농약과학회지 (The Korean Journal of Pesticide Science)

  • 제19권3호
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  • Pages.301-311
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  • 2015
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
권호 표지
DOI QR코드

DOI QR Code

혼합 비티 독소단백질과 아이코사노이드 생합성 억제자를 이용한 약효 증진 기술

A Technique to Enhance Insecticidal Efficacy Using Bt Cry Toxin Mixture and Eicosanoid Biosynthesis Inhibitor

  • 엄성현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박영진 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Eom, Seonghyeon (Department of Bioresource Sciences, Andong National University) ;
  • Park, Youngjin (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Yonggyun (Department of Bioresource Sciences, Andong National University)
  • 투고 : 2015.06.22
  • 심사 : 2015.08.11
  • 발행 : 2015.09.30
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초록

Bacillus thuringiensis (비티)의 약효를 증가시키기 위한 일환으로 Cry 독소단백질의 혼합효과를 검정하였다. 서로 다른 네 가지 비티 균주에서 분리된 Cry 독소단백질 추출물들은 각각 좁은 적용해충범위를 나타냈다. 이들 Cry 독소단백질을 혼합한 결과 적용범위가 현격하게 증가했다. Xenorhabdus nematophila (Xn) 세균 배양액은 조사된 모든 곤충의 세포성 면역을 억제하고 Cry 독소단백질의 살충력을 증가시켰다. 이 Xn 세균배양액을 혼합 Cry 독소단백질에 추가한 결과 적용해충범위와 살충력을 모두 증가시켰다.

To enhance Bacillus thuringiensis (Bt) efficacy, four Cry toxins were purified from four different Bt strains and assessed in their combined efficacy. The Cry mixtures significantly expanded their target insect spectra. Bacterial culture broth of Xenorhabdus nematophila (Xn) significantly suppressed insect cellular immune response and increased Cry toxicity. The addition of Xn culture broth to Cry mixture significantly enhanced Bt efficacy in target insect spectrum and insecticidal activity.

키워드

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