Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Selenium, Vitamin E, and Their Combination on Growth, Hematological Changes, and Biological Blood Parameters in Orchidectomized Rat Model

Vitamin E와 Selenium이 정소적출 포유동물모델의 성장, 혈액 및 생화학적인 변화에 미치는 영향

  • Kim, Hyun (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Choe, Changyong (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 김현 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 최창용 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Received : 2015.08.12
  • Accepted : 2015.08.23
  • Published : 2015.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was devised to determine the effects of vitamin E and selenium (Selevit) on body weight, organ weight, hematological values and biochemical parameters in the orchidectomized (Orch) rats. Intact group (n=15) received no treatment and operation. Orch+Selevit received operation and Selevit. The body weights of each group increased, but that of the Orch＋Selevit group were significantly lower than those of all the other groups. There were significantly different decreased (p<0.001) of body weights between Orch＋Selevit group and all the other groups. Also, organ weights such as heart, liver, spleen, kidney, lung and skeletal muscle were measured. The heart and liver weights in the Orch＋Selevit group were significantly different decreased (p<0.001) in comparison with those in the Intact and Sham groups. The kidney weights in the Orch＋Selevit group were significantly different decreased (p<0.01, p<0.001) in comparison with those in all the other groups. The number of white blood cell (WBC) was significantly higher (p<0.05) in the Orch＋Selevit group than in all the other groups. The hematological values of 12 parameters were not significantly different in any of the groups. The concentrations of serum total protein, albumin and alkaline phosphatase only increased significantly (p<0.05, p<0.01) in the Orch＋Selevit group as compared to that in the Orch group. We conclude that Selevit was significantly decreased the body weight in the Orch rats. Our findings suggest that Selevit may influence the process of lipid packaging and absorption in the Orch rats.

이 연구에서는 정소가 제거된 흰쥐에 비타민 E와 셀레늄(Selevit)을 5주간 투여 후 체중, 장기 무게, 혈액학적 그리고 생화학적 변화를 관찰하였다. 체중의 변화에서는 모든 실험군에서 증가가 나타냈다. Orch+Selevit군의 체중 증가는 $11.2{\pm}10.25g$으로 가장 낮았으며, Intact군, Sham군, Orch군과 비교 시 유의적으로 감소되었다. 장기 무게의 변화에서는 Orch+Selevit군의 심장과 간장 무게는 Intact군, Sham군과 비교 시 유의적으로 감소되었다. Intact군, Sham군, Orch군의 신장 무게는 Orch+Selevit군 비교 시 유의적인 증가를 확인하였다. 백혈구 수의 혈액학적 변화에서는 Orch+Selevit군은 다른 모든 군과 비교해 유의적인 증가를 확인하였으며, 적혈구수, 평균적혈구용적, 평균적혈구혈색소량, 평균적혈구색소농도 등과 같은 혈액학적 측정치에서는 유의성은 인정되지 않았다. 생화학적 변화에서는 Orch+Selevit군의 혈청총단백질, 알부민은 Orch군과 비교 시 유의적으로 증가하였으며, 알부민은 Intact군, Sham군 그리고 Orch군과 비교 시 유의적으로 감소했다. AST와 ALT는 모든 실험군에서 유의성이 없었다.

