DOI QR코드

DOI QR Code

16S rRNA 기초 새송이 버섯(Pleurotus eryngii)의 식용가능 부위 내생세균 군집 다양성

Molecular diversity of endobacterial communities in edible part of King oyster mushroom (Pleurotus eryngii) based on 16S rRNA

  • Lee, Choung Kyu (Department of Forest Resources, Gyeongnam National University of Science and Technology) ;
  • Haque, Md. Azizul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Choi, Byoung Rock (Department of Future Farming, Sacheon Agricultural Development & Technology Center) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Hwang, Chung Eun (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Ahn, Min Ju (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology)
  • 투고 : 2014.12.17
  • 심사 : 2015.06.10
  • 발행 : 2015.06.30

초록

세균 16S rRNA 유전자 특이 프라이머를 기초로 하여 중합 효소연쇄반응을 이용하여 Pleurotus eryngii (큰느타리 버섯)의 내부에 존재하는 세균의 다양성을 조사하였다. 세균 16S rRNA의 라이브러리는 버섯 갓(BC, body cap)와 줄기(BS, body stipe)로 구성하였다. BC 20 클론은 네 그룹으로 구별되었고 가장 큰 그룹은 Firmicutes (클론의 40%) 있었다. 그러나, BS 20 클론은 여섯 그룹으로 나뉘어졌고 가장 큰 그룹은 Actinobacteria (클론의 40%) 있었다. 전체 버섯 내에 존재하는 세균 그룹은 그람양성 세균(62.5%) 있었다.

The diversity of endobacteria in the edible part (cap and stipe) king oyster mushroom (Pleurotus eryngii) was investigated using 16S rRNA sequence analysis. The bacterial 16S rRNA libraries were constructed from the body cap (BC) and the body stipe (BS) of the king oyster mushroom. The twenty sequenced BC clones were divided into four groups and the largest group was affiliated with the Firmicutes (40% of clones). While, the twenty sequenced BS clones could be divided into six groups and the largest group was affiliated with the Actinobacteria (40% of clones). The predominant bacterial family from both the cap and stipe of the mushroom was corresponded with the Gram positive bacteria (62.5%).

