Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Occurrence of a Natural Hybrid between Acheilognathus signifer and A. lanceolatus (Pisces: Cyprinidae)

묵납자루 Acheilognathus signifer와 납자루 A. lanceolatus 사이의 자연 잡종 출현

  • Kim, Hyeong Su (Department of Biological Science and Institute for Biodiversity, College of Natural Sciences, Chonbuk National University) ;
  • Yun, Seung Woon (Department of Biological Science and Institute for Biodiversity, College of Natural Sciences, Chonbuk National University) ;
  • Kim, Hyun Tae (Department of Biological Science and Institute for Biodiversity, College of Natural Sciences, Chonbuk National University) ;
  • Park, Jong Young (Department of Biological Science and Institute for Biodiversity, College of Natural Sciences, Chonbuk National University)
  • 김형수 (전북대학교 자연과학대학 생물학과, 부설 생물다양성연구소) ;
  • 윤승운 (전북대학교 자연과학대학 생물학과, 부설 생물다양성연구소) ;
  • 김현태 (전북대학교 자연과학대학 생물학과, 부설 생물다양성연구소) ;
  • 박종영 (전북대학교 자연과학대학 생물학과, 부설 생물다양성연구소)
  • Received : 2015.09.10
  • Accepted : 2015.09.25
  • Published : 2015.09.30
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Abstract

A natural hybrid between Acheilognathus signifer and A. lanceolatus was collected in their sympatric area, the Gimhwanamdaecheon of Hangang (River), Korea. Morphological characters, mitochondrial cytochrome b gene (cyt b), and recombination-activating gene 1 (RAG-1) were investigated to confirm the natural hybrid origin. As a result of morphological characters, the natural hybrid was appeared to have intermediate characters between two parental species in three characters; the band of dorsal fin, the color patterns of anal fin membrane, and the body color. In analysis of cyt b, it was revealed that the maternal species of the natural hybrid was appeared to be A. signifer due to their 99.9% sequence identity. Also, in analysis of RAG-1, an electropherogram of the hybrid individual displayed double peaks, strongly indicating its hybrid state.

묵납자루 Acheilognathus signifer와 납자루 A. lanceolatus간 잡종으로 추정되는 개체를 한강 수계의 김화남대천에서 채집하였다. 잡종의 기원을 확인하기 위하여 형태적 특징과 미토콘드리아 cytochrome b gene (cyt b)와 recombination-activating gene 1 (RAG-1)를 분석하였다. 외부 형태를 분석한 결과 잡종 개체는 등지느러미와 뒷지느러미 반문, 뒷지느러미 색깔, 몸의 체색 등은 두 부모종의 중간적 형질을 보였다. 미토콘드리아 cyt b 분석 결과 잡종 개체는 묵납자루와 염기서열이 99.9% 일치하여 묵납자루가 모계임이 밝혀졌다. 또한 RAG-1 분석 결과 double peak가 나타났는데 이는 잡종 개체임을 강력하게 시사하였다.