Keywords

References

  1. Al Shamsi MS, Amin A, Adeghate E (2004) Beneficial effect of vitamin E on the metabolic parameters of diabetic rats. Mol Cell Biochem 261:35-42. https://doi.org/10.1023/B:MCBI.0000028735.79172.9b
  2. Akcam M (2010) Helicobacter pylori and micronutrients. Indian Pediatr 47:119-126. https://doi.org/10.1007/s13312-010-0017-2
  3. Awad JA, Morrow JD, Hill KE, Roberts LJ, Burk RF (1994) Detection and localization of lipid peroxidation in selenium and vitamin E-deficient rats using F2-isoprostanes. J Nutr 124:810-816. https://doi.org/10.1093/jn/124.6.810
  4. Beck MA, Matthews CC (2000) Micronutrients and host resistance to viral infection. Proc Nutr Soc 59:581-585. https://doi.org/10.1017/S0029665100000823
  5. Brigelius-Flohe R, Kelly FJ, Salonen JT, Neuzil J, Zingg JM, Azzi A (2002) The European perspective on vitamin E: current knowledge and future research. Am J Clin Nutr 76:703-716. https://doi.org/10.1093/ajcn/76.4.703
  6. Burton GW, Ingold KU, Cheeseman KH, Slater TF (1990) Application of deuterated alpha tocopherols to the biokinetics and bioavailability of vitamin E. Free Radic Res Commun 11:99-107. https://doi.org/10.3109/10715769009109672
  7. Combs GF (2005) Current evidence and research needs to support a health claim for selenium and cancer prevention. J Nutr 135:343-347. https://doi.org/10.1093/jn/135.2.343
  8. Coquette A, Vray B, Vanderpas J (1986) Role of vitamin E in the protection of the resident macrophage membrane against oxidative damage. Arch Int Physiol Biochim 94:29-34.
  9. Esterbauer H, Dieber-Rotheneder M, Striegl G, Waeg G (1991) Role of vitamin E in preventing the oxidation of low-density lipoprotein. Am J Clin Nutr 53:314S-321S. https://doi.org/10.1093/ajcn/53.1.314S
  10. Hori K, Hatfield D, Maldarelli F, Lee BJ, Clouse KA (1997) Selenium supplementation suppresses tumor necrosis factor alpha-induced human immunodeficiency virus type 1 replication in vitro. AIDS Res Hum Retroviruses 13:1325-1332. https://doi.org/10.1089/aid.1997.13.1325
  11. Jansson L, Lindroth M, Tyopponen J (1984) Intestinal absorption of vitamin E in low birth weight infants. Acta Paediatr Scand 73:329-332. https://doi.org/10.1111/j.1651-2227.1994.tb17743.x
  12. Jeanes YM, Hall WL, Ellard S, Lee E, Lodge JK (2004) The absorption of vitamin E is influenced by the amount of fat in a meal and the food matrix. Br J Nutr 92:575-579. https://doi.org/10.1079/BJN20041249
  13. Katzen-Luchenta J (2007) The declaration of nutrition, health, and intelligence for the child-to-be. Nutr Health 19:85-102. https://doi.org/10.1177/026010600701900212
  14. Kayden HJ (2001) The genetic basis of vitamin E deficiency in humans. Nutrition 17:797-798. https://doi.org/10.1016/S0899-9007(01)00650-5
  15. Kiremidjian-Schumacher L, Roy M, Wishe HI, Cohen MW, Stotzky G (1994) Supplementation with selenium and human immune cell functions. II. Effect on cytotoxic lymphocytes and natural killer cells. Biol Trace Elem Res 41:115-127. https://doi.org/10.1007/BF02917222
  16. Longtin R (2003) Selenium for prevention: Eating your way to better DNA repair? J Natl Cancer Inst 95: 98-100. https://doi.org/10.1093/jnci/95.2.98
  17. Moriguchi S, Muraga M (2000) Vitamin E and immunity. Vitam Horm 59:305-336. https://doi.org/10.1016/S0083-6729(00)59011-6
  18. Molnar J, MacPherson A, Barclay I, Molnar P (1995) Selenium content of convenience and fast foods in Ayrshire, Scotland. Int J Food Sci Nutr 46:343-352. https://doi.org/10.3109/09637489509012566
  19. Rayman MP, Infante HG, Sargent M (2008) Foodchain selenium and human health: Spotlight on speciation. Br J Nutr 100:238-253.
  20. Roxborough HE, Burton GW, Kelly FJ (2000) Interand intraindividual variation in plasma and red blood cell vitamin E after supplementation. Free Radic Res 33:437-445. https://doi.org/10.1080/10715760000300971
  21. Sakaguchi S, Iizuka Y, Furusawa S, Tanaka Y, Takayanagi M, Takayanagi Y (2000) Roles of selenium in endotoxininduced lipid peroxidation in the rats liver and in nitric oxide production in J774A. Toxicol Lett 118:69-77. https://doi.org/10.1016/S0378-4274(00)00263-0
  22. Schrauzer, GN (2003) The nutritional significance, metabolism and toxicology of selenomethionine. Adv Food Nutr Res 47:73-112. https://doi.org/10.1016/S1043-4526(03)47002-2
  23. Schwarz K, Foltz CM (1999) Selenium as an integral part of factor 3 against dietary necrotic liver degeneration. Nutrition 15:255.
  24. Sies H, Stahl W, Sundquist AR (1992) Antioxidant functions of vitamins. Vitamins E and C, beta-carotene, and other carotenoids. Ann N Y Acad Sci 669:7-20. https://doi.org/10.1111/j.1749-6632.1992.tb17085.x
  25. Thomas DR (2006) Vitamins in aging, health, and longevity. Clin Interv Aging 1:81-91. https://doi.org/10.2147/ciia.2006.1.1.81
  26. Wright MO, Burden V, Lee J, Eitenmiller RR, Fischer JG (1999) High dietary iron enhances oxidative stress in liver but does not increase aberrant crypt foci development in rats with low vitamin E status. Nutr Cancer 35:143-152. https://doi.org/10.1207/S15327914NC352_8
  27. Yachi R, Igarashi O, Kiyose C (2010) Protective effects of vitamin E analogs against carbon tetrachlorideinduced fatty liver in rats. J Clin Biochem Nutr 47: 148-154. https://doi.org/10.3164/jcbn.10-35