Korean Circulation Journal

The Korean Society of Cardiology (대한심장학회)
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Glucose-Insulin-Potassium Solution Protects Ventricular Myocytes of Neonatal Rat in an In Vitro Coverslip Ischemia/Reperfusion Model

  • Chun, Woo-Jung (Division of Cardiology, Department of Internal Medicine, Samsung Changwon Hospital, Sungkyunkwan University, College of Medicine) ;
  • Nah, Deuk-Young (Division of Cardiology, Department of Internal Medicine, College of Medicine, Dongguk University) ;
  • Bae, Jun-Ho (Division of Cardiology, Department of Internal Medicine, College of Medicine, Dongguk University) ;
  • Chung, Jin-Wook (Division of Cardiology, Department of Internal Medicine, College of Medicine, Dongguk University) ;
  • Lee, HyunSook (Department of Anatomy, Dongguk Medical Institute, College of Medicine, Dongguk University) ;
  • Moon, Il Soo (Department of Anatomy, Dongguk Medical Institute, College of Medicine, Dongguk University)
  • Received : 2013.07.02
  • Accepted : 2015.03.09
  • Published : 2015.05.30
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Abstract

Background and Objectives: The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury. Materials and Methods: NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model. Results: Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression. Conclusion: The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation.

Keywords

Acknowledgement

Supported by : The Korean Society of Cardiology

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