Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Production of Total Reducing Sugar from Enteromorpha intestinalis Using Citrate Buffer Pretreatment and Subsequent Enzymatic Hydrolysis

창자파래로부터 citrate buffer를 이용한 전처리와 효소가수분해를 통한 환원당 생산

  • Kim, Dong-Hyun (Department of Biotechnology, Pukyong National University) ;
  • Kim, A-Ram (Department of Biotechnology, Pukyong National University) ;
  • Park, Don-Hee (Department of Biotechnology and Bioengineering, Chonnam National University) ;
  • Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
  • Received : 2015.02.05
  • Accepted : 2015.05.19
  • Published : 2016.02.01
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Abstract

In this study, the effects of citrate buffer pretreatment conditions (solid-to-liquid ratio, reaction temperature, pH and concentration of buffer) on enzymatic hydrolysis of E. intestinalis for total reducing sugar (TRS) production were investigated. As a results of the citrate buffer pretreatment, a 5.40% hydrolysis yield was obtained under conditions including 1:10 solid-to-liquid ratio, 0.25 M citrate buffer (pH 3.5) at $140^{\circ}C$ for 60 min. The maximum hydrolysis yield of 18.68% was obtained to enzymatic hydrolysis after pretreatment. This result is 1.81 times higher than that of control.

본 연구에서는 창자파래(Enteromorpha intestinalis)로부터 citrate buffer를 사용하여 전처리 조건(고액비, 반응온도, buffer의 pH와 농도)에 따른 전처리 반응과 효소가수분해를 통한 가수분해 수율을 조사하였다. 0.25 M, pH 3.5의 citrate buffer를 이용하여 $140^{\circ}C$에서 60분간 전처리를 수행한 결과, 5.40%의 가수분해 수율을 얻었다. 최종적으로 전처리 반응 후 24시간의 효소 가수분해를 통하여 18.68%의 가수분해 수율을 얻었다. 이 결과는 대조구에 비하여 약 1.81배 증가한 결과이다.

Keywords

References

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