Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation of Antimicrobial Active Substance from Aristolochia tagala Champ. against Sclerotial Rot (Sclerotinia sclerotiorum)

이엽마두령(Aristolochia tagala Champ.)추출물로부터 균핵병 병원균(Sclerotinia sclerotiorum)에 대한 항균 활성물질 탐색

  • 김현상 (경북대학교 대학원 생태환경시스템학과) ;
  • 손진한 (한국한방산업진흥원 한방제품개발팀) ;
  • 최용화 (경북대학교 생태환경대학 생태환경시스템학부)
  • Received : 2015.11.06
  • Accepted : 2015.11.16
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Aristolochia tagala Champ. was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by column chromatography and obtained forty three subfractions. The forty three fractions were searched the anti-fungal activities by bioassay. The most active No. 26 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, 2,4-di-tetra-butyl-phenol, 2-mono-palmitin, 1-mono-stearin were profiled as maine compounds in No. 26 subfraction. Bioassay using commercial 1-mono-stearin to test for the anti-microbial activity conformed the antimicrobial active compound. In conclusion, 1-mono-stearin identified from Aristolochia tagala Champ. was antimicrobial chemical against Sclerotinia sclerotiorum.

본 연구는 균핵병의 원인균인 Sclerotinia sclerotiorum에 대해 항균활성을 갖는 친환경 유기농자재를 개발할 목적으로 이엽마두령(Aristolochia tagala Champ.)으로부터 활성물질 분리하였다. 이엽마두령을 MeOH로 추출하여 용매분획을 하였고, 용매분획 중에서 가장 강한 활성을 나타낸 $CHCl_3$ fraction을 column chromatography로 분리하여 43개의 subfraction을 얻었다. 43개의 subfraction을 bioassay한 결과 MYHS26 subfraction에서 강한 활성을 나타내었다. MYHS26 subfraction을 GC-MS로 분석하여 chromatogram 상의 주요 peak에 해당하는 mass spectrum과 Wiley library를 비교하여 profiling한 결과, 2,4-di-tetra-butyl-phenol, 2-monopalmitin, 1-mono-stearin이 주요 물질로 검출되었다. 항균활성물질로 추정되는 1-mono-stearin 화합물의 항균활성을 검정하기 위하여 표준품을 사용하여 생물검정한 결과 이 화합물이 강한 활성을 나타내었다. 따라서 이엽마두령으로부터 분리한 1-mono-stearin 화합물이 균핵병의 원인균인 Sclerotinia sclerotiorum에 대한 항균 활성물질인 것으로 추정되었다.

