한국버섯학회지 (Journal of Mushroom)

  • 제13권1호
  • /
  • Pages.30-36
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  • 2015
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
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아위버섯 추출물의 혈전용해, 트롬빈저해, 항산화 및 항염증 활성

Fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of Pleurotus ferulea

  • 김은정 (상지대학교 임상병리학과) ;
  • 김준호 (상지대학교 정밀화학신소재학과)
  • Kim, Eun-Jung (Department of Biomedical Laboratory Science, Sangji University) ;
  • Kim, Jun-Ho (Department of Fine Chemistry and New Materials, Sangji University)
  • 투고 : 2015.02.12
  • 심사 : 2015.03.20
  • 발행 : 2015.03.31
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초록

아위버섯을 혈관계질환 예방과 치료를 위한 기능성 식품 개발에 이용하기 위한 기초 자료를 얻기 위해 아위버섯 물추출물과 유기용매 분획물의 혈전용해활성과 트롬빈 저해활성, 항산화활성 및 항염증활성을 확인하였다. 에틸아세테이트 분획물은 1.33 plasmin unit의 높은 혈전용해 활성과, 94.45%의 높은 트롬빈 저해활성을 나타냈으며, 물 추출물은 37.01%의 항산화활성을 나타냈으며, 클로로포름 분획물은 98.13%의 가장 높은 항염증활성을 나타냈다. 실험 결과로부터 아위버섯의 에틸아세테이트 분획물은 높은 혈전용해활성, 트롬빈 저해활성 그리고 클로로포름 분획물은 높은 항염증활성을 나타내 심혈관계 질환 관련 치료나 예방을 위한 기능성식품 개발에 이용 가능할 것으로 기대된다.

Our study investigated the fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of the water extract and solvent fractions isolated from Pleurotus ferulea. Fibrinolytic activity was investigated using the fibrin plate method. Thrombin inhibitory activity was used to analyze thrombin inhibitor assay. The DPPH assay was used to estimate anti-oxidative activity. Inhibition of NO production was measured for anti-inflammatory activity in LPS-activated murine RAW 264.7 macrophage cells. An MTS assay was used to evaluate the effects of the water extract and solvent fractions isolated from Pleurotus ferulea on cell viability. Our results showed the fibrinolytic activity to be strong in the ethyl acetate fraction at 1.33 plasmin units. The ethyl acetate fraction also showed high thrombin inhibitory activity at 94.45%. The anti-oxidative activity of the water extract was 37.01% and the anti-inflammatory activity of the chloroform fraction was 98.13%. These findings suggest that Pleurotus ferulea's extract and fractions could be applicable in the development of functional foods for the treatment and prevention of cardiovascular diseases.

키워드

참고문헌

  1. Blois MS. 1958. Antioxidant determinations by the use of a stale free radical. Nature. 181:1199-1120. https://doi.org/10.1038/1811199a0
  2. Chihara G, Hamuro J, Maeda YY, Arai Y, Fukuoka F. 1970. Fractionation and purification of the polysaccharide with marked antitumor activity, especially lentinan from Lentinus edodes. Cancer Res. 30:2776-2781.
  3. Choi JH, Ha TM, Kim YH, Rho YD. 1996. Studies on the main factors affecting the mycelial growth of Phellinus lintenus. Kor J Mycol. 24:214-222. (in Korean)
  4. Choi SJ, Lee YS, Kim JK, Kim JK, Lim SS. 2010. Physiological activities of extract from edible mushrooms. J Kor Soc Food Sci Nutr. 39(8):1087-1096. (in Korean) https://doi.org/10.3746/jkfn.2010.39.8.1087
  5. Doljak B, Stegnar M, Urleb U, Kreft S, Umek A, Ciglaric M, Strukelj B. Popovic T. 2001. Screening for selective thrombin inhibitor in mushrooms. Blood Coagul Fibrinolysis. 12:123-128. https://doi.org/10.1097/00001721-200103000-00006
  6. Haverkate F, Traas DW. 1974. Dose-response curves in the fibrin plate assay. Fibrinolytic activity of protease. Thromb Haemost. 32:356-365.
  7. Hong KH, Kim BY, Kim HK. 2004a. Analysis of nutritional components in Pleurotus ferulea. Kor J Food Sci Technol. 36(4):563-567. (in Korean)
  8. Hong KH, Kim BY, Kim HK. 2004b. Studies on the biological activity of Pleurotus ferulea. J Kor Soc Food Sci Nutr. 33(5):791-796. (in Korean) https://doi.org/10.3746/jkfn.2004.33.5.791
  9. Hyun KW, Jeong SC, Lee DH, Park JS, Lee JS. 2006. Isolation and characterization of a noble platelet aggregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus. Peptides. 27:1173-1178. https://doi.org/10.1016/j.peptides.2005.10.005
  10. Kang MG, Bolormaa Z, Lee JS, Seo GS, Lee JS. 2011. Antihypertensive activity and anti-gout activity of mushroom Sarcodon aspratus. Kor J Mycol. 39(1):53-56. (in Korean) https://doi.org/10.4489/KJM.2011.39.1.053
  11. Kim JH. 2011. Physiological activities of hot water extract and solvent fractions of Pleurotus ferulea. Kor J Mycol. 39:189-193. (in Korean) https://doi.org/10.4489/KJM.2010.39.3.189
  12. Kim JH. 2014. Physiological activities of water extract and solvent fractions of an edible mushroom, Pholiota adiposa. Kor J Mycol. 42:207-212. (in Korean) https://doi.org/10.4489/KJM.2014.42.3.207
  13. Kim JH, Kim YS. 1999. A fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea. Biosci Biotech Biochem. 63(12):2130 -2136. https://doi.org/10.1271/bbb.63.2130
  14. Kim YJ, Kim CK, Kwon YJ. 1997. Isolation of antioxidative components of Perillae semen. Kor J Food Sci Technol. 29(1):38-43. (in Korean)
  15. Oh HS, Kim JH. 2007. Physiological functionalities of hot water extract of Codonopsis lanceolata and some medicinal materials, and their mixtures. Korean J Community Living Sci. 18:407-415. (in Korean)
  16. Shin HH, Choi HS. 1998. Purification and partial characterization of a Metalloprotease in Flammulina velutipes. J Microbiol. 36:20-25.
  17. Tsukagoshi S, Ohashi F. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and rat ascites hepatoma AH-13 by oral use. Gann. 65:557-558.

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  2. Anti-inflammatory properties of chloroform extracts from GW10-45, a new cultivar derived from Pleurotus ferulae, in RAW264.7 murine macrophages vol.14, pp.4, 2016, https://doi.org/10.14480/JM.2016.14.4.220
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