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http://dx.doi.org/10.9713/kcer.2015.54.1.70

Production of Total Reducing Sugar from Enteromorpha intestinalis Using Citrate Buffer Pretreatment and Subsequent Enzymatic Hydrolysis  

Kim, Dong-Hyun (Department of Biotechnology, Pukyong National University)
Kim, A-Ram (Department of Biotechnology, Pukyong National University)
Park, Don-Hee (Department of Biotechnology and Bioengineering, Chonnam National University)
Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
Publication Information
Korean Chemical Engineering Research / v.54, no.1, 2016 , pp. 70-74 More about this Journal
Abstract
In this study, the effects of citrate buffer pretreatment conditions (solid-to-liquid ratio, reaction temperature, pH and concentration of buffer) on enzymatic hydrolysis of E. intestinalis for total reducing sugar (TRS) production were investigated. As a results of the citrate buffer pretreatment, a 5.40% hydrolysis yield was obtained under conditions including 1:10 solid-to-liquid ratio, 0.25 M citrate buffer (pH 3.5) at $140^{\circ}C$ for 60 min. The maximum hydrolysis yield of 18.68% was obtained to enzymatic hydrolysis after pretreatment. This result is 1.81 times higher than that of control.
Keywords
Enteromorpha intestinalis; Citrate buffer pretreatment; Enzymatic hydrolysis; Total reducing sugar;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
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