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Genotype Analyses of Methicillin Resistant Staphylococcus aureus Isolated from clinical specimens

임상검체로부터 분리된 Methicillin 내성 Staphylococcus aureus의 유전자형 분석

  • Kim, Jean-Soo (Dept. of Clinical Pathology, Health Institute of Technology) ;
  • Park, Chul (Dept. of Clinical Pathology, Gwangyang Health College)
  • 김진수 (대전보건대학교 임상병리과) ;
  • 박철 (광양보건대학교 임상병리과)
  • Received : 2015.02.13
  • Accepted : 2015.05.07
  • Published : 2015.05.31
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Abstract

Staphylococcus aureus is the major causative organism of nasocomial infection being the important pathogen in the clinic. Appearance of staphylococcus aureus resistant to methicillin (MRSA) is becoming a big problem in clinics and dynamics all over the world acquiring antibiotic resistance with virulence factors as its feature differentiated from other pathogenic bacteria fast. This research intended to compare and analyze the correlation of antibiotics resistance between strains with toxin genes and distribution of toxin genes of MRSA 101 strains acquired from clinical specimen in one general hospital (enterotoxin(se), toxic shock syndrome toxin-1(tst), exfoliative toxin(et), Panton Valentine leukocidin(pvl)). seg gene, isolated the most among toxin genes, was detected in 59 strains (58.4%) and more than two toxin genes were detected in 70 strains (69.3%). As a combination possessing toxin genes, it was detected in 19 strains (18.8%) as seb, sec, seg, sei, tst and the second frequent combination was sec, seg, sei shown in 11 strains (10.9%). 19 strains (18.8%) with combinations of toxin genes same with seb, sec, seg, sei, tst had 100% resistance Ampicillin, Benzylpenicillin, Ciprofloxacin, Clindamycin, Gentamicin, Erythromycin, Telithromycin, Tetracycline antibiotics. Strains with many toxin genes showed high correlation of antibiotic resistance. Afterwards, effective therapy and thorough infection management should be preceded not to spread the resistance of MRSA strain.

황색포도상구균은 임상에서 중요한 병원체로 원내감염의 주 원인균이다. methicillin에 내성인 황색포도상구균(MRSA) 출현은 특히 전 세계적으로 임상과 역학의 주요 문제가 되고 있으며 다른 병원성 세균과 차별되는 특징으로 독성인자를 가지면서 항생제 내성을 빠르게 획득해 가고 있다. 본 연구는 한 종합병원 임상 검체로부터 얻어진 MRSA 101균주의 독소유전자(enterotoxin(se), toxic shock syndrome toxin-1(tst), exfoliative toxin(et), Panton Valentine leukocidin(pvl)) 보유 분포와 이들 독소 유전자 보유 균주들간의 항생제 내성과의 상관성을 비교 분석 하고자 하였다. 독소유전자중 가장 많이 분리된 seg 유전자가 59균주(58.4%)에서 검출되었으며 70균주(69.3%)에서 두개 이상의 독소 유전자가 검출되었다. 독소유전자 보유조합으로 seb, sec, seg, sei, tst가 19균주(18.8%)에서 발견되었으며 다음으로 빈번한 조합은 sec, seg, sei로 11균주(10.9%)로 나타났다. seb, sec, seg, sei, tst 동일한 독소유전자 조합을 갖고 있는 19균주(18.8%)들은 Ampicillin, Benzylpenicillin, Ciprofloxacin, Clindamycin, Gentamicin, Erythromycin, Telithromycin, Tetracycline 항생제 내성이 100% 였다. 독소유전자 보유수가 많은 균주들은 항생제 내성이 높은 상관성을 확인하였다. 향후 MRSA 균주의 내성이 확산되지 않도록 효과적인 치료와 철저한 감염관리가 선행되어야 할 것이다.

Keywords

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