Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Effects of Tho2, a component of THO complex, on growth and mRNA export in fission yeast

분열효모에서 THO 복합체의 구성요소인 Tho2가 생장 및 mRNA export에 미치는 영향

  • Koh, Eun-Jin (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University) ;
  • Yoon, Jin Ho (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University)
  • 고은진 (성신여자대학교 생명과학.화학부 및 기초과학연구소) ;
  • 윤진호 (성신여자대학교 생명과학.화학부 및 기초과학연구소)
  • Received : 2015.05.26
  • Accepted : 2015.06.23
  • Published : 2015.06.30
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Abstract

Tho2/THOC2 is a subunit of the THO complex that plays important roles in mRNP biogenesis connecting transcription with mRNA maturation and export. A fission yeast, Schizosaccharomyces pombe, ortholog of Tho2/THOC2 was identified from the genome database. Tetrad analysis showed that the S. pombe tho2 is essential for growth. Repression or overexpression of the tho2 gene caused growth defect with elongated cells, abnormal DNA distribution, and accumulation of $poly(A)^+$ RNA in the nucleus. And the functional GFP-Tho2 protein is localized mainly in the nucleus. Yeast two-hybrid analysis showed that Tho2 interacted with Tex1, another subunit of THO complex. These results suggest that S. pombe Tho2 is also involved in mRNA export from the nucleus and is a component of THO complex.

Tho2/THOC2는 전사 과정을 mRNA 성숙 및 방출과 연결함으로써 mRNP의 생성에 중요한 역할을 담당하는 THO 복합체의 구성인자이다. 분열효모 Schizosaccharomyces pombe의 유전체 데이터에서 Tho2/THOC2의 이종상동체를 찾아 기능을 분석하였다. 4분체 분석 결과 이 유전자는 생장에 필수적이었다. S. pombe tho2 유전자의 발현을 억제하거나 과발현시키면 생장이 저해되는데, 세포의 길이가 길어지고 비정상적인 DNA분포와 $poly(A)^+$ RNA가 핵 안에 축적되는 표현형을 보였다. 또한 정상적인 기능을 가진 GFP-Tho2 단백질은 주로 핵 안에 존재하였다. Yeast two-hybrid 분석에서 Tho2는 THO 복합체의 또 다른 구성인자인 Tex1과 상호작용을 하였다. 이와 같은 결과들은 S. pombe의 Tho2 상동체도 THO 복합체의 구성인자로 mRNA 방출에 관여하고 있음을 시사한다.

Keywords

References

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