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Control Activities of Fungicides Against Garlic White Rot Caused by Sclerotium cepivorum

마늘 흑색썩음균핵병에 대한 살균제의 작용 특성

  • Kim, Heongjo (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University) ;
  • Min, Yi Gi (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
  • 김형조 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과) ;
  • 민이기 (충북대학교 농업생명환경대학 식물의학과)
  • Received : 2015.02.06
  • Accepted : 2015.03.24
  • Published : 2015.03.31

Abstract

In order to control garlic white rot (Sclerotium cepivorum), which threatens garlic production in farmers fields, soil solarization (solar sterilization), sclerotia germination inducers and effective microorganisms as biological control agents, and chemical fungicides have been used. Among them, fungicide has been largely used to reduce garlic white rot. In this study, the antifungal activities of five fungicides, prochloraz(a.i. 25%, EC), tebuconazole (a.i. 25%, WP), flutolanil (a.i. 15%, EC), iminoctadine tris-albesilate (a.i. 40%, WP) and isoprothiolane (a.i. 40%, EC) with different mode of action, in mycelial growth, sclerotia germination and sclerotia production, were tested. The inhibitory effects of the 5 fungicides on the mycelial growth, and sclerotia germination and production of garlic white rot pathogen (S. cepivorum T11-2) were investigated on potato dextrose agar (PDA) and their control efficacies were evaluated on garlic flakes. There was no mycelial growth of S. cepivorum T11-2 on PDA amended with $0.8{\mu}g\;mL^{-1}$ of prochloraz or $100{\mu}g\;mL^{-1}$ of tebuconazole. Also prochloraz and tebuconazole inhibited perfectively the sclerotia germination of the pathogen at 10 and $1.0{\mu}g\;mL^{-1}$, respectively. In spite of a very low activity of isoprothiolane in mycelial growth and sclerotia germination of S. cepivorum T11-2, it showed a good inhibitory activity against sclerotia production of S. cepivorum T11-2 on PDA amended with $1.67{\mu}g\;mL^{-1}$. Prochloraz, tebuconazole and flutolanil showed above 70% of control value when they were treated at $100{\mu}g\;mL^{-1}$ using the garlic flake cutting-method.

마늘 흑색썩음균핵병(Sclerotium cepivorum)은 마늘 생산에 큰 영향을 미치는 주요한 토양병으로, 이를 방제하기 위하여 태양열 소독, 균핵발아 유도물질의 사용, 미생물을 이용한 생물적 방제, 살균제 처리 등 다양한 방법이 활용되고 있다. 그 중에서 살균제를 사용하는 방법을 가장 실용성이 높은 방법이다. 본 실험에서는 살균제의 작용기작이 다른 prochloraz (a.i. 25%, EC), tebuconazole (a.i. 25%, WP), flutolanil (a.i. 15%, EC), iminoctadine trisalbesilate (a.i. 40%, WP), isoprothiolane (a.i. 40%, EC) 5종의 살균제를 선정하여 병원균의 균사생장, 균핵발아, 균핵형성에 미치는 영향을 조사하였으며, 마늘 인편 절단법을 사용하여 공시한 살균제의 병 방제효과를 조사하였다. 병원균의 균사생장에 대해서는 prochloraz와 tebuconazole의 효과가 우수하였는데, prochloraz는 $0.8{\mu}g\;mL^{-1}$을, 그리고 tebuconazole은 $100{\mu}g\;mL^{-1}$을 첨가한 PDA 배지에서 병원균이 전혀 생장을 하지 못하였다. 또한 prochloraz는 $10{\mu}g\;mL^{-1}$의 처리구에서, 그리고 tebuconazole은 $1.0{\mu}g\;mL^{-1}$의 처리구에서 발아가 완전히 억제되었다. Isoprothiolane은 균사생장과 균핵발아에 대한 억제효과는 크기 못했지만, $1.67{\mu}g\;mL^{-1}$의 처리구에서 균핵의 형성을 63.5% 억제하였다. 마늘 인편 절단법을 사용하여 병 방제 효과를 조사한 결과, $100{\mu}g\;mL^{-1}$의 처리구에서 prochloraz, tebuconazole, flutolanil 등이 70% 이상의 효과를 보였다.

