Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Soybean Peptides Induce Apoptosis in HeLa Cells by Increasing Oxidative Stress

  • Sung, Ho Joong (Department of Biomedical Laboratory Science, College of Health Science, Eulji University) ;
  • Jeong, Yu Jin (Department of Biomedical Laboratory Science, College of Health Science, Eulji University) ;
  • Kim, Jihyun (Department of Biomedical Laboratory Science, College of Health Science, Eulji University) ;
  • Jung, Eunsun (Biospectrum Life Science Institute) ;
  • Jun, Jin Hyun (Department of Biomedical Laboratory Science, College of Health Science, Eulji University)
  • Received : 2015.05.14
  • Accepted : 2015.06.22
  • Published : 2015.06.30
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Abstract

Soy proteins have been extensively studied because of its multiple health benefits. However, the effects of soy proteins on human cervical cancer cells are still unclear. Therefore, this study investigated the effects of soy proteins on HeLa cells and human fibroblasts by using soybean peptides (SPs). SPs selectively increased the generation of reactive oxygen species and apoptosis in HeLa cells but not in fibroblasts. In addition, SPs suppressed the migration of HeLa cells. Although the molecular mechanisms underlying the effects of SPs on human cervical cancer cells need to be investigated further, our findings provide insights on the therapeutic effects of soy protein on cervical cancer.

