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Evaluation of Antimicrobial Activity and Disease Control Efficacy of Sodium Dichloroisocyanurate (NaDCC) Against Major Strawberry Diseases

딸기 주요 병원균에 대한 친환경제제 NaDCC의 항균활성 및 병 방제효과 평가

  • Kim, Da-Ran (Division of Applied Life Science, Gyeongsang National University) ;
  • Gang, Gun-Hye (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, Hyun-Ji (Division of Applied Life Science, Gyeongsang National University) ;
  • Yoon, Hae-Suk (Gyeonsannam-do Agricultural Research and Extension Service) ;
  • Kwak, Youn-Sig (Division of Applied Life Science, Gyeongsang National University)
  • Received : 2015.01.15
  • Accepted : 2015.03.26
  • Published : 2015.03.31

Abstract

Various diseases occur in fruits, leave and roots during strawberry cultivation and cause severe economical damage and huge amount of chemical fungicide use. Recently, as consumers' interest in safety of foods and organic agriculture produces have increased, control measures using alternatives for chemical fungicides have been newly developed in various ways. This study was conducted to test antifungal activity and control effect of sodium dichloroisocyanurate (NaDCC), using as disinfectants, against major disease pathogens of strawberry, Fusarium oxysporum (Fusarium wilt), Colletotrichum gloeosporioides (Anthracnose) and Phytophthora sp. (Phytophthora blight), and Xanthomonas fragariae (bacterial angular leaf spot) and evaluate availability as environment-friendly materials. When NaDCC was treated at the concentration range of 150 to 300 ppm, it suppressed significantly hyphal growth and reduced spore germination by more than 28%. In field condition, NaDCC showed excellent control effect (control value: 50%) against the bacterial angular leaf spot disease. Based on above-described results, we suggested that NaDCC can be used as alternative candidates to chemical pesticide alternatives of for controlling strawberry diseases.

딸기에는 여러 가지 병이 발생하여 경제적으로 막대한 손실을 유발하며 많은 양의 농약이 사용되고 있는 실정이다. 최근 유기농산물과 식품안전성에 관한 소비자의 관심이 증가함에 따라 화학농약 대체제에 의한 병해 방제법 개발이 다각적으로 시도되고 있다. 본 연구에서는 광범위 소독제로 사용하고 있는 NaDCC의 딸기 주요 병원균(딸기 시들음병, 딸기 탄저병, 딸기 역병 그리고 딸기 세균성모무늬병)에 대한 항균활성 및 방제효과를 검증하고, 친환경제제로의 가능성을 확인하고자 수행되었다. NaDCC는 150~300 ppm 농도로 처리했을 때 병원진균의 균사생장을 효과적으로 억제하였고, 포자 발아율도 68% 이상 경감시키는 것으로 조사되었다. NaDCC는 딸기 세균성모무늬 병원균에 대하여서도 우수한 방제효과를 나타내었는데, 포장조건에서 딸기 세균성 모무늬병의 발생을 50% 감소시키는 것으로 나타났다. 이상의 결과를 토대로 NADCC는 딸기 병 방제용 방제제 후보물질로 사용될 수 있을 것으로 생각되었다.

Keywords

References

  1. Agrios, G. N. (1997). Plant Pathology. 4th ed. Academic Press, USA.
  2. Anandhakumar, J. and W. Zeller (2008) Biological control of red stele (Phytophthora fragariae var. fragariae) and crown rot (P. cactorum) disease of strawberry with rhizobacteria. J. of Plant Diseases and Protection. 115(2):49-56. https://doi.org/10.1007/BF03356238
  3. Kim, H., S. K. Heo, and J. E. Lee (2010) An analysis and implications on the consumption and consciousness situation of green consumers. Korean J. of Food Market. Eco. 27:43-62. (in Korean)
  4. Kim, H. J., S. H. Park, K. M. Cho and J. W. Kim (2007) Evaluation of time-dependent antimicrobial effect of sodium dichloroisocyanurate (NaDCC) on Enterococcus faecalis in the root canal. J. Korean Acad. Conserv. Dent. 32:121-129. (in Korean) https://doi.org/10.5395/JKACD.2007.32.2.121
  5. Kwon, J. H., H. S. Yoon, J. S. Kim, C. K. Shim and M. H. Nam (2010) Angular leaf spot of strawberry caused by Xanthomonas fragariae. Res. Plant Dis. 16:97-100. (in Korean) https://doi.org/10.5423/RPD.2010.16.1.097
  6. Lim, B. S., H. K. Yun, Y. S. Hwang, S. T. Choi and D. S. Chung (2003) Sterilization of cold storage room using sodium dichloroisocyanurate. J. Hort. Sci. Technol. 21, 209:211. (in Korean)
  7. Nam, M. H., H. S. Kim, W. K. Lee, M. L. Gleason and H. G. Kim (2011) Control efficacy of gray mold on strawberry fruits by timing of chemical and microbial fungicide applications. Kor. J. Hort. Sci. Technol. 29(2):151.
  8. Nam, M. H., H. S. Kim, Y.G. Nam, N. A. Peres and H. G. Kim (2011) Fungicide spray program to reduce application in anthracnose of strawberry. J. Plant Pathol, 17(3):295-301.
  9. Nam, M. H., S. K. Jung, S. J. Yoo, S. S. Kwan and H. G. Kim (2011) Cultural and pathogenic characteristics between Colletotrichum gloeosporioides and Glomerella cingulata isolated from strawberry in Korea. J. Plant Pathol. 14:654-655.
  10. Mass, J. L. (1998) Compendium of Strawberry Diseases. 2nd edition. APS press, USA.
  11. Matuo, T., H. Komada and A. Matsuda (1980) Fusarium disease of cultivated plants. Zenkoku Noson Kyoiku Kyoiku Publishing Co. Tokyo, Japan. Pp. 201-220. (in Japanese)
  12. Niedz, R. P., and Bausher, M. G. (2002) Control of in vitro contamination of explants from greenhouse and field grown trees. In Vitro Cell Dev. Biol. Plant 38:468-471. https://doi.org/10.1079/IVP2002316
  13. Soh, J. W., K. S. Han, S. C. Lee, J. H. Park, and K. N. Kim (2014) Control effects of environment-friendly materials on anthracnose pathogen in red pepper. Korean J. Int. Agric., 26:162-168. (in Korean) https://doi.org/10.12719/KSIA.2014.26.2.162
  14. Song, J. H., S. H. Roh, Y. H. Jeong, J. H. Ha, and B. J. Moon (1998) Occurrence of Phytophthora rot of strawberry caused by Phytophthora nicotianae var. nicotianae. Korean J. Plant pathol. 14:445-451. (in Korean)
  15. Clasen, T. and P. Edmondson (2006) Sodium dichloroisocyanurate (NaDCC) tablets as an alternative to sodium hypochlorite for the routine treatment of drinking water at the household level. Int. J. Hyg. Environ. Health 209:173-181. https://doi.org/10.1016/j.ijheh.2005.11.004
  16. Yoon, D. H., K. W. Nam, M. K. Han and H. J. Park (2009) Control effect of NaDCC on the abnormal-skin-stain during storage in Niitaka'pears. Kor. J. Hort. Sci. Technol. 27 (Suppl. I).

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