키워드

참고문헌

  1. Barbieri, E., Bertini, L., Rossi, I., Ceccaroli, P., Saltarelli, R., Guidi, C., Zambonelli, A., and Stocchi, V. 2005. New evidence for bacterial diversity in the ascoma of the ectomycorrhizal fungus Tuber borchii Vittad. FEMS Microbiol. Lett. 247, 23-35. https://doi.org/10.1016/j.femsle.2005.04.027
  2. Barbieri, E., Potenza, L., Rossi, I., Sisti, D., Giomaro, G., Rossetti, S., Beinfohr, C., and Stocchi, V. 2000. Phylogenetic characterisation and in situ detection of a Cytophaga-Flexibacter-Bacteroides phylogroup bacterium in Tuber borchii Vittad. ectomycorrhizal mycelium. Appl. Environ. Microbiol. 66, 5035-5042. https://doi.org/10.1128/AEM.66.11.5035-5042.2000
  3. Barbieri, E., Riccioni, G., Pisano, A., Sisti, D., Zeppa, S., Agostini, D., and Stocchi, V. 2002. Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium. Appl. Environ. Microbiol. 68, 6421-6424. https://doi.org/10.1128/AEM.68.12.6421-6424.2002
  4. Bedini, S., Bagnoli, G., Sbrana, C., Leporini, C., Tola, E., Dunne, C., Filippi, C., D'Andrea, F., O'Gara, F., and Nuti, M.P. 1999. Pseudomonas isolated from within fruit bodies of Tuber borchi are capable of producing biological control or photostimulatory compounds in pure culture. Symbiosis 26, 223-236.
  5. Bertaux, J., Schmid, M., Prevost-Boure, N.C., Churin, J.L., Hartmann, A., Garbaye, J., and Frey-Klett, P. 2003. In situ identification of intracellular bacteria related to Paenibacillus spp. in the mycelium of the ectomycorrhizal fungus Laccaria bicolor S238N. Appl. Environ. Microbiol. 69, 4243-4248. https://doi.org/10.1128/AEM.69.7.4243-4248.2003
  6. Bertaux, J., Schmid, M., Hutzler, P., Hartmann, A., Garbaye, J., and Frey-Klett, P. 2005. Occurrence and distribution of endobacteria in the plant-associated mycelium of the ectomycorrhizal fungus Laccaria bicolor S238N. Environ. Microbiol. 7, 1786-1795. https://doi.org/10.1111/j.1462-2920.2005.00867.x
  7. Bianciotto, V., Bandi, C., Minerdi, D., Sironi, M., Tichy, H.V., and Bonfante, P. 1996. An obligately endosymbiotic mycorrhizal fungus itself harbors obligately intracellular bacteria. Appl. Environ. Microbiol. 62, 3005-3010
  8. Fell, J.W., Boekhout, T., Fonseca, A., Scorzetti, G., and Statzell-Tallman, A. 2000. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50, 1351-1371. https://doi.org/10.1099/00207713-50-3-1351
  9. Frey-Klett, P., Chavatte, M., Clausse, M.L., Courrier, S., Le Roux, C., Raaijmakers, J., Martinotti, M.G., Pierrat, J.C., and Garbaye, J. 2005. Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads. New Phytol. 165, 317-328.
  10. Frey, P., Frey-Klett, P., Garbaye, J., Berge, O., and Heulin, T. 1997. Metabolic and genotypic fingerprinting of fluorescent pseudomonads associated with the douglas fir Laccaria bicolor mycorrhizosphere. Appl. Environ. Microbiol. 63, 1852-1860.
  11. Gao, X.X., Zhou, H., Xu, D.Y., Yu, C.H., Chen, Y.Q., and Qu, L.H. 2005. High diversity of endophytic fungi from the pharmaceutical plant, Heterosmilax japonica Kunth revealed by cultivationindependent approach. FEMS Microbiol. Lett. 249, 255-266. https://doi.org/10.1016/j.femsle.2005.06.017
  12. Gazzanelli, G., Malatesta, M., Pianetti, A., Baffone, W., Stocchi, V., and Citterio, B. 1999. Bacteria associated to fruit bodies of the ecto-mycorrhizal fungus Tuber borchii Vittad. Symbiosis 26, 211-219.
  13. Hall, I.R., Yun, W., and Amicucci, A. 2003. Cultivation of edible ectomycorrhizal mushrooms. Trends Biotechnol. 21, 433-438. https://doi.org/10.1016/S0167-7799(03)00204-X
  14. Hashiba, T. and Narisawa, K. 2005. The development and endophytic nature of the fungus Heteroconium chaetospira. FEMS Microbiol. Lett. 252, 191-196. https://doi.org/10.1016/j.femsle.2005.08.039
  15. Kang, Y.M. and Cho, K.M. 2014. Identification of auxin from Pseudomonas sp. P7014 for the rapid growth of Pleurotus eryngii mycelium. Korean J. Microbiol. 50, 15-21. https://doi.org/10.7845/kjm.2014.3076
  16. Lane, D.J. 1991. 16S/23S rRNA sequencing. Nucleic acids techniques in bacterial systematics, p. 115. In Stackebrandt, E. and Goodfellow, M. (eds.) John Wiley and Sons, Chicester, UK.
  17. Madden, T.L., Tatusov, R.L., and Zhang, J. 1996. Applications of network BLAST server. Methods Enzymol. 266, 131-141. https://doi.org/10.1016/S0076-6879(96)66011-X
