한국어병학회지 (Journal of fish pathology)

  • 제28권2호
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  • Pages.99-107
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  • 2015
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지
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Genomic identification and spatial expression analysis of Rab-5C-like gene identified from rock bream(Oplegnathus fasciatus)

  • Mothishri, M.S. (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Umasuthan, Navaneethaiyer (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Thulasitha, William Shanthakumar (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Whang, Ilson (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University) ;
  • Lee, Jehee (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
  • 투고 : 2014.11.26
  • 심사 : 2015.07.23
  • 발행 : 2015.08.30
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초록

Despite its economic importance as an aquaculture species, the molecular and genetic information regarding physiologically important elements in rock bream (Oplegnathus fasciatus) is not completely understood. Rab proteins play a vital role in cellular mechanisms and immunity as one of the key regulators of membrane trafficking. In this investigation, a Rab gene, named as RbRab-5C-like, was identified from Oplegnathus fasciatus. RbRab-5C-like protein exhibited high homology with Rab proteins of other species and possessed signature characteristics of Rab proteins with four conserved cysteine residues. Phylogenetic analysis showed that RbRab-5C-like clustered with other fish counterparts. The RbRab-5C-like genomic sequence possesses six exons and five introns. Transcriptional analysis revealed that RbRab-5C-like was ubiquitously expressed in all examined tissues with the highest expression occurring in the liver. While the structural and homologic characteristics of RbRab-5C-like suggest a strong conservation of this element in different species, its mRNA distribution implies a wide range of biological significance in rock bream.

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참고문헌

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