Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-atopic Effect of Hot Water and Supercritical Carbon Dioxide Fluid Extract of Persimmon (Diospyros kaki) Peels

감껍질 열수 및 초임계 유체 추출물의 항아토피 효과

  • Received : 2015.03.13
  • Accepted : 2015.04.10
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study aimed to investigate the anti-atopic effect of hot water (PPWE) and supercritical-carbon dioxide fluid extract of persimmon peels (SPPE) on atopic dermatitis (AD)-like skin lesions in hairless mice. Histological analyses demonstrated that SPPE treatment more strongly inhibited the dermal infiltration of inflammatory cells in AD-like skin lesions than that by PPWE. Compared to PPWE, SPPE significantly decreased the dermatitis clinical score and the epidermal thickness and potently suppressed serum IgE and interleukin (IL)-4 production in hairless mice with AD. Furthermore, compared to PPWE, SPPE potently inhibited the production of nitric oxide, prostaglandin $E_2$, and proinflammatory cytokines such as IL-6 and IL-$1{\beta}$ in lipopolysaccharide-stimulated RAW264.7 macrophages. These results suggested that SPPE exhibited anti-atopic dermatitis activity via the regulation of inflammatory responses.

본 연구에서는 고종시 감껍질을 열수 추출 및 초임계 유체 추출하여 아토피 피부염 증상 억제 효과를 밝히고, 항염 효능을 나타내는 기능성 소재로서의 이용 가능성을 알아보고자 하였다. 그 결과 육안 평가를 통해 피부의 홍반(erythema), 가려움과 피부의 건조상태(pruritus and dry skin), 부종과 혈종(edema and excoriation), 짓무름(erosion), 그리고 태선화(lichenification)와 같은 아토피 피부염 같은 증상이 AD 모델에서 증가하였지만, SPPE와 PPWE를 투여하였을 경우 완화되는 것을 확인할 수 있었으며, SPPE가 PPWE보다 더 뛰어난 효과를 나타내었다. 피부 두께와 염증 세포의 침윤은 AD 모델에서 크게 증가하였지만, SPPE와 PPWE를 투여하였을 경우 감소하는 것을 확인할 수 있었으며, SPPE가 PPWE보다 더 뛰어난 효과를 나타내었다. 혈청 중의 IgE와 IL-4의 수치를 측정한 결과, AD 모델에서 크게 증가하였으나 SPPE와 PPWE를 투여하였을 경우 감소하는 것을 확인할 수 있었으며, SPPE가 PPWE보다 더 뛰어나게 억제하는 효과를 나타내었다. 또한 RAW264.7 세포에 SPPE를 처리하였을 경우 염증 매개 인자인 NO, $PGE_2$, IL-6, IL-$1{\beta}$의 생성량이 유의적의로 감소하였고, PPWE의 경우 NO, $PGE_2$, IL-$1{\beta}$의 생성을 억제한 반면 IL-6 생성 억제에는 영향을 나타내지 않았다. 이러한 염증 매개 인자 억제 효능은 SPPE가 PPWE보다 더 뛰어나게 억제하는 것을 확인하였다. 따라서 감껍질 추출물은 아토피 피부염 증상 개선과 염증관련 질환 치료를 위한 기능성 천연물 소재로 유용하게 활용될 수 있을 것으로 판단된다.

