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http://dx.doi.org/10.6116/kjh.2016.31.6.29.

Anti-adipogenesis Effects of 3 Herbal Formula on Blood Stasis  

Lee, Hoyoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine)
Shim, Eun Hyoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine)
Lee, Myeong Soo (Clincal Reserach Division, Korea Institute of Oriental Medicine)
Lee, Ju Ah (KM Fundamental Research Division, Korea Institute of Oriental Medicine)
Publication Information
The Korea Journal of Herbology / v.31, no.6, 2016 , pp. 29-35 More about this Journal
Abstract
Objectives : Blood stasis (BS) is related to be caused by blood circulation and stagnation which are cancer, atherosclerosis and hyperlipidemia in traditional medicine. We extracted 3 kinds of BS formula; Seogakjihwang-tang (SGT), Tonggyuhawlhyul-tang (THT), Hyulbuchukeo-tang (HCT). This study was conducted to investigate whether the 3 kinds of herbal formula extracts have inhibitory efficacy association with anti-adipogenesis. Methods : To investigate the anti-adipogenesis, we used the mouse fibroblast cell line, 3T3-L1 which differentiated into adipocytes in response to insulin, IBMX and dexamethasone (MDI). Cytotoxicity of herbal formula extracts were examined by CCK-8 kit. Intracellular lipid droplets were detected by Oil-Red-O staining. Triglyceride (TG) and leptin were measure using elisa kit. Results : The yield of water extracts was 14.62% (SGT), 21.27% (THT), 20.02% (HCT). Lipid accumulation was reduced significantly by 3 kinds of herbal formula compared to control. Especially, THT and HCT decreased lipid droplet, respectively at all concentration. The TG and leptin were also inhibited by 3 kinds of herbal formula. The IC50 of TG were $280.51{\mu}g/m{\ell}$ (SGT), $52.62{\mu}g/m{\ell}$ (THT), $313.99{\mu}g/m{\ell}$ (HCT). The IC50 of leptin were $348.76{\mu}g/m{\ell}$ (SGT), $164.02{\mu}g/m{\ell}$ (THT), $257.00{\mu}g/m{\ell}$ (HCT). THT was better than other herbal formula on anti-adipogenesis. Conclusion : kinds of herbal formula inhibited adipogenesis in MDI-induced 3T3-L1 adipocytes, as indicated by the significant reduction in TG and leptin concentration without cytotoxicity. Therefore, 3 kinds of herbal formula for BS might act as a therapeutic agent for preventing lipid diseases, such as obesity and atherosclerosis.
Keywords
Blood stasis; Seogakjihwang-tang (Xijiao Dihuang Decoction); Tonggyuhawlhyul-tang (Tongqiaohuoxue Decoction); Hyulbuchukeo-tang (Xuefuzhuyu decoction); anti-adipogenesis;
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Times Cited By KSCI : 3  (Citation Analysis)
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