Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Effect of Nitrogen Deficiency on Cell Growth and Fatty Acids Production of Nannochloropsis oculata K-1281

질소원 고갈조건에 대한 Nannochloropsis oculata K-1281의 세포 성장과 지방산 생산 연구

  • Hong, Seong-Joo (National Marine Bioenergy Center & Dept. of Biological Engineering, Inha University) ;
  • Yim, Narae (National Marine Bioenergy Center & Dept. of Biological Engineering, Inha University) ;
  • Han, Mi-Ae (National Marine Bioenergy Center & Dept. of Biological Engineering, Inha University) ;
  • Yoo, Danbee (National Marine Bioenergy Center & Dept. of Biological Engineering, Inha University) ;
  • Lee, Choul-Gyun (National Marine Bioenergy Center & Dept. of Biological Engineering, Inha University)
  • 홍성주 (인하대학교 해양바이오에너지센터 & 생명공학과) ;
  • 임나래 (인하대학교 해양바이오에너지센터 & 생명공학과) ;
  • 한미애 (인하대학교 해양바이오에너지센터 & 생명공학과) ;
  • 유단비 (인하대학교 해양바이오에너지센터 & 생명공학과) ;
  • 이철균 (인하대학교 해양바이오에너지센터 & 생명공학과)
  • Received : 2016.10.18
  • Accepted : 2017.01.09
  • Published : 2016.12.31
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Abstract

Most of microalgae shift their metabolic pathways toward the fatty acid biosynthesis following nitrogen deprivation. Recent studies on Nannochloropsis species, oleaginous microalgae, have been performed to investigate the regulation of contents and compositions of fatty acids under stressful condition. The objective of this experiment is to identify the effect of nitrogen on cell growth and fatty acids production in Nannochloropsis oculata K-1281 and compare fatty acid composition response to nitrogen deficiency between N. oculata LB2164 and K-1281. The fatty acids content in N. oculata K-1281 was increased up to 210%, while the growth rate was decreased under nitrogen deficient condition. The contents of C16:0 and C16:1 increased dramatically in both N. oculata K-1281 and LB2164, while the contents of C20:4 and C20:5 increased in N. oculata LB2164. The fatty acids content and composition in N. oculata K-1281 returned following addition of nitrogen after nitrogen starvation. These results demonstrated that fatty acid contents and compositions under nitrogen deficiency will provide the understanding of fatty acid synthesis in microalgae.

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