Browse > Article
http://dx.doi.org/10.14480/JM.2015.13.1.30

Fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of Pleurotus ferulea  

Kim, Eun-Jung (Department of Biomedical Laboratory Science, Sangji University)
Kim, Jun-Ho (Department of Fine Chemistry and New Materials, Sangji University)
Publication Information
Journal of Mushroom / v.13, no.1, 2015 , pp. 30-36 More about this Journal
Abstract
Our study investigated the fibrinolytic, thrombin inhibitory, anti-oxidative and anti-inflammatory activities of the water extract and solvent fractions isolated from Pleurotus ferulea. Fibrinolytic activity was investigated using the fibrin plate method. Thrombin inhibitory activity was used to analyze thrombin inhibitor assay. The DPPH assay was used to estimate anti-oxidative activity. Inhibition of NO production was measured for anti-inflammatory activity in LPS-activated murine RAW 264.7 macrophage cells. An MTS assay was used to evaluate the effects of the water extract and solvent fractions isolated from Pleurotus ferulea on cell viability. Our results showed the fibrinolytic activity to be strong in the ethyl acetate fraction at 1.33 plasmin units. The ethyl acetate fraction also showed high thrombin inhibitory activity at 94.45%. The anti-oxidative activity of the water extract was 37.01% and the anti-inflammatory activity of the chloroform fraction was 98.13%. These findings suggest that Pleurotus ferulea's extract and fractions could be applicable in the development of functional foods for the treatment and prevention of cardiovascular diseases.
Keywords
Pleurotus ferulea; Fibrinolytic activity; Thrombin inhibitory activity; Anti-oxidative activity; Anti-inflammatory activity;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Blois MS. 1958. Antioxidant determinations by the use of a stale free radical. Nature. 181:1199-1120.   DOI   ScienceOn
2 Chihara G, Hamuro J, Maeda YY, Arai Y, Fukuoka F. 1970. Fractionation and purification of the polysaccharide with marked antitumor activity, especially lentinan from Lentinus edodes. Cancer Res. 30:2776-2781.
3 Choi JH, Ha TM, Kim YH, Rho YD. 1996. Studies on the main factors affecting the mycelial growth of Phellinus lintenus. Kor J Mycol. 24:214-222. (in Korean)
4 Choi SJ, Lee YS, Kim JK, Kim JK, Lim SS. 2010. Physiological activities of extract from edible mushrooms. J Kor Soc Food Sci Nutr. 39(8):1087-1096. (in Korean)   DOI   ScienceOn
5 Doljak B, Stegnar M, Urleb U, Kreft S, Umek A, Ciglaric M, Strukelj B. Popovic T. 2001. Screening for selective thrombin inhibitor in mushrooms. Blood Coagul Fibrinolysis. 12:123-128.   DOI   ScienceOn
6 Haverkate F, Traas DW. 1974. Dose-response curves in the fibrin plate assay. Fibrinolytic activity of protease. Thromb Haemost. 32:356-365.
7 Hong KH, Kim BY, Kim HK. 2004a. Analysis of nutritional components in Pleurotus ferulea. Kor J Food Sci Technol. 36(4):563-567. (in Korean)
8 Hong KH, Kim BY, Kim HK. 2004b. Studies on the biological activity of Pleurotus ferulea. J Kor Soc Food Sci Nutr. 33(5):791-796. (in Korean)   DOI   ScienceOn
9 Hyun KW, Jeong SC, Lee DH, Park JS, Lee JS. 2006. Isolation and characterization of a noble platelet aggregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus. Peptides. 27:1173-1178.   DOI   ScienceOn
10 Kang MG, Bolormaa Z, Lee JS, Seo GS, Lee JS. 2011. Antihypertensive activity and anti-gout activity of mushroom Sarcodon aspratus. Kor J Mycol. 39(1):53-56. (in Korean)   DOI   ScienceOn
11 Kim JH. 2011. Physiological activities of hot water extract and solvent fractions of Pleurotus ferulea. Kor J Mycol. 39:189-193. (in Korean)   DOI   ScienceOn
12 Kim JH. 2014. Physiological activities of water extract and solvent fractions of an edible mushroom, Pholiota adiposa. Kor J Mycol. 42:207-212. (in Korean)   DOI   ScienceOn
13 Kim JH, Kim YS. 1999. A fibrinolytic metalloprotease from the fruiting bodies of an edible mushroom, Armillariella mellea. Biosci Biotech Biochem. 63(12):2130 -2136.   DOI   ScienceOn
14 Kim YJ, Kim CK, Kwon YJ. 1997. Isolation of antioxidative components of Perillae semen. Kor J Food Sci Technol. 29(1):38-43. (in Korean)
15 Oh HS, Kim JH. 2007. Physiological functionalities of hot water extract of Codonopsis lanceolata and some medicinal materials, and their mixtures. Korean J Community Living Sci. 18:407-415. (in Korean)
16 Shin HH, Choi HS. 1998. Purification and partial characterization of a Metalloprotease in Flammulina velutipes. J Microbiol. 36:20-25.
17 Tsukagoshi S, Ohashi F. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma-180 and rat ascites hepatoma AH-13 by oral use. Gann. 65:557-558.