Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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In-vitro Anticancer and Antioxidant Activity of Gold Nanoparticles Conjugate with Tabernaemontana divaricata flower SMs Against MCF -7 Breast Cancer Cells

  • Preetam Raj, J.P (Department of Plant Biology and Biotechnology, PG Biotechnology, Loyola College) ;
  • Purushothaman, M (Department of Plant Biology and Biotechnology, PG Biotechnology, Loyola College) ;
  • Ameer, Khusro (Department of Plant Biology and Biotechnology, PG Biotechnology, Loyola College) ;
  • Panicker, Shirly George (Department of Plant Biology and Biotechnology, PG Biotechnology, Loyola College)
  • Received : 2015.02.21
  • Accepted : 2015.06.09
  • Published : 2016.02.01
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Abstract

Biologically stabilized gold nanoparticles were synthesized from the flower aqueous extract of T. divaricata. The synthesized nanoparticles were characterized by UV-Vis spectrophotometer, Zeta sizer, FTIR and TEM analysis. T. divaricata reduced gold nanoparticles having particle size and potential of 106.532 nm and -10.2 mV, respectively, with a characteristic peak of 550 nm in UV-visible spectrophotometer. FTIR graph after comparison between the crude flower extract and gold nanoparticles showed three major shifts in the functional groups. The morphology and size of the gold nanoparticles were examined by HRTEM analysis, which showed that most of the nanoparticles were nearly spherical with size of 100 nm. The gold nanoparticles synthesized demonstrated potent anticancer activity against MCF-7 cell line. The findings conclude that the antioxidant molecule present in T. divaricata may be responsible for both reduction and capping of gold nanoparticles which possess potential applications in medicine and pharmaceutical fields.

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