Keywords

References

  1. Shaw TE, Currie GP, Koudelka CW, Simpson EL. Eczema prevalence in the United States: Data from the 2003 national survey of children's health. J. Invest. Dermatol. 131: 67-73 (2011) https://doi.org/10.1038/jid.2010.251
  2. Guttman-Yassky E, Nograles KE, Krueger JG. Contrasting pathogenesis of atopic dermatitis and psoriasis-part I: Clinical and pathologic concepts. J. Allergy Clin. Immun. 127: 1110-1118 (2011) https://doi.org/10.1016/j.jaci.2011.01.053
  3. Kim BJ, Son WR, Choi MO, Jo SK, Jung HK, Lee JT, Kim HY, Kwoen DJ. Anti-atopic effects of Castanea crenata inner shell extracts fermented by Lactobacillus bifermentans. J. Korean Soc. Food Sci. Nutr. 42: 1378-1386 (2013) https://doi.org/10.3746/jkfn.2013.42.9.1378
  4. Boguniewicz M, Leung DYM. Recent insights into atopic dermatitis and implications for management of infectious complications. J. Allergy Clin. Immun. 125: 4-13 (2010) https://doi.org/10.1016/j.jaci.2009.11.027
  5. Leung DYM. Pathogenesis of atopic dermatitis. J. Allergy Clin. Immun. 104: S99-S108 (1999) https://doi.org/10.1016/S0091-6749(99)70051-5
  6. Cha WS, Yoo JH, Min MJ, Nam HG, Kim YS, Shin HJ, Kim JS, Choi OY, Kim R, Choi DB. Effect of complex extracts of mushroom and sharp toothed eel on the skin conditions with atopic dermatitis and acne symptoms. Korea Soc. Biotechnol. Bioeng. J. 25: 387-394 (2010)
  7. Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Kim BR, Ahn NK, Choi YU, Bae NY, Park JH, Ahn DH. Anti-atopic activity of tuna heart ethanol extract. J. Korean Soc. Food Sci. Nutr. 44: 1-6 (2015) https://doi.org/10.3746/jkfn.2015.44.1.001
  8. Joo YH, Won CH, Kim JY, Cho KH, Min KU, Kim KH. Developing an atopic dermatitis model and the effects of Actinidia extract on dermatitis in NC/Nga mice. Korean J. Dermatol. 47: 1105-1112 (2009)
  9. Choi MJ, Jung HK, Jeong YS, Park SC, Hong JH. Anti-allergic activities of fermented Eriobotrya japonica and Saurus chinensis extracts in 2,4-dinitrochlorobezene-induced BALB/c mice. J. Korean Soc. Food Sci. Nutr. 39: 1611-1618 (2010) https://doi.org/10.3746/jkfn.2010.39.11.1611
  10. Cho SE. A study on the immunomodulatory effects of Chamaecyparis obtusa leaves on NC/Nga mice as models for atopic dermatitis. J. Korean Soc. Cosmetol. 18: 78-89 (2012)
  11. Chen XN, Fan JF, Yue X, Wu XR, Li LT. Radical scavenging activity and phenolic compounds in persimmon (Diospyros kaki L. cv. Mopan). J. Food Sci. 73: C24-C28 (2008)
  12. Jeong SI, Cho JK, Mok JY, Kim SJ, Park JM, Jeon IH, Kim HS, Jang SI. Antioxidant activity of persimmon leaves during growth. Kor. J. Pharmacogn. 41: 255-263 (2010)
  13. Chung JY, Kim KH, Shin DJ, Son GM. Effects of sweet persimmon powder on the characteristics of bread. J. Korean Soc. Food Sci. Nutr. 31: 738-742 (2002) https://doi.org/10.3746/jkfn.2002.31.5.738
  14. Seo JH, Jeong YJ, Kim KS. Physiological characteristics of tannins isolated from astringent persimmon fruits. Korean J. Food Sci. Technol. 32: 212-217 (2000)
  15. Kawakami K, Aketa S, Nakanami M, Iizuka S, Hirayama M. Major water-soluble polyphenols, proanthocyanidins, in leaves of persimmon (Diospyros kaki) and their ${\alpha}$-amylase inhibitory activity. Biosci. Biotech. Bioch. 74: 1380-1385 (2010) https://doi.org/10.1271/bbb.100056
  16. Fukai S, Tanimoto S, Maeda A, Fukuda H, Okada Y, Nomura M. Pharmacological activity of compounds extracted from persimmon peel (Diospyros kaki THUNB.). J. Oleo Sci. 58: 213-219 (2009) https://doi.org/10.5650/jos.58.213
  17. Kim SY, Jeong SM, Kim SJ, Jeon KI, Park EJ, Park HR, Lee SC. Effect of heat treatment on the antioxidative and antigenotoxic activity of extracts from persimmon (Diospyros kaki L.) peel. Biosci. Biotech. Bioch. 70: 999-1002 (2006) https://doi.org/10.1271/bbb.70.999
  18. Son JE, Hwang MK, Lee EJ, Seo SG, Kim JE, Jung SK, Kim JR, Ahn GH, Lee KW, Lee HJ. Persimmon peel extract attenuates PDGF-BB-induced human aortic smooth muscle cell migration and invasion through inhibition of c-Src activity. Food Chem. 141: 3309-3316 (2013) https://doi.org/10.1016/j.foodchem.2013.06.038
  19. Lee YA, Cho EJ, Tanaka T, Yokozawa T. Inhibitory activities of proanthocyanidins from persimmon against oxidative stress and digestive enzymes related to diabetes. J. Nutr. Sci. Vitaminol. 53: 287-292 (2007) https://doi.org/10.3177/jnsv.53.287
