The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-adipogenesis Effects of 3 Herbal Formula on Blood Stasis

어혈처방 3종이 지방전구세포 분화에 미치는 영향

  • Lee, Hoyoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine) ;
  • Shim, Eun Hyoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Myeong Soo (Clincal Reserach Division, Korea Institute of Oriental Medicine) ;
  • Lee, Ju Ah (KM Fundamental Research Division, Korea Institute of Oriental Medicine)
  • 이호영 (한국한의학연구원 한의기반연구부) ;
  • 심은형 (한국한의학연구원 한의기반연구부) ;
  • 이명수 (한국한의학연구원 임상연구부) ;
  • 이주아 (한국한의학연구원 한의기반연구부)
  • Received : 2016.10.07
  • Accepted : 2016.11.16
  • Published : 2016.11.30
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Abstract

Objectives : Blood stasis (BS) is related to be caused by blood circulation and stagnation which are cancer, atherosclerosis and hyperlipidemia in traditional medicine. We extracted 3 kinds of BS formula; Seogakjihwang-tang (SGT), Tonggyuhawlhyul-tang (THT), Hyulbuchukeo-tang (HCT). This study was conducted to investigate whether the 3 kinds of herbal formula extracts have inhibitory efficacy association with anti-adipogenesis. Methods : To investigate the anti-adipogenesis, we used the mouse fibroblast cell line, 3T3-L1 which differentiated into adipocytes in response to insulin, IBMX and dexamethasone (MDI). Cytotoxicity of herbal formula extracts were examined by CCK-8 kit. Intracellular lipid droplets were detected by Oil-Red-O staining. Triglyceride (TG) and leptin were measure using elisa kit. Results : The yield of water extracts was 14.62% (SGT), 21.27% (THT), 20.02% (HCT). Lipid accumulation was reduced significantly by 3 kinds of herbal formula compared to control. Especially, THT and HCT decreased lipid droplet, respectively at all concentration. The TG and leptin were also inhibited by 3 kinds of herbal formula. The IC50 of TG were $280.51{\mu}g/m{\ell}$ (SGT), $52.62{\mu}g/m{\ell}$ (THT), $313.99{\mu}g/m{\ell}$ (HCT). The IC50 of leptin were $348.76{\mu}g/m{\ell}$ (SGT), $164.02{\mu}g/m{\ell}$ (THT), $257.00{\mu}g/m{\ell}$ (HCT). THT was better than other herbal formula on anti-adipogenesis. Conclusion : kinds of herbal formula inhibited adipogenesis in MDI-induced 3T3-L1 adipocytes, as indicated by the significant reduction in TG and leptin concentration without cytotoxicity. Therefore, 3 kinds of herbal formula for BS might act as a therapeutic agent for preventing lipid diseases, such as obesity and atherosclerosis.

Keywords

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