN was carried out by using clay pots in a greenhouse. Average temperature under ground 10 cm in pot was 21.5$^{\circ}C$ during the test. There was no difference in number of egg sacs among different inoculation methods, however the egg-inoculation method was easy for treatlnent and had stable far results. The multiplication ratio of Melioidogyne incognita differed from 6.3 times (Jeungmi) to 63.2 times (Yulmi) by sweet potato cultivas. There were no cultivars showing resistance to M. incognita, but Jinmi, Jeungmi and Borami had resistance to if arenaria, M. hapla and M. javanica.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.123-129
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• 2000

This study was conducted to analyse the distribution of root-knot nematodes (Meloidogyne spp.) in fruit vegetable production areas in Korea. Soil samples were collected from greenhouses in Sungju (Kyungpook), Yeoju (Kyungki), Haman (Kyungnam), and Chungwon (Choongpook) provinces in 1997-1999. Plant parasitic nematodes were separated for density counting and some of the root-knot nematodes were identified using enzyme phenotypes of malate dehydrogenase (MDH) and esterase (EST). Among the 185 farms in Sungju province, Meloiciogyne spp. were detected from 99 farms (53.5%). Other plant parasitic nematodes detected were; Helic~otylmchuss pp. from 7 farms, Aphelenchus spp. from 43 farms, and Criconematids from 26 farms. Using the female enzyme phenotypes of MDH and EST. the four major root-knot nematodes in Korea, M. incognitc~(M I), M. uretznri~(M~ A), M. huplu (MH), and M. juvunica, could be identified. In the enzyme phenotype identification of 13 populations collected from Sunnam in Sungju province, 6 populations were identified as MA, 5 populations were identified as MI, and 2 populations were mixed with MI and MA. Among the 6 populations from Chojun in Sungju province. 4 populations were MA, one population was MI, and one population showed enzyme phenotypes of unknown species. Among the 14 populations of Yeoju province, l I populations were MH and 3 populations were MA.

• Korean journal of applied entomology
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• v.53 no.1
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• pp.85-91
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• 2014

In this study, we analyzed the phylogenetic characterization of root-knot nematodes (Meloidogyne spp.) in soils from fruits and vegetables greenhouses in Korea. Soil samples were collected from 12 greenhouse fields in which tomato, cucumber, watermelon, and Oriental melon were being cultivated. Meloidogyne spp. were detected in all the soil samples at an average number of $72{\pm}6$ nematodes/300 g of soil to $2,898{\pm}468$ nematodes/300 g of soil. Phylogenetic analysis using polymerase chain reaction-restriction fragment length polymorphism was attempted for the second-stage juveniles (J2) of Meloidogyne spp. collected from the greenhouse soils. Twelve Meloidogyne spp. from the greenhouse soils were classified into two groups by using HinfI digestion of mitochondrial DNA, resulting in 900, 410, 290, and 170 bp fragments (group A) and 900, 700, and 170 bp fragments (group B). Phylogenetic analysis based on mitochondrial DNA sequences (1,483-1,521 bp) showed that nine group A isolates were identified as Meloidogyne incognita (99.73-99.93%) and three group B isolates showed 99.54-99.73% similarity to Meloidogyne arenaria.

• Proceedings of the Korean Society of Soil Zoology Conference
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• 2001.10a
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• pp.18-18
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• 2001

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.247-252
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• 2012

This study examined the control effects of amino acid biochemical agent extracted from chicken feathers on Meloidogyne incognita for the purpose of developing an environmentally friendly nematicidal agent that can replace chemical control of root-knot nematodes (RKN). We investigated the lethal effects of J2 juveniles for 19 types of commercial amino acids. As a result, five kinds of amino acids (L-asparagine, L-aspartic acid, L-methionine, L-tyrosine, L-cysteine) showed mortality rate of more than 50% at a concentration of 50 mM. L-asparagine showed the highest mortality rate at 94%. We also investigated the lethal effect of J2 juveniles and suppressive effects of egg hatching by feather amino acids (FAA) biochemical agent. It showed that the mortality rate of J2 juveniles was more than 80% and suppression rate of egg hatching was 74% at 1/50 concentrations of FAA. As a result of conducting a tomato pot culture experiment for 60 days after treating 1/50 concentrations of FAA biochemical agent in rhizosphere soil, it showed that the control effects were 63% of juveniles density in the soil, 59% of egg mass and 61% of root gall index, respectively. Based on the above results, it is considered that the FAA biochemical agent extracted from chicken feathers can be used as an environmentally friendly nematicidal agent of RKN.

• Korean journal of applied entomology
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• v.39 no.2
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• pp.111-115
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• 2000

Fifty-two nematode predatory fungi were isolated from 37 soil samples collected from eight provinces in Korea. Isolated fungi were tested their predacity against Rhabditis sp. and Meloidogyne incognita in petri dish, and against M. incognita in greenhouse pot experiments. Fifty isolates had trapping organ of adhesive networks and two isolates had adhesive column or adhesive knob. In petri dish experiments, 5 1 isolates against Rhubditis sp. and 26 isolates against M. incognita showed over 91 % of predacity; in greenhouse experiments, however, only three isolates showed over 81% of predacity. These results imply that the results from the laboratory experiments are not consistent with those from the greenhouse experiments. Therefore, to select a promising biocontrol predatory fungi for plant-parasitic nematodes, the screening experiment should be conducted in conditions close to nature.