Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지
DOI QR코드

DOI QR Code

Bactericidal Efficacy of a Powder Disinfectant Containing Phosphate Compounds Against Salmonella Typhimurium

  • Park, Eun-Kee (Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University) ;
  • Cha, Chun-Nam (Engineering Research Institute, Department of Industrial Systems Engineering, Gyeongsang National University) ;
  • Yoo, Chang-Yeul (Department of Computer Information, Gyeongnam Provincial Namhae College) ;
  • Kim, Suk (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • Received : 2016.09.09
  • Accepted : 2016.09.26
  • Published : 2016.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Salmonella Typhimurium (S. Typhimurium) is one of the common food pathogens which may cause gastroenteritidis in human and animals. The microorganism also causes the economic loss in animal farming and food industry. In this study, the disinfection efficacy of a powder disinfectant containing phosphate compounds as a main ingredient, was evaluated against S. Typhimurium. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at $4^{\circ}C$. The disinfectant and test bacteria were diluted with hard water (HW) or organic matter suspension (OM) according to treatment condition. On HW and OM conditions, the bactericidal activity of the disinfectant against S. Typhimurium was 4- and 2-fold dilutions, respectively. As the disinfectant composed to phosphate compounds possesses bactericidal efficacy against pathogenic bacteria such as S. Typhimurium, the powder disinfectant can be used to control the spread of bacterial diseases.

살모넬라증은 가축에 심각한 피해를 유발하는 질병으로, 축산업과 식품산업에 많은 경제적 손실을 초래하고 있다. 본 연구에서는, 복합 인산염을 주성분으로 하는 분말 소독제의 Salmonella Typhimuriums에 대한 살균효과 시험을 수행하였다. 배지희석법을 이용한 살균효력시험은 $4^{\circ}C$에서 30분 동안 시험 세균을 희석 소독제에 노출시켜 소독제의 가장 효과적인 낮은 희석배수를 결정하는 시험이다. 본 분말 소독제와 시험 세균을 처리조건에 따라 경수와 유기물로 희석하여 반응을 시켰다. 유기물 조건에서, Salmonella Typhimurium에 대한 소독제의 살균력은 경수조건에서의 살균력과 비교하여 낮게 나타났는데, 이는 유기물들에 의한 소독제의 살균 유효성분에 대한 저해작용에 따른 것으로 사료된다. 분말 소독제는 Salmonella Typhimurium과 같은 병원체에 대해 살균효과를 가지므로, 살모넬라증과 같은 세균성 질병의 확산을 제어하는데 효과적으로 이용될 수 있을 것으로 사료된다.