Keywords

References

  1. Abawi, G. S. and Grogan, R. G. 1975. Source of primary inoculum and effects of temperature and moisture on infection of beans by Whetzelinia sclerotiorum. Phytopathology. 65: 300-309. https://doi.org/10.1094/Phyto-65-300
  2. Abawi, G. S. and Grogan, R. G. 1979. Epidemiology of disease caused by Sclerotinia species. Phytopathology. 69: 899-904 https://doi.org/10.1094/Phyto-69-899
  3. Agrios, G. N. 1998. Plant pathology. 4rd ed., Academic Press. p. 635.
  4. Bardin, S. D. and H. C. Huang. 2001. Research on biology and control of Sclerotinia diseases in Canada. Can. J. Plant Pathol. 23: 88-98. https://doi.org/10.1080/07060660109506914
  5. Baskar K., S. Sasikumar, C. Muthu, S. Kingsley, and S. Ignacimuthu. 2011. Bioefficacy of Aristolochia tagala Cham. against Spodoptera litura Fab. (Lepidoptera: Noctuidae). Saudi Journal of Biological Sciences. 18: 23-27 https://doi.org/10.1016/j.sjbs.2010.09.004
  6. Boland, G. J. and R. Hall. 1994. Index of host plants of Sclerotinia sclerotiorum. Canadian journal of Plant Pathol. 16: 93-108. https://doi.org/10.1080/07060669409500766
  7. Chang, S. W. and S. K. Kim. 2003. First report of Sclerotinia rot caused by Sclerotinia sclerotiorumon some vegetable crops in Korea. Plant Pathology J. 19: 79-84. https://doi.org/10.5423/PPJ.2003.19.2.079
  8. Cho, C. T. 1976. Present Status and Problems for the Control of Vegetable Diseases in the Vinyl house. Korean J. Plant Protection. 154: 215-222
  9. Hall, B. H., R. L. McMahon, and T. J. Wicks. 2002. First report of Sclerotinia sclerotiorumon grape (Vitis vinifera) in South Australia. Australasian Plant Pathol. 31: 417-418. https://doi.org/10.1071/AP02045
  10. Hong, S. K., W. G. Kim, G. B. Sung, S. H. Nam, and J. S. Kim. 2007. Aspects of Popcorn Disease Occurrence on Mulberry Fruits in Korea. Res. Plant Dis. 13(3): 131-136 https://doi.org/10.5423/RPD.2007.13.3.131
  11. Hwang, J. Y., C. K. Shim, K. Y. Ryu, D. H. Choi, and H. J. Jee. 2006. Selection of Brevibacillus brevis B23 and Bacillus stearothermophilus B42 as biological control agents against sclerotinia rot of lettuce. Res. Plant Dis. 12: 254-259. https://doi.org/10.5423/RPD.2006.12.3.254
  12. Kang, J. Y., D. H. Kim, D. G. Lee, I. S. Kim, M. G. Jeon, J. D. Lee, I. H. Kim, and Sanghyun Lee. 2013. Screening of Antifungal Activities of Medicinal Plants for the Control of Turfgrass Fungal Disease. Weed Turf. Sci. 2(1): 70-75 https://doi.org/10.5660/WTS.2013.2.1.070
  13. Kim, H. W., K. Y. Lee, J. W. Baek, H. J. Kim, J. Y. Park, J. W. Lee, S. J. Jung, and B. J. Moon. 2004. Isolationj and identification of antagonistic bacterium active against Sclerotinia sclerotiorum causing sclerotinia rot on crisphead lettuce. Res. Plant Dis. 12: 254-259.
  14. Kim, K. C. 1976. The effect of ray on sclerotia formation of sclerotium disease. Korean J. Plant Protect. 15: 223-243.
  15. Kim, W. G., W. D. Cho, and H. J. Jee, 1999. Occurrence of Sclerotinia rot on cucurbitaceous vegetable crops in greenhouses. Korean J. Mycol. 27: 198-205
  16. Kim, W. G. and W. D. Cho. 2002. Occurrence of Sclerotinia rot on composite vegetable crops and the causal Sclerotinia spp. Mycobiology. 30: 41-46. https://doi.org/10.4489/MYCO.2002.30.1.041
  17. Kim, W. G. and W. D. Cho. 2003. Occurrence of Sclerotinia rot in cruciferous crops caused by Sclerotinia spp. Plant Pathol. J. 19: 69-74 https://doi.org/10.5423/PPJ.2003.19.1.069
  18. Kim, W. G., S. K. Hong, and S. Y. Lee. 2006. Occurrence of Sclerotinia rot in four leguminous crops caused by Sclerotinia sclerotiorum. Plant Pathol. J. 22: 16-20 https://doi.org/10.5423/PPJ.2006.22.1.016
  19. Kora, C., M. R. McDonald, and G. J. Boland. 2003. Sclerotinia rot of carrot: An example of phenological adaptation and bicyclic development by Sclerotinia sclerotiorum. plant Dis. 87: 456-470. https://doi.org/10.1094/PDIS.2003.87.5.456
  20. The Korean Society Of Plant Pathology. 2004. List of Plant Diseases in Korea. p. 779.
  21. Latorre, B. A. and M. J. Guerrero. 2001. First report of shoot blight of grapevine caused by Sclerotinia sclerotiorum in Chile. Plant Dis. 85: 1122.
  22. Purdy, L. H. 1979. Sclerotinia sclerotiorum: History, diseases and symptomatology, host range, geographic distribution, and impact. Phytopathology 69: 875-880. https://doi.org/10.1094/Phyto-69-875
  23. Schwartz, H. F. and J. R. Steadman. 1978. Factors affecting sclerotium populations of, and apothecium production by Sclerotinia sclerotiorum. phytopathology 68: 383-388. https://doi.org/10.1094/Phyto-68-383
  24. Shin, D. B. and J. T. Lee. 1987. Ecological studies on lettuce drop disease occurring under controlled cultivation conditions in drained paddy fields. Korean J. Plant Pathol. 3: 252-260
  25. Subbarao, K. V. 1998. Progress toward integrated management of lettuce drop. Plant Dis. 82: 1068-1078. https://doi.org/10.1094/PDIS.1998.82.10.1068
  26. Thirugnanasampandan R., G. Mahendran, and V. Narmatha Bai. Antioxidant properties of some medicinal Aristolochiaceae species. African Journal of Biotechnology. 7(4): 357-361.
  27. Whipps, J. M., S. P. Budge, S. McClement, and D. A. C. Pink. 2002. A glasshouse cropping method for screening lettuce lines for resistance to Sclerotinia sclerotiorum. Eur. J. Plant Pathol. 108: 373-378. https://doi.org/10.1023/A:1015637018474