Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Enhanced drought and oxidative stress tolerance in transgenic sweetpotato expressing a codA gene

CodA 고발현 형질전환 고구마의 산화 및 건조 스트레스 내성 증가

  • Park, Sung-Chul (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Myoung Duck (Institute of Genetic Engineering, Hankyong National University (HNU)) ;
  • Kim, Sun Ha (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Yun-Hee (Department of Biology Education, College of Education, Gyeongsang National University) ;
  • Jeong, Jae Cheol (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Lee, Haeng-Soon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 박성철 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김명덕 (국립한경대학교 유전공학연구소) ;
  • 김선하 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김윤희 (국립경상대학교 사범대학 생물교육과) ;
  • 정재철 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 이행순 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 곽상수 (한국생명공학연구원 식물시스템공학연구센터)
  • Received : 2015.03.09
  • Accepted : 2015.03.20
  • Published : 2015.03.31
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Abstract

Glycine betaine (GB) is one of the compatible solutes that accumulate in the chloroplasts of certain halotolerant plants under salt or cold stress. The codA gene for choline oxidase, the enzyme that converts choline into GB, has been cloned from a soil bacterium Arthrobacter globiformis. We generated transgenic sweetpotato plants [Ipomoea batatas (L.) Lam] expressing codA gene in chloroplasts under the control of the SWPA2 promoter (referred to as SC plants) and evaluated SC plants under oxidative and drought stresses. SC plants showed enhanced tolerance to methyl viologen (MV)-mediated oxidative stress and drought stress due to induced expression of codA. At $5{\mu}M$ of MV treatment, all SC plants showed enhanced tolerance to MV-mediated oxidative stress through maintaining low ion leakage and increased GB levels compared to wild type plants. When plants were subjected to drought conditions, SC plants showed enhanced tolerance to drought stress through maintaining high relative water contents and increased codA expression compared to wild type plants. These results suggest that the SC plants generated in this study will be useful for enhanced biomass production on global marginal lands.

식물은 여러 환경스트레스에 적응하기 위해 스트레스 내성 유전자의 발현 혹은 proline, trehalose, glycine betaine (GB) 등과 같이 삼투압을 조절하는 compatible solute를 생성하면서 진화해 왔다. GB는 고염, 저온 등 환경스트레스 조건에서 식물의 엽록체에서 축적되는 물질 중 하나이다. 토양 박테리아 Arthrobacter globiformis에서 분리한 choline oxidase (codA) 유전자는 choline을 GB로 전환하는 기능을 한다. 본 연구에서는 산화스트레스 유도성 SWPA2 프로모터의 발현조절 하에 codA 유전자를 엽록체에 과발현시킨 형질전환 고구마 식물체(SC식물체)를 제작하여 다양한 환경스트레스 조건에서의 특성을 분석하였다. SC 식물체는 methyl viologen (MV)에 의한 산화스트레스와 건조 처리 조건에서 내성 증가를 보였다. $5{\mu}M$ MV 처리시 형질전환 식물체는 GB의 함량이 증가하였고 낮은 수준의 이온 전도도를 보였다. 건조 스트레스 조건에서 형질전환 식물체는 codA 유전자의 발현이 증가하였으며, 대조구 보다 높은 상대수분함량을 유지하였다. 따라서 본 연구결과의 SC식물체는 고염, 건조토양 등 조건 불리지역에 재배하면 바이오매스를 증가시킬 수 있을 것으로 예상된다.

Keywords

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