An outbreak of Lactococcus garvieae Infection in Cage-cultured Red Lip Mullet Chelon haematocheilus with Green Liver Syndrome

Abstract

Red lip mullet Chelon haematocheilus (body weight = $468{\pm}91g$) which became sick during an outbreak of disease at mariculture facilities at Cheonsu Bay, Korea, during July-August 2013, were examined to identify the cause of the disease. Diseased mullets displayed green liver syndrome, and Lactococcus garvieae were isolated from their internal organs. Argulus sp., Trichodina sp., and/or Vibrio spp. were also discovered in some infected fish. Histopathological examination revealed that fatty liver syndrome with hepatocyte degeneration, reflected in heterokaryons, inflammatory lesions, and melanomacrophage centers ($MMC_S$), had caused fibrosis around the kidney, spleen, and blood vessels. After the outbreak, visceral fat and green liver syndrome in the mullets were consistently observed throughout the year in the same mariculture facilities, indicating that the cultured mullets suffered a chronic metabolic disorder. Although Vibrio spp. were also isolated from some individuals, L. garvieae, which is known to be a causative agent of red lip mullet mortality, was isolated from all diseased individuals. This is the first report of L. garvieae infection in cultured red lip mullet.

Introduction

Coastal mullets are the most widely distributed of the bony fishes in both the temperate and tropical regions of the Pacific and Atlantic oceans. Grey mullet Mugil cephalus and red lip mullet Chelon haematocheilus are distributed along the entire coast of Korea. Mullets have been caught frequently from the Yeongsan River, Hwaseong, Gyeonggi-do, and Ganghwa regions, and their consumption has increased despite the reduced catch in recent years due to overhunting and reclamation (Jang, 2002). Mullets are cultivated to meet the demand for consumption and accounted for 8% of the total amount of cultivated fish in Korea in 2012, with olive flounder Paralichthys olivaceus and black rockfish Sebastes schlegeli being the next most widely cultivated species (Ministry of Maritime Affairs and Fisheries, 2012). Red lip mullet, one of the most valuable species in the Korean aquaculture industry, is mainly cultured along the west coast of Korea. Mullets have a high mortality rate during cultivation, but the reasons for this are currently unknown. There is little research regarding mullet disease in Korea, with only Myxobolus sp. and Amyloodinium sp. infections reported so far (Park et al., 2006; Kim et al., 2013).

Green liver syndrome refers to macroscopic observations of fish liver having a dark- or light-green color rather than its normal light brown appearance. Green liver syndrome is frequently observed in red sea bream Pagrus major during periods of low water temperature in Korea, and it is known to be caused by the physiological effects of exposure to low temperatures (National Fisheries Research and Development Institute, 2012). Green liver syndrome in fish also occurs due to the adverse effects of poor quality feed, including feeds lacking essential proteins such as taurine (Goto et al., 2001; Takagi et al., 2010).

During July–August 2013, mortalities were noted in cultured red lip mullet stocks in Cheonsu Bay on the west coast of Korea. The dead fish displayed abdominal distension and green liver syndrome. We undertook an investigation to identify the cause of the disease.

 

Materials and Methods

Disease outbreak and fish sampling

Mass mortality of red lip mullets displaying abdominal distension and green liver syndrome, was recorded at three fish farms (Fig. 1; Farms A, B and C) in Cheonsu Bay, Chungcheongnam-do, from late July until mid-August 2013. At farms A and C, where approximately 100,000 red lip mullet were cultivated, an average of 100–150 fish died over 15 consecutive days. The cumulative mortality accounted for 2.1% of the total fish stock (average fish body weight farm A = 384 ± 109 g, farm C = 498 ± 70 g). At farm B, where approximately 100,000 red lip mullet were cultivated, an average of 200–250 fish died over 15 consecutive days. The cumulative mortality accounted for 3.5% of the total fish stock (average fish body weight 523 ± 10 g). Three moribund red lip mullet from each farm were used in this study.

