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http://dx.doi.org/10.11625/KJOA.2015.23.4.951

Isolation of Antimicrobial Active Substance from Aristolochia tagala Champ. against Sclerotial Rot (Sclerotinia sclerotiorum)  

Kim, Hyun-Sang (경북대학교 대학원 생태환경시스템학과)
Shon, Jinhan (한국한방산업진흥원 한방제품개발팀)
Choi, Yong-Hwa (경북대학교 생태환경대학 생태환경시스템학부)
Publication Information
Korean Journal of Organic Agriculture / v.23, no.4, 2015 , pp. 951-962 More about this Journal
Abstract
To develop environment-friendly agricultural products with anti-microbial activity against Sclerotinia sclerotiorum as a pathogen of sclerotium disease, Aristolochia tagala Champ. was extracted by methanol and its extract was fractionated into several solvent fractions. The chloroform fraction, which showed the highest antimicrobial activity, was separated by column chromatography and obtained forty three subfractions. The forty three fractions were searched the anti-fungal activities by bioassay. The most active No. 26 subfraction was analyzed by GC-MS. Each mass spectra, corresponding to each peak of chromatogram, was compared to MS database of Wiley library. As a result, 2,4-di-tetra-butyl-phenol, 2-mono-palmitin, 1-mono-stearin were profiled as maine compounds in No. 26 subfraction. Bioassay using commercial 1-mono-stearin to test for the anti-microbial activity conformed the antimicrobial active compound. In conclusion, 1-mono-stearin identified from Aristolochia tagala Champ. was antimicrobial chemical against Sclerotinia sclerotiorum.
Keywords
antifungal active substance; aristolochia tagala champ.; sclerotinia sclerotiorum; 1-mono-stearin;
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Times Cited By KSCI : 7  (Citation Analysis)
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