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Sample Preparation Method for Perfluorochemicals with LC-Tandem Mass Spectrometry in Agricultural Water

LC-Tandem Mass Spectrometry를 활용한 농업용수 중 Perfluorochemicals 분석시료 전처리법

  • Kim, Jin Hyo (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Jin, Cho-Long (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Geun-Hyoung (Chemical Safety Division, National Academy of Agricultural Science, RDA) ;
  • Park, Byung-Jun (Chemical Safety Division, National Academy of Agricultural Science, RDA)
  • 김진효 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 진초롱 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 최근형 (농촌진흥청 국립농업과학원 화학물질안전과) ;
  • 박병준 (농촌진흥청 국립농업과학원 화학물질안전과)
  • Received : 2014.11.26
  • Accepted : 2015.03.26
  • Published : 2015.03.31

Abstract

The current official perfluorochemicals (PFCs) analysis method is established with hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) clean-up method with LC-tandem mass spectrometry ($MS^n$). Herein the HLB clean-up method from the official method by Korean government was modified with Envi-Carb$^{TM}$ to improve the clean-up efficiency for agricultural water and the unexpected UV disturbance in the water was effectively removed by the adsorbent treatment without a significant disturbance of quantitation. And quadrupole time of flight mass spectrometry (qTOFMS) was suggested to the available $MS^n$ instruments for the residue analysis of PFCs based on the ng/L of quantitation limits in water as well.

잔류성 유기오염물질인 과불화합물 중 perfluorooctanesufonic acid (PFOS)와 perfluorooctanoic acid (PFOA)의 잔류분석은 환경부 "잔류성 유기오염물질 공정시험기준"에 따라 hydrophilic-lipophilic balance (HLB) solid phase extraction (SPE) 전처리 후 LC-$MS^n$를 이용해 정량 분석하고 있다. 본 연구에서는 환경부 설정 공정시험법에 따라 농업용수 전처리 후, 시험법에서 제거하지 못한 미지의 불순물을 Envi-Carb$^{TM}$을 통해 회수율에 영향을 주지 않고 손쉽게 제거하였다. 또한, 과불화합물 분석에 사용되는 LC-$MS^n$ 중 quadrupole-time-of-flight mass spectrometry (qTOFMS)에서 측정된 PFCs의 정량한계를 평가한 결과, 시험에 사용된 장비간 정량한계 편차가 크게 관찰되었으나, 시험대상 장비 모두 농업용수 중 ng/L 수준으로 잔류하는 과불화합물의 정밀 잔류분석에 사용가능 한 것으로 확인되었다.

Keywords

References

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