  20. Lee YA, Kim YJ, Cho EJ, Yokozawa T. Ameliorative effects of proanthocyanidin on oxidative stress and inflammation in streptozotocin- induced diabetic rats. J. Agr. Food Chem. 55: 9395-9400 (2007) https://doi.org/10.1021/jf071523u
  21. Lee YA, Cho EJ, Yokozawa T. Protective effect of persimmon (Diospyros kaki) peel proanthocyanidin against oxidative damage under $H_2O_2$-induced cellular senescence. Biol. Pharm. Bull. 31: 1265-1269 (2008) https://doi.org/10.1248/bpb.31.1265
  22. Lee YA, Cho EJ, Yokozawa T. Effects of proanthocyanidin preparations on hyperlipidemia and other biomarkers in mouse model of type 2 diabetes. J. Agr. Food Chem. 56: 7781-7789 (2008) https://doi.org/10.1021/jf800639m
  23. Omata N, Tsukahara H, Ito S, Ohshima Y, Yasutomi M, Yamada A, Jiang M, Hiraoka M, Nambu M, Deguchi Y, Mayumi M. Increased oxidative stress in childhood atopic dermatitis. Life Sci. 69: 223-228 (2001) https://doi.org/10.1016/S0024-3205(01)01124-9
  24. Gonzalez-Rey E, Chorny A, Delgado M. Regulation of immune tolerance by anti-inflammatory neuropeptides. Nat. Rev. Immunol. 7: 52-63 (2007) https://doi.org/10.1038/nri1984
  25. Kim MJ, Choung SY. Mixture of polyphenols and anthocyanins from Vaccinium uliginosum L. alleviates DNCB-induced atopic dermatitis in NC/Nga mice. Evid.-Based Compl. Alt. 2012: 1-15 (2012)
  26. Powrie F, Coffman RL. Cytokine regulation of T-cell function: Potential for therapeutic intervention. Trends Pharmacol. Sci. 14: 164-168 (1993) https://doi.org/10.1016/0165-6147(93)90202-U
  27. Leung DYM, Soter NA. Cellular and immunologic mechanisms in atopic dermatitis. J. Am. Acad. Dermatol. 44: S1-S12 (2001) https://doi.org/10.1067/mjd.2001.109815
  28. Sampson HA. Atopic dermatitis: immunological mechanisms in relation to phenotype. Pediatr. Allergy Immu. 12: 62-68 (2001) https://doi.org/10.1034/j.1399-3038.2001.121415.x
  29. Spergel JM, Mizoguchi E, Oettgen H, Bhan AK, Geha RS. Roles of $T_H1$ and $T_H2$ cytokines in a murine model of allergic dermatitis. J. Clin. Invest. 103: 1103-1111 (1999) https://doi.org/10.1172/JCI5669
  30. Jin CH, Lee HJ, Park YD, Choi DS, Kim DS, Kang SY, Seo KI, Jeong IY. Isoegomaketone inhibits lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages through the heme oxygenase-1 induction and inhibition of the interferon-${\beta}$-STAT-1 pathway. J. Agr. Food Chem. 58: 860-867 (2010) https://doi.org/10.1021/jf9033333
  31. Kalinski P. Regulation of immune responses by prostaglandin $E_2$. J. Immunol. 188: 21-28 (2012) https://doi.org/10.4049/jimmunol.1101029
  32. Huang N, Hauck C, Yum MY, Rizshsky L, Widrlechner MP, McCoy JA, Murphy PA, Dixon PM, Nikolau BJ, Birt DF. Rosmarinic acid in Prunella vulgaris ethanol extract inhibits lipopolysaccharide-induced prostaglandin E2 and nitric oxide in RAW 264.7 mouse macrophages. J. Agr. Food Chem. 57: 10579-10589 (2009) https://doi.org/10.1021/jf9023728
  33. De Nardin E. The role of inflammatory and immunological mediators in periodontitis and cardiovascular disease. Ann. Periodontol. 6: 30-40 (2001) https://doi.org/10.1902/annals.2001.6.1.30
  34. Seo MJ, Kang BW, Kim MJ, Lee HH, Seo KI, Kim KH, Jeong YK. The effect of cordycepin on the production of pro-inflammatory cytokines in mouse peritoneal macrophages. Korean J. Food Sci. Technol. 46: 68-72 (2014) https://doi.org/10.9721/KJFST.2014.46.1.68
  35. Cho YK, Kim HS, Kim JW, Lee SY, Kim WS, Ryu JH, Lim GB. Extraction of glabridin from licorice using supercritical carbon dioxide. Korean J. Biotechnol. Bioeng. 19: 427-432 (2004)
  36. Kim HS, Lee SY, Ryu JH, Lim GB, Kim BY, Kim WS, Lee EK. Extraction of glycyrrhizic acid from licorice using supercritical carbon dioxide/aqueous ethanol. Korea Soc. Biotechnol. Bioeng. J. 18: 347-351 (2003)
  37. Lee EJ, Yang SA, Choi HD, Im HG, Whang K, Lee IS. Comparison of gingerols in various fractions and the antioxidant effects of supercritical fluid extracts from ginger. Korean J. Food Sci. Technol. 43: 469-474 (2011) https://doi.org/10.9721/KJFST.2011.43.4.469
  38. Zhang Y, Wang X, Ma L, Dong L, Zhang X, Chen J, Fu X. Anti-inflammatory, antinociceptive activity of an essential oil recipe consisting of the supercritical fluid $CO_2$ extract of white pepper, long pepper, cinnamon, saffron and myrrh in vivo. J. Oleo Sci. 63: 1251-1260 (2014) https://doi.org/10.5650/jos.ess14061

Cited by

  1. Effects of Phytoncide-containing Cotton Fabrics on the Development of Atopic Dermatitis vol.52, pp.6, 2015, https://doi.org/10.12772/TSE.2015.52.388