Keywords

References

  1. Allendorf, F.W., R.F. Leary, P. Spruell and J.K. Wenburg. 2001. The problems with hybrids: setting conservation guidelines. Trends. Ecol. Evol., 16: 613-622. https://doi.org/10.1016/S0169-5347(01)02290-X
  2. Avise, J.C. and N.C. Saunders. 1984. Hybridization and introgression among species of sunfish (Lepomis): analysis by mitochondrial DNA and allozyme markers. Genetics, 108: 237-255.
  3. Campton, D.E. 1987. Natural hybridization and introgression in fishes: Methods of detection and genetic interpretations. In: Ryman, N. and F. Utter (eds.), Population Genetics and Fishery Management. University of Washington Press, Seattle, WA, USA, pp. 161-192.
  4. Damme, V.D., N. Bogutskaya, R.C. Hoffimann and C. Smith. 2007. The introduction of the European bitterling (Rhodeus amarus) to west and central Europe. Fish and Fisheries, 8: 79-106. https://doi.org/10.1111/j.1467-2679.2007.00239.x
  5. Gerber, A.S., C.A. Tibbets and T.E. Dowling. 2001. The role of introgressive hybridization in the evolution of the Gila robusta complex (Teleostei: Cyprinidae). Evolution, 55: 2028-2039. https://doi.org/10.1111/j.0014-3820.2001.tb01319.x
  6. Grant, P.R. and B.R. Grant. 1992. Hybridization of bird species. Science, 256: 193-197. https://doi.org/10.1126/science.256.5054.193
  7. Hubbs, C.L. 1955. Hybridization between fish species in nature. Syst. Zool., 4: 1-20. https://doi.org/10.2307/2411933
  8. Hubbs, C.L., K.F. Lagler and G.R. Smith. 2004. Fishes of the great lakes region. The Univ. Michigan Press, 250pp.
  9. Hwang, Y.J., M.S. Ra and C.G. Choi. 1995. A natural hybrid between spinous loach, Cobitis longicorpus and cyprinid loach Misgurunus anguilicaudatus (Pisces, Cobitidae). Korean J. Ichthyol., 7: 203-207. (in Korean)
  10. Jansson, H., I. Holmgren, K. Wedin and T. Andersson. 1991. High frequency of natural hybrids between Atlantic salmon, Salmo salar L., and brown trout, S. trutta L., in a Swedish river. J. Fish Biol., 39: 343-348. https://doi.org/10.1111/j.1095-8649.1991.tb05096.x
  11. Kim, C.H., W.O. Lee, Y.J. Kang and J.M. Baek. 2010. Occurrence of a natural intergeneric hybrid, Rhodeus uyekii${\times}$Acheilognathus signifer (Pisces; Cyprinidae) from Jojongcheon Bukhan River, Korea. Korean J. Ichthyol., 22: 225-229. (in Korean)
  12. Kim, D.M., H.B. Jeon and H.Y. Suk. 2014a. Tanakia latimarginata, a new species of bitterling from the Nakdong River, South Korea (Teleostei: Cyprinidae). Ichthyol. Explor. Freshwaters, 25: 59-68.
  13. Kim, H.S., S.W. Yun, J.G. Ko and J.Y. Park. 2014b. Occurrence of a natural intergeneric hybrid between Rhodeus pseudosericeus and Acheilognathus signifer (Pisces: Cyprinidae) from the Namhangang (river), Korea. Korean J. Ichthyol., 26: 153-158.
  14. Kim, I.S. 1997. Illustrated encyclopedia of fauna and flora of Korea. VoL. 37. Freshwater fishes. Ministry of Education, 629pp. (in Korean)
  15. Kim, I.S. and J.H. Lee. 1990. Diploid-triploid hybrid complex of the spined loach Cobitis sinensis and C. longicorpus (Pisces, Cobitidae). Korean J. Ichthyol., 2: 203-210.
  16. Kim, I.S., Y. Choi and J.H. Shim. 1991. An occurrence of intergeneric hybrid cross, Pungtungia herzi${\times}$Pseudopungtungia nigra from the Ungcheon River, Korea. Korean J. Ichthyol., 3: 42-47.
  17. Kim, I.S. and J.Y. Park. 2002. Freshwater fishes of Korea. Kyohak publishing, 466pp. (in Korean)
  18. Kim, K.Y., M.H. Ko, S.J. Cho, W.J. Kim, M.H. Son and I.C. Bang. 2015. A natural hybrid of intergeneric mating between a female Pungtungia herzi and a male Pseudorasbora parva (Cypriniformes: Cyprinidae). Fish. Aquat. Sci., 18: 99-107.
  19. Lee, I.R., H. Yang, J.H. Kim, K.Y. Kim and I.C. Bang. 2009. Identification of a natural hybrid between the striped spine loach Cobitis tetralineata and the king spine loach Iksookimia longicorpa by analyzing mitochondrial COI and nuclear RAG-1 sequences. Korean J. Ichthyol., 21: 287-290. (in Korean)
  20. Nelson, J.S. 2006. Fishes of the world (4th ed). John Wiley and Sons, Inc., 601pp.
  21. Nikoljukin, M.J. 1972. Distant hybridization in acipenseridae and teleostei. Moskava, 335pp.
  22. Okazaki, M., K. Narus, A. Shima and R. Arai. 2001. Phylogenetic relationships of bitterlings based on mitochondrial 12S ribosomal DNA sequences. J. Fish Biol., 58: 89-106. https://doi.org/10.1111/j.1095-8649.2001.tb00501.x
  23. Sonnenberg, R., A. Nolte and D. Tautz. 2007. An evaluation of LSU rDNA D1-D2 sequences for their use in species identification. Front. Zool., 4: 6. https://doi.org/10.1186/1742-9994-4-6
  24. Soric V.M. 2004. A natural hybrid of Leuciscus cephalus and Alburnus alburnus (Pisces, Cyprinidae) from the Ibar River (Western Serbia). Arch. Biol. Sci., Belgrade, 56: 23-32. https://doi.org/10.2298/ABS0402023S
  25. Yun, Y.E., I.R. Lee, S.Y. Park, E.J. Kang, E.O. Kim, S.K. Yang, Y.K. Nam and I.C. Bang. 2009. Genetic identification of hybrids between Rhodeus uyekii and R. notatus by sequence analysis of RAG-1 gene. J. Aquaculture, 22: 79-82. (in Korean)