  18. Maidak, B.L., Cole, J.R., Lilburn, T.G., Parker Jr., C.T., Saxman, P.R., Stredwick, J.M., Garrity, G.M., Li, B., Olsen, G.J., Pramanik, S., et al. 2000. The RDP (Ribosomal Database Project) continues. Nucleic Acids Res. 28, 173-174. https://doi.org/10.1093/nar/28.1.173
  19. Moran, N.A. and Wernegreen, J.J. 2000. Life style evolution in symbiotic bacteria: insights from genomics. Trends Ecol. Evol. 15, 321-326. https://doi.org/10.1016/S0169-5347(00)01902-9
  20. Morioka, M. and Ishikawa, H. 1993. Self-assembly of symbionin, a chaperonin of intracellular symbiont. J. Biochem. 114, 468-472. https://doi.org/10.1093/oxfordjournals.jbchem.a124201
  21. Mota, L.J., Sorg, I., and Cornelis, G.R. 2005. Type III secretion: The bacteria-eukaryotic cell express. FEMS Microbiol. Lett. 252, 1-10. https://doi.org/10.1016/j.femsle.2005.08.036
  22. Paster, B.J., Dewhirst, F.E., Weisburg, W.G., Tordoff, L.A., Fraser, G.J., Hespell, R.B., Stanton, T.B., Zablen, L., Mandelco, L., and Woese, C.R. 1991. Phylogenetic analysis of the spirochetes. J. Bacteriol. 173, 6101-6109. https://doi.org/10.1128/jb.173.19.6101-6109.1991
  23. Perotti, R. 1926. On the limits of biological enquiry in soil science. Proc. Int. Soc. Soil Sci. 2, 146-161
  24. Philippoussis, A., Zervakis, G., and Diamantopoulou, P. 2001. Bioconversion of agricultural lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World J. Microbiol. Biotechnol. 17, 191-200. https://doi.org/10.1023/A:1016685530312
  25. Ruiz-Duenas, F.J. and Martinez, M.J. 1996. Enzymatic activities of Trametes versicolor and Pleurotus eryngii implicated in biocontrol of Fusarium oxysporum f. sp. lycopersici. Curr. Microbiol. 32, 151-155. https://doi.org/10.1007/s002849900027
  26. Saitou, N. and Nei, M. 1997. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
  27. Sbrana, C., Agnolucci, M., Bedini, S., Lepera, A., Toffanin, A., Giovannetti, M., and Nuti, M.P. 2002. Diversity of culturable bacterial populations associated to Tuber borchii ectomycorrhizas and their activity on T. borchii mycelial growth. FEMS Microbiol. Lett. 211, 195-201. https://doi.org/10.1111/j.1574-6968.2002.tb11224.x
  28. Sbrana, C., Bagnoli, G., Bedini, S., Filippi, C., Giovannetti, M., and Nuti, M.P. 2000. Adhesion to hyphal matrix and antifungal activity of Pseudomonas strains isolated from Tuber borchii ascocarps. Can. J. Microbiol. 46, 259-268. https://doi.org/10.1139/cjm-46-3-259
  29. Schussler, A. and Kluge, M. 2000. Geosiphon pyriforme, an endocytosymbiosis between fungus and cyanobacteria, and its meaning as a model system for AM research, p. 151. The mycota IX (ed.), In Hock, B. (ed.). Springer Verlag, Berlin, Germany
  30. Schußler, A., Schwarzott, D., and Walker, C. 2001. A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycol. Res. 105, 1413-1421. https://doi.org/10.1017/S0953756201005196
  31. Schulz, B., Boyle, C., Draeger, S., Rommert, A.K., and Krohn, K. 2002. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol. Res. 106, 996-1004. https://doi.org/10.1017/S0953756202006342
  32. Steyn, P.L., Segers, P., Vancanneyt, M., Sandra, P., Kersters, K., and Joubert, J.J. 1998. Classification of heparinolytic bacteria into a new genus, Pedobacter, comprising four species: Pedobacter heparinus comb. nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. proposal of the family Sphingobacteriaceae fam. nov. Int. J. Syst. Bacteriol. 48, 165-177. https://doi.org/10.1099/00207713-48-1-165
  33. Timonen, S., Jorgensen, K.S., Haahtela, K., and Sen, R. 1998. Bacterial community structure at defined locations of Pinus sylvestris-Suillus bovinus and Pinus sylvestris-Paxillus involutus mycorrhizospheres in dry pine forest humus and nursery peat. Can. J. Microbiol. 44, 499-513. https://doi.org/10.1139/cjm-44-6-499
  34. Tompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  35. Wang, H. and Ng, T.B. 2004. Eryngin, a novel antifungal peptide from fruiting bodies of the edible mushroom Pleurotus eryngiij Peptides 25, 1-5. https://doi.org/10.1016/j.peptides.2003.11.014
  36. Wasser, S.P. and Weis, A.L. 1999. Medicinal properties of substances occurring in higher Basidiomycetes mushrooms: current perspectives. Int. J. Med. Mushr. 1, 47-50.

피인용 문헌

  1. Growing edible mushrooms: a conversation between bacteria and fungi vol.22, pp.3, 2015, https://doi.org/10.1111/1462-2920.14765