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http://dx.doi.org/10.5762/KAIS.2015.16.5.3315

Genotype Analyses of Methicillin Resistant Staphylococcus aureus Isolated from clinical specimens  

Kim, Jean-Soo (Dept. of Clinical Pathology, Health Institute of Technology)
Park, Chul (Dept. of Clinical Pathology, Gwangyang Health College)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.16, no.5, 2015 , pp. 3315-3322 More about this Journal
Abstract
Staphylococcus aureus is the major causative organism of nasocomial infection being the important pathogen in the clinic. Appearance of staphylococcus aureus resistant to methicillin (MRSA) is becoming a big problem in clinics and dynamics all over the world acquiring antibiotic resistance with virulence factors as its feature differentiated from other pathogenic bacteria fast. This research intended to compare and analyze the correlation of antibiotics resistance between strains with toxin genes and distribution of toxin genes of MRSA 101 strains acquired from clinical specimen in one general hospital (enterotoxin(se), toxic shock syndrome toxin-1(tst), exfoliative toxin(et), Panton Valentine leukocidin(pvl)). seg gene, isolated the most among toxin genes, was detected in 59 strains (58.4%) and more than two toxin genes were detected in 70 strains (69.3%). As a combination possessing toxin genes, it was detected in 19 strains (18.8%) as seb, sec, seg, sei, tst and the second frequent combination was sec, seg, sei shown in 11 strains (10.9%). 19 strains (18.8%) with combinations of toxin genes same with seb, sec, seg, sei, tst had 100% resistance Ampicillin, Benzylpenicillin, Ciprofloxacin, Clindamycin, Gentamicin, Erythromycin, Telithromycin, Tetracycline antibiotics. Strains with many toxin genes showed high correlation of antibiotic resistance. Afterwards, effective therapy and thorough infection management should be preceded not to spread the resistance of MRSA strain.
Keywords
nosocomial infections; methicillin resistant Staphylococcus aureus(MRSA); toxin gene; antibiotic resistance;
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