Keywords

References

  1. Andrea, T. B., Z. M. Emma, G. C. Carmen and F. C. Ronald (1996) The use of arbuscular mycorrhizae to control onion white rot (Sclerotium cepivorum Berk.) under field conditions. Mycorrhizae 6:253-257. https://doi.org/10.1007/s005720050133
  2. Cho W. D. and W. G. Kim (1996) Occurrence of white rot on Alliaceous vegetable crops. Korean J. Plant Pathol. 12:251-254.
  3. Coley-Smith, J. R., C. M. Mitchell and D. E. Sansford (1990) Long-term survival of Sclerotium cepivorum and Stromatinia gladioli. Plant Pathology 39:58-69. https://doi.org/10.1111/j.1365-3059.1990.tb02476.x
  4. Coley-Smith, J. R. and J. E. King (1969) Production of volatile alkyl sulphides by microbial degradation of synthetic alliin and alliin-like compounds, in relation to germination of sclerotia of Sclerotium cepivorum Berk. Ann. Appl. Biol. 64:303-314. https://doi.org/10.1111/j.1744-7348.1969.tb02880.x
  5. Crowe, F. J., D. H. Hall, A. S. Greathead and K. G. Baghott (1980) Inoculum density of Sclerotium cepivorum and the incidence of white rot of onion and garlic. Phytopathology 70:64-69. https://doi.org/10.1094/Phyto-70-64
  6. Earnshaw, D., M. R. McDonald and G. J. Boland (2000) Interaction among isolates and mycelial compatibility groups of Sclerotium cepivorum and cultivars of onion (Allium cepa). Can. J. Plant Pathol. 22:387-391. https://doi.org/10.1080/07060660009500457
  7. Entwistle, A. R. (1986) Loss of control of Allium white rot by fungicides and its implications. Aspects Appl. Biol. 40:166-175.
  8. Entwistle, A. R. (1990) Root diseases, In Onion and Allied crops, Vol. II; Rabinowitch, H. D. and Brewster, J. L. Eds: CRC Press, Boca Raton, USA. pp. 103-154.
  9. Groves, K. and K. S. Chough (1970) Fate of the fungicide 2,6- dichloro-4-nitroaniline (DCNA) in plants and soils. J. Agri. Food Chem. 18:1127-1128. https://doi.org/10.1021/jf60172a022
  10. Han, K. S., B. R. Kim, J. T. Kim, S. S. Hahm, K. H. Hong, C. K. Chung, Y. G. Nam, S. H. Yu and Jae Eul Choi (2013) Biological Control of white rot in garlic using Burkholderia pyrrocinia CAB08106-4. Res. Plant Dis. 19:21-24 https://doi.org/10.5423/RPD.2013.19.1.021
  11. Kim, C. H. (2001) Review of disease incidence of major crops in 2000. Korean J. Pesticide Sci. 5:1-11.
  12. Kim, C. H. (2002) Review of disease incidence of major crops in 2001. Res. Plant Dis. 8:1-10. https://doi.org/10.5423/RPD.2002.8.1.001
  13. Kim, C. H. (2004) Review of disease incidence of major crops in 2003. Res. Plant Dis. 10:1-7. https://doi.org/10.5423/RPD.2004.10.1.001
  14. Kim, Y. K., M. K. Kwon, H. S. Shim, T. S. Kim, W. H. Yeh, W. D. Cho, I. H. Choi, S. C. Lee, S. J. Ko, Y. H. Lee and C. J. Lee (2005) Various cultural factors associated with disease development of garlic white rot caused by two species of Sclerotium. Res. Plant Dis. 11:28-34. https://doi.org/10.5423/RPD.2005.11.1.028
  15. Lee, S. Y., S. B. Lee, Y. K. Kim and S. J. Hwang (2006) Biological control of garlic white rot accused by Sclerotium cepivorum and Sclerotium sp. using Bacillus subtilis 122 and Trichoderma harzianum 22. Res. Plant Dis. 12:81-84. https://doi.org/10.5423/RPD.2006.12.2.081
  16. Melero, J. M., R. Gonzalez, J. Gomez, J. Bejarano and M. J. Basallote (1989) Solarization of soils in Andalusia using plastics film. Plasticuiture 82:73-82.
  17. Melero-Vara, J. M., A. M. Prados-Ligero and M. J. Basallote- Ureba (2000) Comparison of physical, chemical and biological methods of controlling garlic white rot. European J. Plant Pathol. 106:581-588. https://doi.org/10.1023/A:1008777706814
  18. Pinto, C. M. F., L. A. Mafia, V. W. D. Casali, R. D. Berger and A. A. Cardoso (2000) Production components and yield losses of garlic cultivars planted at different times in a field naturally infested with Sclerotium cepivorum. Int. J. Pest Management 46:67-72. https://doi.org/10.1080/096708700227598
  19. Satour, M. M., M. F. Abdel-Rahim, T. Yamani, A. Grinstein, H. D. Rabinowitch and J. Katan (1989) Soil solarization in onion fields in Egypt and Israel: short- and long-term effects. Acta Hort. 255:151-159.
  20. Sommerville, P. A. and D. H. Hall (1987) Factors affecting sclerotia germination of Sclerotium cepivorum, secondary sclerotia formation, and germination stimulants to reduce inoculum density. Plant Dis. 71:229-233. https://doi.org/10.1094/PD-71-0229
  21. Zewde, T., C. Fininsa, P. K. Sakhuja and S. Ahmed (2007) Association of white rot (Sclerotium cepivorum) of garlic with environmental factors and cultural practices in the North Shewa highlands of Ethiopia. Crop Prot. 26:1566-1573. https://doi.org/10.1016/j.cropro.2007.01.007