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References

  1. Appelt LC, Reicks MM. Soy induces phase II enzymes but does not inhibit dimethylbenz[a]anthracene-induced carcinogenesis in female rats. J Nutr. 1999. 129: 1820-1826. https://doi.org/10.1093/jn/129.10.1820
  2. Clark RA, Lanigan JM, DellaPelle P, Manseau E, Dvorak HF, Colvin RB. Fibronectin and fibrin provide a provisional matrix for epidermal cell migration during wound reepithelialization. J Invest Dermatol. 1982. 79: 264-269. https://doi.org/10.1111/1523-1747.ep12500075
  3. Coussens LM, Werb Z. Inflammation and cancer. Nature. 2002. 420: 860-867. https://doi.org/10.1038/nature01322
  4. Djuric Z, Chen G, Doerge DR, Heilbrun LK, Kucuk O. Effect of soy isoflavone supplementation on markers of oxidative stress in men and women. Cancer Lett. 2001. 172: 1-6. https://doi.org/10.1016/S0304-3835(01)00627-9
  5. Food and Drug Administration, HHS, Final rule. Food labeling: health claims; soy protein and coronary heart disease. Fed Regist. 1999. 64: 57700-57733.
  6. Gold R, Schmied M, Giegerich G, Breitschopf H, Hartung HP, Toyka KV, Lassmann H. Differentiation beteween cellular apoptosis and necrosis by the combined use of in situ tailing and nick translational techniques. Lab Invest. 1994. 71: 219-225.
  7. Istfan N, Murray E, Janghorbani M, Evans WJ, Young VR. The nutritional value of a soy protein concentrate (STAPRO-3200) for long-term protein nutritional maintenance in young men. J Nutr. 1983. 113: 2524-2534. https://doi.org/10.1093/jn/113.12.2524
  8. Jacobsen BK, Knutsen SF, Fraser GE. Does high soy milk intake reduce prostate cancer incidence? The Adventist Health Study (United States). Cancer Causes Control. 1998. 9: 553-557. https://doi.org/10.1023/A:1008819500080
  9. Kim SY, Kim SJ, Lee JY, Kim WG, Park WS, Sim YC, Lee SJ. Protective effects of dietary soy isoflavones against UV-induced skin-aging in hairless mouse model. J Am Coll Nutr. 2004. 23: 157-162. https://doi.org/10.1080/07315724.2004.10719356
  10. Kim YS, Sull JW, Sung HJ. Suppressing effect of resveratrol on the migration and invasion of human metastatic lung and cervical cancer cells. Mol Biol Rep. 2012. 39: 8709-8716. https://doi.org/10.1007/s11033-012-1728-3
  11. Kirk EA, Sutherland P, Wang SA, Chait A, LeBoeuf RC. Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice. J Nutr. 1998. 128: 954-959. https://doi.org/10.1093/jn/128.6.954
  12. Kumar B, Koul S, Khandrika L, Meacham RB, Koul HK. Oxidative stress is inherent in prostate cancer cells and is required for aggressive phenotype. Cancer Res. 2008. 68: 1777-1785. https://doi.org/10.1158/0008-5472.CAN-07-5259
  13. Lee J, Roh KB, Kim SC, Park D. Soy peptide-induced stem cell proliferation: involvement of ERK and TGF-beta1. J Nutr Biochem. 2012. 23: 1341-1351. https://doi.org/10.1016/j.jnutbio.2011.08.003
  14. Ma TC, Campana A, Lange PS, Lee HH, Banerjee K, Bryson JB, Mahishi L, Alam S, Giger RJ, Barnes S, Morris SM, Jr., Willis DE, Twiss JL, Filbin MT, Ratan RR. A large-scale chemical screen for regulators of the arginase 1 promoter identifies the soy isoflavone daidzeinas a clinically approved small molecule that can promote neuronal protection or regeneration via a cAMP-independent pathway. J Neurosci. 2010. 30: 739-748. https://doi.org/10.1523/JNEUROSCI.5266-09.2010
  15. Mahn K, Borras C, Knock GA, Taylor P, Khan IY, Sugden D, Poston L, Ward JP, Sharpe RM, Vina J, Aaronson PI, Mann GE. Dietary soy isoflavone induced increases in antioxidant and eNOS gene expression lead to improved endothelial function and reduced blood pressure in vivo. FASEB J. 2005. 19: 1755-1757. https://doi.org/10.1096/fj.05-4008fje
  16. Martin P. Wound healing--aiming for perfect skin regeneration. Science. 1997. 276: 75-81. https://doi.org/10.1126/science.276.5309.75
  17. Nizamutdinova IT, Kim YM, Chung JI, Shin SC, Jeong YK, Seo HG, Lee JH, Chang KC, Kim HJ. Anthocyanins from black soybean seed coats stimulate wound healing in fibroblasts and keratinocytes and prevent inflammation in endothelial cells. Food Chem Toxicol. 2009. 47: 2806-2812. https://doi.org/10.1016/j.fct.2009.08.016
  18. Omoni AO, Aluko RE. Soybean foods and their benefits: potential mechanisms of action. Nutr Rev. 2005. 63: 272-283. https://doi.org/10.1111/j.1753-4887.2005.tb00141.x
  19. Pelicano H, Carney D, Huang P. ROS stress in cancer cells and therapeutic implications. Drug Resist Updat. 2004. 7: 97-110. https://doi.org/10.1016/j.drup.2004.01.004
  20. Saleem M. Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett. 2009. 285: 109-115. https://doi.org/10.1016/j.canlet.2009.04.033
  21. Schroder K, Helmcke I, Palfi K, Krause KH, Busse R, Brandes RP. Nox1 mediates basic fibroblast growth factor-induced migration of vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 2007. 27: 1736-1743. https://doi.org/10.1161/ATVBAHA.107.142117
  22. Sung HJ, Ma W, Wang PY, Hynes J, O'Riordan TC, Combs CA, McCoy JP, Jr., Bunz F, Kang JG, Hwang PM. Mitochondrial respiration protects against oxygen-associated DNA damage. Nat Commun. 2010. 1: 5.
  23. Tham DM, Gardner CD, Haskell WL. Clinical review 97: Potential health benefits of dietary phytoestrogens: a review of the clinical, epidemiological, and mechanistic evidence. J Clin Endocrinol Metab. 1998. 83: 2223-2235.
  24. Ullah MF, Ahmad A, Zubair H, Khan HY, Wang Z, Sarkar FH, Hadi SM. Soy isoflavone genistein induces cell death in breast cancer cells through mobilization of endogenous copper ions and generation of reactive oxygen species. Mol Nutr Food Res. 2011. 55: 553-559. https://doi.org/10.1002/mnfr.201000329
  25. Wiseman H, O'Reilly JD, Adlercreutz H, Mallet AI, Bowey EA, Rowland IR, Sanders TA. Isoflavone phytoestrogens consumed in soy decrease F(2)-isoprostane concentrations and increase resistance of low-density lipoprotein to oxidation in humans. Am J Clin Nutr. 2000. 72: 395-400. https://doi.org/10.1093/ajcn/72.2.395
  26. Xiao J, Huang G, Geng X, Qiu H. Soy-derived isoflavones inhibit hela cell growth by inducing apoptosis. Plant Foods Hum Nutr. 2011. 66: 122-128. https://doi.org/10.1007/s11130-011-0224-6
  27. Yoshikoshi M, Yoshiki Y, Okubo K, Seto J, Sasaki Y. Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblasts. Planta Med. 1996. 62: 252-255. https://doi.org/10.1055/s-2006-957871
  28. Zhang R, Humphreys I, Sahu RP, Shi Y, Srivastava SK. In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis. 2008. 13: 1465-1478. https://doi.org/10.1007/s10495-008-0278-6
  29. Zhuo XG, Melby MK, Watanabe S. Soy isoflavone intake lowers serum LDL cholesterol: a meta-analysis of 8 randomized controlled trials in humans. J Nutr. 2004. 134: 2395-2400. https://doi.org/10.1093/jn/134.9.2395