Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
권호 표지
DOI QR코드

DOI QR Code

A Study for Preparation of Nano Ethosome Loaded with Resveratrol

레스베라트롤을 함유한 나노 에토좀 제조에 관한 연구

  • 서동환 (코스맥스 R&I center) ;
  • 윤종혁 (코스맥스 R&I center) ;
  • 김연준 (코스맥스 R&I center) ;
  • 변상요 (아주대학교 대학원 응용생명공학과 화장품과학전공)
  • Received : 2015.06.04
  • Accepted : 2015.06.23
  • Published : 2015.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Resveratrol is a natural polyphenol. It protects skin from skin injury, ultraviolet radiation and pathogenic attack. This study is to find the optimum condition for the preparation of ethosome loading amount of resveratrol in ethosome. Ethosomes were prepared by modified hydrated liquid crystal method. Investigation of factors affecting the entrapment efficiency and particle size of ethosomes was carried out. The particle size of ethosome were measured by particle analyzer. The loading efficiency of resveratrol in ethosome was measured by HPLC. The particle sizes were 111.2 ~ 112.8 nm and the loading efficiency of resveratrol was 81.25 ~ 88.75%. The optimum conditions for the preparation of ethosome was obtained from of lecithin : resveratrol : cholesterol : ethanol at a weight ratio of 2.0 : 0.08 : 0.05 : 20.0.

레스베라트롤(Resveratrol)은 천연 폴리페놀이며 피부 손상, 자외선 및 병원성의 공격으로부터 피부를 보호한다. 본 연구에서는 레스베라트롤을 함유한 에토좀의 최적의 제조 조건을 찾기 위해 시도하였다. 에토좀은 수화액정법을 응용하여 제조하였다. 에토좀의 봉입효율 및 입자 크기에 영향을 미치는 요인에 대하여 조사하였다. 에토좀의 입자 사이즈는 입도분석기를 이용하였다. 봉입효율은 HPLC를 이용하여 측정하였다. 에토좀 입자의 크기는 111.2 ~ 112.8 nm이고, 봉입효율은 81.25 ~ 88.75%임을 확인하였다. 최적의 제조 조건으로는 레시틴 : 레스베라트롤 : 콜레스테롤 : 에탄올 = 2.0 : 0.08 : 0.05 : 20.0% (w/w)의 비율로 제조된 경우임을 확인하였다.

Keywords

References

  1. O. Braun-Falco, H. C. Korting, and H. I. Mailbach, Liposome Dermatics, Spinger-Verlag, 70 (1992).
  2. C. Kirby and G. Gregoriadis, Dehydration-Rehydration Vesicles: A Simple Method for High Yield Drug Entrapment in Liposomes, Nature Biotechnology, 2, 979 (1984). https://doi.org/10.1038/nbt1184-979
  3. R. R. C. New, Liposomes: A Practical Approach, Oxford University Press, Oxford (1990).
  4. G. Gregoriadis, Liposome Technology 2nd, CRC Press, Florida (1993).
  5. J. M. Lopez-Pinto, M. L. Gonzalez-Rodriguez, and A. M. Rabasco, Effect of cholesterol and ethanol on dermal delivery from DPPC liposomes, Int., J. Pharm, 298(1), 1 (2005). https://doi.org/10.1016/j.ijpharm.2005.02.021
  6. E. Touitou, N. Dayana, L. Bergelsonb, B. Godina, and M. Eliaza, Ethosomes-novel vesicular carriers for enhanced delivery: characterization and skin penetration properties, J. Control. Release, 65(3), 403 (2000). https://doi.org/10.1016/S0168-3659(99)00222-9
  7. Y. A. Kim, S. H. Rhee, W. H. Lee, K. Y. Park, and Y. H. Choi, Molecular Biological Mechanisms of Resveratrol for Cancer Chemoprevention, J. Cancer Prev., 9, 19 (2004).
  8. I. Gulcin, Antioxidant properties of resveratrol: A structure-activity insight, innov. food sci. emerg. technol, 11, 210 (2010). https://doi.org/10.1016/j.ifset.2009.07.002
  9. N. R. Jo, S. A. Park, S. H. Jeon, J. H. Ha, and S. N. Park, Cellular protective effects and antioxidative activity of resveratrol, Appl. Chem. Eng., 24(5), 483 (2013). https://doi.org/10.14478/ace.2013.24.5.483
  10. P. Bernard and J. Y. Berthon, Resveratrol: an original mechanism on tyrosinase inhibition, Int. J. Cosmet. Sci., 22(3), 219 (2000). https://doi.org/10.1046/j.1467-2494.2000.00019.x
  11. K. Subbaramaiah, W. J. Chung, P. Michaluart, N. Telang, T. Tanabe, H. Inoue, M. Jang, J. M. Pezzuto, and A. J. Dannenberg, Resveratrol inhibits cyclooxygenase-2 transcription and activity in phorbol ester-treated human mammary epithelial cells, J. Biol. Chem., 273, 21875 (1998). https://doi.org/10.1074/jbc.273.34.21875
  12. B. S. Jin, S. M. Lee, and K. H. Lee, A study on the factors affecting entrapment efficiency and particle size of ethosomes, J. Korean. Ind. Eng. Chem., 17(2), 138 (2006).
  13. H. Y. Kwon, J. Y. Lee, S. W. Choi, Y. S. Jang, and J. H. Kim, Preparation of PLGA nanoparticles containing estrogen by emulsification-diffusion method, Colloids Surf., A, 182(1-3), 123 (2001). https://doi.org/10.1016/S0927-7757(00)00825-6
  14. P. Kan, Z. B. Chen, R. Y. Kung, C. J. Lee, and I. M. Chu, Study on the formulation of o/w emulsion as carriers for lipophilic drugs, Colloids Surf. B Biointerfaces, 15(2), 117 (1999). https://doi.org/10.1016/S0927-7765(99)00048-X
  15. M. Jumaa and B. W Muller, Parenteral emulsions stabilized with a mixture of phospholipids and PEG-660-12-hydroxy-stearate: evaluation of accelerated and long-term stability, Eur. J. Pharm. Biopharm., 54(2), 207 (2002). https://doi.org/10.1016/S0939-6411(02)00057-7
  16. B. S. Jin, Y. M. Han, K. H. Lee, and M. J. Choi, Preparation and characterization of Calcein entrapped ethosome, J. Korean Ind. Eng. Chem., 15(2), 205 (2004).
  17. J. J. Collins and M. C. Phillips The stability and structure of cholesterol-rich codispersions of cholesterol and phosphatidylcholine, J. Lipid Res., 23, 291 (1982).
  18. L. P. Tseng, H. J. Liang, T. W. Chung, Y. Y. Huang, and D. Z. Liu, Liposomes incorporated with cholesterol for drug release triggered by magnetic field, J. Med. Biol. Eng., 27(1), 29 (2007).
  19. H. J. Gwak and B. S, Jin, Preparation and characterization of EGCG entrapped ethosome, J. Korean Ind. Eng. Chem., 18(2), 130 (2007).