Browse > Article
http://dx.doi.org/10.7585/kjps.2015.19.1.64

Control Activities of Fungicides Against Garlic White Rot Caused by Sclerotium cepivorum  

Kim, Heongjo (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
Kim, Heung Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
Min, Yi Gi (Department of Plant Medicine, College of Agriculture, Life and Environment Science, Chungbuk National University)
Publication Information
The Korean Journal of Pesticide Science / v.19, no.1, 2015 , pp. 64-70 More about this Journal
Abstract
In order to control garlic white rot (Sclerotium cepivorum), which threatens garlic production in farmers fields, soil solarization (solar sterilization), sclerotia germination inducers and effective microorganisms as biological control agents, and chemical fungicides have been used. Among them, fungicide has been largely used to reduce garlic white rot. In this study, the antifungal activities of five fungicides, prochloraz(a.i. 25%, EC), tebuconazole (a.i. 25%, WP), flutolanil (a.i. 15%, EC), iminoctadine tris-albesilate (a.i. 40%, WP) and isoprothiolane (a.i. 40%, EC) with different mode of action, in mycelial growth, sclerotia germination and sclerotia production, were tested. The inhibitory effects of the 5 fungicides on the mycelial growth, and sclerotia germination and production of garlic white rot pathogen (S. cepivorum T11-2) were investigated on potato dextrose agar (PDA) and their control efficacies were evaluated on garlic flakes. There was no mycelial growth of S. cepivorum T11-2 on PDA amended with $0.8{\mu}g\;mL^{-1}$ of prochloraz or $100{\mu}g\;mL^{-1}$ of tebuconazole. Also prochloraz and tebuconazole inhibited perfectively the sclerotia germination of the pathogen at 10 and $1.0{\mu}g\;mL^{-1}$, respectively. In spite of a very low activity of isoprothiolane in mycelial growth and sclerotia germination of S. cepivorum T11-2, it showed a good inhibitory activity against sclerotia production of S. cepivorum T11-2 on PDA amended with $1.67{\mu}g\;mL^{-1}$. Prochloraz, tebuconazole and flutolanil showed above 70% of control value when they were treated at $100{\mu}g\;mL^{-1}$ using the garlic flake cutting-method.
Keywords
Garlic white rot; Sclerotium cepivorum; Prochloraz; Tebuconazole; Isoprothiolane; inhibitory effect on sclerotia production; inhibitory effect on mycelial growth; inhibitory effect on sclerotia germination;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Andrea, T. B., Z. M. Emma, G. C. Carmen and F. C. Ronald (1996) The use of arbuscular mycorrhizae to control onion white rot (Sclerotium cepivorum Berk.) under field conditions. Mycorrhizae 6:253-257.   DOI
2 Cho W. D. and W. G. Kim (1996) Occurrence of white rot on Alliaceous vegetable crops. Korean J. Plant Pathol. 12:251-254.
3 Coley-Smith, J. R., C. M. Mitchell and D. E. Sansford (1990) Long-term survival of Sclerotium cepivorum and Stromatinia gladioli. Plant Pathology 39:58-69.   DOI
4 Coley-Smith, J. R. and J. E. King (1969) Production of volatile alkyl sulphides by microbial degradation of synthetic alliin and alliin-like compounds, in relation to germination of sclerotia of Sclerotium cepivorum Berk. Ann. Appl. Biol. 64:303-314.   DOI
5 Crowe, F. J., D. H. Hall, A. S. Greathead and K. G. Baghott (1980) Inoculum density of Sclerotium cepivorum and the incidence of white rot of onion and garlic. Phytopathology 70:64-69.   DOI
6 Earnshaw, D., M. R. McDonald and G. J. Boland (2000) Interaction among isolates and mycelial compatibility groups of Sclerotium cepivorum and cultivars of onion (Allium cepa). Can. J. Plant Pathol. 22:387-391.   DOI
7 Entwistle, A. R. (1986) Loss of control of Allium white rot by fungicides and its implications. Aspects Appl. Biol. 40:166-175.
8 Entwistle, A. R. (1990) Root diseases, In Onion and Allied crops, Vol. II; Rabinowitch, H. D. and Brewster, J. L. Eds: CRC Press, Boca Raton, USA. pp. 103-154.
9 Groves, K. and K. S. Chough (1970) Fate of the fungicide 2,6- dichloro-4-nitroaniline (DCNA) in plants and soils. J. Agri. Food Chem. 18:1127-1128.   DOI
10 Han, K. S., B. R. Kim, J. T. Kim, S. S. Hahm, K. H. Hong, C. K. Chung, Y. G. Nam, S. H. Yu and Jae Eul Choi (2013) Biological Control of white rot in garlic using Burkholderia pyrrocinia CAB08106-4. Res. Plant Dis. 19:21-24   DOI   ScienceOn
11 Kim, C. H. (2001) Review of disease incidence of major crops in 2000. Korean J. Pesticide Sci. 5:1-11.
12 Kim, C. H. (2002) Review of disease incidence of major crops in 2001. Res. Plant Dis. 8:1-10.   DOI
13 Kim, C. H. (2004) Review of disease incidence of major crops in 2003. Res. Plant Dis. 10:1-7.   DOI
14 Melero-Vara, J. M., A. M. Prados-Ligero and M. J. Basallote- Ureba (2000) Comparison of physical, chemical and biological methods of controlling garlic white rot. European J. Plant Pathol. 106:581-588.   DOI
15 Kim, Y. K., M. K. Kwon, H. S. Shim, T. S. Kim, W. H. Yeh, W. D. Cho, I. H. Choi, S. C. Lee, S. J. Ko, Y. H. Lee and C. J. Lee (2005) Various cultural factors associated with disease development of garlic white rot caused by two species of Sclerotium. Res. Plant Dis. 11:28-34.   DOI
16 Lee, S. Y., S. B. Lee, Y. K. Kim and S. J. Hwang (2006) Biological control of garlic white rot accused by Sclerotium cepivorum and Sclerotium sp. using Bacillus subtilis 122 and Trichoderma harzianum 22. Res. Plant Dis. 12:81-84.   DOI
17 Melero, J. M., R. Gonzalez, J. Gomez, J. Bejarano and M. J. Basallote (1989) Solarization of soils in Andalusia using plastics film. Plasticuiture 82:73-82.
18 Pinto, C. M. F., L. A. Mafia, V. W. D. Casali, R. D. Berger and A. A. Cardoso (2000) Production components and yield losses of garlic cultivars planted at different times in a field naturally infested with Sclerotium cepivorum. Int. J. Pest Management 46:67-72.   DOI
19 Satour, M. M., M. F. Abdel-Rahim, T. Yamani, A. Grinstein, H. D. Rabinowitch and J. Katan (1989) Soil solarization in onion fields in Egypt and Israel: short- and long-term effects. Acta Hort. 255:151-159.
20 Sommerville, P. A. and D. H. Hall (1987) Factors affecting sclerotia germination of Sclerotium cepivorum, secondary sclerotia formation, and germination stimulants to reduce inoculum density. Plant Dis. 71:229-233.   DOI
21 Zewde, T., C. Fininsa, P. K. Sakhuja and S. Ahmed (2007) Association of white rot (Sclerotium cepivorum) of garlic with environmental factors and cultural practices in the North Shewa highlands of Ethiopia. Crop Prot. 26:1566-1573.   DOI