Fig. 1.Map of sampling sites in Cheonsu Bay (a) Dangamri, (b) Changri, (c) Ganwaldo.

Parasite and virus examination

Parasite infection was examined using a speculum to obtain biopsy samples comprising the gills, body surface, fin slime, and internal organs (brain, kidney, and spleen) of red lip mullet. Marine birnavirus (MBV; MBV-F: 5′-GCACCACGAAGGTACGAAAT-3′, MBV-R: 5′-GTACGTTGCCGTTTCCTGAT-3′), viral hemorrhagic septicemia virus (VHSV; VHSV-F: F: 5′-ATGGAAGGAGGAATTCGTGAAGCG-3′, VHSV-R: R: 5′-GCGGTGAAGTGCTGCAGTTCC-C-3′), hirame rhabdovirus (HRV; HRV-F: F: 5′-TGAGGTAGAGCAAGCCAACA-3′, HRV-R: R: 5′-ACATGACTGGCCGAAAAGAG-3′), viral nervous necrosis virus (VNNV; VNNV-F: F: 5′-CGTGTCAGTCATGTGTCGCT-3′, VNNV-R: R: 5′-CGAGTCAACACGGGTGAAGA-3′), and red seabream iridovirus (RSIV; RSIV-F: F: 5′-CTCAAACACTCTGGCTCATC-3′, RSIV-R: R: 5′-GCACCAACACATCTCCTATC-3′) were detected by polymerase chain reaction (PCR). Internal organs including the kidney and spleen were removed from each fish. DNA for PCR was isolated using a High Pure PCR Template Preparation Kit (Roche, Basel, Switzerland), RNA was isolated using TRIZOL® Reagent (Invitrogen, Carlsbad, CA, USA) according to the manual, and cDNA was produced using SuperScriptTMII Reverse Transcriptase (Invitrogen, USA). The isolated DNA was applied as a template for the analysis of RSIV PCR, and the synthesized cDNA was used for the analysis of VHSV, VNNV, HRV, and MBV PCR. PCR was conducted using ExTaq® (Takara, Shiga, Japan). The amplified products were verified by 1.5% agarose gel electrophoresis (Bioneer, Daejeon, Korea), and all PCR analyses were compared through positive and negative contrasts.

Bacterial examination

The liver, kidney, and spleen of moribund fish were inoculated onto brain heart infusion agar (BHIA; Difco, Sparks, MD, USA) containing 1% (w/v) sodium chloride (BNA), and incubated at 25°C for 24-48 hours. Hemolytic activity was determined by 5% sheep blood agar medium (Micro Media Co., Ltd, Seoul, Korea) at 25°C for 48 hours.

(a) Biochemical analysis

All strains were tested for biochemical characteristics using the API 20E and API strep systems (BioMerieux, Marcy l’Etoile, France) according to the manufacturer’s protocols. The oxidase test consisted of a cytochrome oxidase paper test and catalase slide test using 3% H2O2.

(b) 16S rRNA gene sequencing

Bacterial genomic DNA was isolated using the High Pure PCR Template Preparation Kit and ExTaq® following the manufacturer’s protocol. The DNA templates were amplified using PCR in a Gene Amp PCR System 2400 (Perkin Elmer; Wellesley, MA, USA) using universal primers amplifying a 1343-bp region of the 16S rRNA gene (63F: 5′-CAGGCCTAACACATGCAAGTC-3′; 1406R: 5′-ACGGGCGGTGTGTRC-3′) obtained from Bioneer. The DNA templates were amplified by initial denaturation at 94°C for 5 min, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, extending at 72°C for 1.5 min, and a final extension at 72°C for 5 min. Direct sequencing of the amplified DNA fragment was performed using a 3730xl DNA automatic sequencer (Applied Biosystems; Foster City, CA, USA). All sequences were submitted for similarity searches with BLAST (Altschul et al., 1990).

