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http://dx.doi.org/10.4070/kcj.2015.45.3.234

Glucose-Insulin-Potassium Solution Protects Ventricular Myocytes of Neonatal Rat in an In Vitro Coverslip Ischemia/Reperfusion Model  

Chun, Woo-Jung (Division of Cardiology, Department of Internal Medicine, Samsung Changwon Hospital, Sungkyunkwan University, College of Medicine)
Nah, Deuk-Young (Division of Cardiology, Department of Internal Medicine, College of Medicine, Dongguk University)
Bae, Jun-Ho (Division of Cardiology, Department of Internal Medicine, College of Medicine, Dongguk University)
Chung, Jin-Wook (Division of Cardiology, Department of Internal Medicine, College of Medicine, Dongguk University)
Lee, HyunSook (Department of Anatomy, Dongguk Medical Institute, College of Medicine, Dongguk University)
Moon, Il Soo (Department of Anatomy, Dongguk Medical Institute, College of Medicine, Dongguk University)
Publication Information
Korean Circulation Journal / v.45, no.3, 2015 , pp. 234-241 More about this Journal
Abstract
Background and Objectives: The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury. Materials and Methods: NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model. Results: Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression. Conclusion: The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation.
Keywords
Myocytes, cardiac; Glucose-insulin-potassium cardioplegic solution; GlcNAc; O -GlcNac transferase;
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