Keywords

References

  1. Cleaveland, S., Laurenson, M.K. and Taylor. L.H.: Diseases of humans and their domestic mammals: pathogen characteristics, host range and the risk of emergence. B. Biol. Sci., 356, 991-999 (2001). https://doi.org/10.1098/rstb.2001.0889
  2. Kim, G.S., Kim, D.H., Lim, J.J., Lee, J.J., Han, D.Y., Lee, W.M., Jung, W.C., Min, W.G., Won, C.G., Rhee, M.H., Lee, H.J. and Kim, S.: Biological and antibacterial activities of the natural herb Houttuynia cordata water extract against the intracellular bacterial pathogen Salmonella within the raw 7 macrophage. Biol. Pharm. Bull., 31, 2012-2017 (2009).
  3. Kim, D.H., Lim, J.J., Lee, J.J., Jung, W.C., Shin, H.J., Lee, H.J., Kim, G.S. and Kim, S.: Antibacterial and therapeutic effects of Houttuynia cordata ethanol extract for murine salmonellosis. Kor. J. Environ. Agricul., 27, 156-162 (2008). https://doi.org/10.5338/KJEA.2008.27.2.156
  4. Doughari, J.H.: Antimicrobial activity of Tamarindus indica L. Trop. J. Pharm. Res., 5, 597-603 (2006).
  5. Doughari, J.H and Okafor, N.B.: Antibacterial activity of Senna siamae leaf extracts on Salmonella typhi. Afr. J. Microbiol. Res., 2, 42-46 (2008)
  6. Ibrahim, M.A., Emeash, H.H., Ghoneim, N.H. and Abdel- Halim, M.A.: Seroepidemiological studies on poultry salmonellosis and its public health importance. J. World's Poult. Res., 3, 18-23 (2013).
  7. Katsuda, K., Kohmoto, M., Kawashima, K. and Tsunemitsu, H.: Frequency of enteropathogen detechtion in sucking and weaned pigs with diarrhea in Japan. J. Vet. Diagn. Invest., 18, 350-354 (2006). https://doi.org/10.1177/104063870601800405
  8. Korsak, N., Jacob, B., Groven, B., Etienne, G., China, B., Ghafir, Y. and Daube, G.: Salmonella contamination of pigs and pork in an integrated pig production system. J. Food Prot., 66, 1126-1133 (2003). https://doi.org/10.4315/0362-028X-66.7.1126
  9. Giammanco, G., Pignato, S. and Giammanco, G.M.: Recent trends in salmonellosis epidemiology. J. Prev. Med. Hyg., 40, 19-24 (1999).
  10. Scharff, R.L.: Economic burden from health losses due to foodborne illness in the United States. J. Food Prot., 75, 123-131 (2012). https://doi.org/10.4315/0362-028X.JFP-11-058
  11. Whitehead, R.N., Overton, T.W., Kemp, C.L. and Webber, M.A.: Exposure of Salmonella enteric serovar Typhimurium to high level biocide challenge can select multidrug resistant mutants in a single step. PLoS ONE, 6, e22833 (2011). https://doi.org/10.1371/journal.pone.0022833
  12. Park, E.K., Cho, Y. and Lee, H.J.: Bactericidal efficacy of a disinfectant solution composed to povidine-iodine against Salmonella typhimurium and Brucella ovis. J. Fd Hyg. Safety, 29, 165-169 (2014). https://doi.org/10.13103/JFHS.2014.29.3.165
  13. Russell, A.D.: Biocide use and antibiotic resistance: the relevance of laboratory findings to clinical and environmental situations. Lancet Infect. Dis., 3, 794-803 (2003). https://doi.org/10.1016/S1473-3099(03)00833-8
  14. Ahmad, K.: Control of animal diseases caused by bacteria: Principles and approaches. Pak. Vet. J., 25, 200-202 (2005).
  15. Jeffrey, D.J.: Chemicals used as disinfectants: active ingredients and enhancing additives. Rev. Sci. Tech. Off. Int. Epiz., 14, 57-74 (1995). https://doi.org/10.20506/rst.14.1.828
  16. Suarez, V.B., Frison, L., De Basilico, M.Z., Riveira, M. and Reinheimer, J.A.: Inhibitory activity of phosphates on molds isolated from foods and food processing plants. J. Food Prot., 68, 2475-2479 (2005). https://doi.org/10.4315/0362-028X-68.11.2475
  17. Long, N.H.B.S., Gal, R. and Bunka, F.: Use of phosphates in meat products. Afr. J. Biotechnol., 10, 19874-19882 (2011).
  18. Bunkova, L., Pleva, P., Bunka, F., Valasek, P. and Kracmar, S.: Antibacterial effects of commercially available phosphates on selected microorganisms. Acta Univ. Agric. et Silvic. Mendel. Brun., 56, 19-24 (2008).
  19. Kar, A.: Pharmaceutical microbiology. New Age International (P) Ltd., New Delhi, pp. 219 (2008).
  20. Gottardi, W. and Nagl, M.: Chlorine covers on living bacteria: the initial step in antimicrobial action of active chlorine compounds. J. Antimicrob. Chemother., 55, 475-482 (2005). https://doi.org/10.1093/jac/dki054
  21. Obritsch, J.A., Ryu, D., Lampila, L.E. and Bullerman, L.B.: Antibacterial effects of long-chain polyphosphates on selected spoilage and pathogenic bacteria. J. Food Prot., 71, 1401-1405 (2008). https://doi.org/10.4315/0362-028X-71.7.1401
  22. Varkey, A.J.: Antibacterial properties of some metals and alloys in combating coliforms in contaminated water. Sci. Res. Essays, 5, 3834-3839 (2010).
  23. Lemire, J.A., Harrison, J.J. and Turner, R.J.: Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat. Rev. Microbiol., 11, 371-384 (2013). https://doi.org/10.1038/nrmicro3028
  24. Jolli, J. and Jacob, C: Sustainability management for reducing the health hazards in the indoor swimming pools of UAE. ASS, 11, 124-135 (2015).
  25. Mahfouz, A.B., Atilhan, S., Batchelor, B., Linke, P., Abel- Wahab, A. and El-Halwagi, M.: Optimal scheduling of biocide dosing for seawater-cooled power and desalination plants. Clean Technol. Envir., 13, 783-796 (2011). https://doi.org/10.1007/s10098-011-0352-6
  26. Shirron, N., Kisluk, G., Zelikovich, Y., Eivin, I., Shimoni, E. and Yaron, S.: A comparative study assaying commonly used sanitizers for antimicrobial activity against indicator bacteria and a Salmonella Typhimurium strain on fresh produce. J. Food Prot., 72, 2413-2417 (2009). https://doi.org/10.4315/0362-028X-72.11.2413
  27. Tornuk, F., Cankurt, H., Ozturk, I., Sagdic, O., Bayram, O. and Yetim, H.: Efficacy of various plant hydrosols as natural food sanitizers in reducing Escherichia coli O157:H7 and Salmonella Typhimurium on fresh cut carrots and apples. Int. J. Food Microbiol., 148, 30-35 (2011). https://doi.org/10.1016/j.ijfoodmicro.2011.04.022
  28. Choi, T.Y.: Biocidal effect of a sanitizer/disinfectant, $Foodsafe^{(R)}$, against bacteria, yeast, and mycobacteria. Korean J. Clin. Microbiol., 11, 117-122 (2008). https://doi.org/10.5145/KJCM.2008.11.2.117