Histopathology

The tissues collected from each individual were fixed in 10% neutral buffered formalin (NBF). After fixation, standard histological procedures were used for tissue dehydration and paraffin embedding. Tissue sections were stained with hematoxylin–eosin (H&E), and the tissue specimens were observed under an optical microscope (Zeiss, Jena, Germany).

 

Results

Clinical signs

All red lip mullet investigated at the three fish farms displayed abdominal distension, and some had petechial hemorrhages and congestion or hemorrhages at the base of the fins as external symptoms (Fig. 2A). Ascites and green liver syndrome appeared as internal symptoms (Fig. 2B, 2C).

Fig. 2.(A) DA, distended abdomen; (B) AF, ascitic fluid in abdominal cavity; (C) GL, green liver symptom; VF, visceral fat.

Parasite and virus examination

Argulus sp. or Trichodina sp. infection was identified using a speculum to obtain biopsy samples consisting of gills and body surface of some red lip mullet specimens (Table 1). No virus was detected from any red lip mullet inspected by RSIV, VHSV, VNNV, HRV, and MBV PCR analysis (data not shown).

Table 1.Diagnostic results of this study

Bacterial examination

The prevailing milky white colonies (ML-01, ML-02, and ML-03) were grown on BNA smeared with kidney and spleen samples from all fish (n = 9), and glossy colonies (MV-01, MV-02, and MV-03) were also isolated from some fish (n = 3) (Table 1). The 16S rRNA gene sequences of the prevailing isolates (ML-01, ML-02, and ML-03) were 100% matched with Lactococcus garvieae ATCC49156 strains isolated from cultured Japanese yellowtail in 1974 (GenBank accession no. NR 102968.1) (Table 2). These three strains, with identical 16S RNA gene attributes, exhibited differences in acid production from mannitol and glucose according to test strains (Table 2). The 16S rRNA gene sequence of MV-01 were most closely related (99.9% identity) to the sequence of Vibrio vulnificus (GenBank accession no. KC748469.1). However, the 16S rRNA gene sequences of MV-02 and MV-03 had 99.9% identity with the sequence of V. harveyi (GenBank accession no. KC884631.1) (Table 2).

Table 2.+, positive reaction; -, negative reaction; ND, not done

Histopathology

Histopathological examination revealed apparent fatty liver changes, with hepatocyte degeneration apparent as heterokaryons or fissile materials and the cytoplasm of degenerated cells containing cell debris. The number and size of fat droplets (i.e., white vacuoles) varied, and the size, shape, and arrangement of hepatocytes were irregular (Fig. 3A, 3B). Degenerated cells were surrounded by epithelioid cells, which is the initial stage of tuberculation (Fig. 3B). Inflammatory degeneration, melanomacrophage centers (MMCS), and fibrosis were identified around blood vessels (Fig. 3A). MMCs in various sizes and an increase in degenerated macrophages containing phagocytosed substances were observed in the spleen (Fig. 3C). An aggregation of macrophages with brown pigmentation was occasionally observed in the head kidney (Fig. 3D), and weak eosinophilic degeneration was sometimes noticed in the epithelium of renal tubules (data not shown). Inflammatory cell infiltration was observed in serous membranes and fat tissue around the heart and in the connective tissue layer of the digestive tract (Fig. 3E, 3F).

Fig. 3.(A) Liver: fi: fibrosis around vein, a: aorta, s: sinus, MMCs: melanomacrophage centers, V: vein, circle: around vein showing inflammatory degeneration, (B) Liver: arrows: eosinophilic degenerated cells, circles: early stage of granuloma surrounded with epitheloid cells, v:vein, (C) Spleen: MMCs: melanomacrophage centers, arrows: larged and degenerated macrophages, (D) Head kidney: circles: aggregate of pigmented cells, arrows: large and degenerated macrophages, (E) Heart: inflammatory cells infiltration at heart serosa, a:aorta, Ft:fat tissue, sv:sinus venosus, v:vein, (F) Stomach: inflammatory cells infiltration at layers from LP to SM, LP:lamina propria, ML: muscular layer, SM: serouse membrane.

 

Discussion

Although Vibrio spp. were also isolated from some individuals, L. garvieae, which is known be a causative agent of red lip mullet mortality, was isolated in all diseased mullet specimens. L. garvieae has been isolated as a causative agent of disease in both freshwater and seawater fishes, such as rainbow trout Oncorhynchus mykiss, yellowtail Seriola quinqueradiata, tilapia Oreochromis sp., eel Anguilla japonica, olive flounder P. olivaceus, grey mullet, and black rockfish S. schlegeli (Vendrell et al., 2006). L. garvieae was isolated as a pathogen from olive flounder, black rockfish, and yellowtail in Korea (Lee et al., 2001). Although L. garvieae has been isolated from diseased grey mullet, basket mullet Chelone atatus, and Borneo mullet Chelone macrolepis (Tsai et al., 2012), this is the first report of its presence in cultured red lip mullet C. haematocheilus. Chen et al. (2002) reported that more than 10% of cultivated grey mullets showed mortality from infection by L. garvieae between 1999 and 2000 in Taiwan. Hypertrophy of kidney and spleen, small white spots on internal organs, and a distended abdomen with yellow ascites were the major symptoms in mullet infected with L. garviae (Chen et al., 2002). We also found hypertrophy of the kidney and spleen and abdominal distension due to ascites in cultured moribund red lip mullet; thus, these characteristic symptoms seem to be related to infection by L. garvieae. Unlike the reports from Taiwan, small white spots were not found in the organs of diseased red lip mullet from Cheonsu Bay, and green liver syndrome was observed in all mullet specimens.

All red lip mullet specimens showed visceral fat with green liver syndrome as internal symptoms, regardless of the fish farm investigated (Fig. 2C). The visceral fat and green liver syndrome were observed consistently throughout year at all three farms (data not shown). A fat metabolism problem was also identified, as the distribution of fat cells in the liver was histopathologically irregular. The greenish-brown liver tissue likely had local cholestasis, with a fat metabolite such as lipofucsin producing a bile duct obstruction. In previous studies, green liver syndrome in grey mullet cultivated in Korea was observed to persist throughout the year (Lee, 2008), as was also found in this study. Green liver syndrome in grey mullet is related to nutrition hypertrophy in hepatocytes due to excessive nutrient absorption, suggesting that feeding suspension might improve green liver syndrome (Lee, 2008). The cause of green liver syndrome in the diseased mullets was estimated to be hypernutrition, with the use of low quality assorted feeds that are currently available in Korea also having a negative influence on mullet health. A nutritional imbalance may be created when the feedstuff lacks essential proteins such as taurine. The mullet feed available in the market in Korea is cheaper than the assorted feed used for other seawater fishes, which consists of a low-fishmeal diet (less than 30%) and high soy protein concentrate (Jeil Feed Co., Ltd, personal communication). Fishmeal is a finite resource, with limited availability and high demand, and it is the most expensive protein ingredient in fish diets. It is necessary to investigate any correlations between feed ingredients, the amount provided, and the incidence of disease, as the feedstuffs provided at all three of the fish farms where disease occurred were available in the local Korean market.

It is also likely that the mass mortalities of red lip mullet in Cheonsu Bay were related to changes in water temperature. The temperature appeared to rise sharply from 22°C in mid-July to 26-28°C in late July through early August (http;//egov.nfrdi.re.kr-Comprehensive Status Information). This period of rising water temperature is consistent with the mass mortality period. Lactococcus garvieae is less virulent at low temperatures, and it is known to cause disease in summer at temperatures over 16°C (Vendrell et al., 2006). Stress due to a rise in water temperature is therefore considered to affect L. garvieae infection.