The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Polyporus Herbal-acupuncture at KI10 on LPS-induced nephritis in rats

LPS로 유도된 흰쥐의 신장염에 대한 음곡(KI10) 저령약침의 효과

  • Lee, Jeong-Yun (Department of Meridian & Acupoint, College of korean Medicine, Daejeon University) ;
  • Jang, Seung-Hoon (Department of Meridian & Acupoint, College of korean Medicine, Daejeon University) ;
  • Jeon, Jong-Ik (Department of Meridian & Acupoint, College of korean Medicine, Daejeon University) ;
  • Yim, Yun-Kyoung (Department of Meridian & Acupoint, College of korean Medicine, Daejeon University)
  • 이정윤 (대전대학교 한의과대학 경락경혈학교실) ;
  • 장승훈 (대전대학교 한의과대학 경락경혈학교실) ;
  • 전종익 (대전대학교 한의과대학 경락경혈학교실) ;
  • 임윤경 (대전대학교 한의과대학 경락경혈학교실)
  • Received : 2015.06.08
  • Accepted : 2015.09.25
  • Published : 2015.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: The purpose of this study was to evaluate the effects of Polyporus Herbal-acupuncture(PO-HA) at KI10 (Umgok) on nephritis induced by lipopolysaccharide(LPS) in rats. Methods: Rats were allocated into normal, control, and 2 experimental groups. The rats in the control group were intra-peritoneally injected with LPS for nephritis induction. The rats in the groups of experiment 1 and experiment 2 were treated with Saline injection, and PO-HA, respectively at KI10 three times for a week and then intra-peritoneally injected with LPS. To evaluate the effects of PO-HA at KI10, WBC count in blood, creatine, tumor necrosis factor-alpha (TNF-${\alpha}$), cytokine-induced neutrophil chemoattractant 1(CINC-1) in serum, urinary volume, creatinine, total protein in urine, myeloperoxidase(MPO) in kidney were measured. Results: PO-HA at KI10 significantly suppressed the increase of WBC in blood, TNF-${\alpha}$, CINC-1 in serum, MPO in kidney of LPS-stimulated rats. PO-HA at KI10 significantly suppressed the increase creatinine, total protein in urine of LPS-stimulated rats. Conclusions: According to these results, it is postulated that PO-HA at KI10 has an anti-inflammatory and renal-protective effects on LPS-induced nephritis in rats. Therefore, it is suggested that PO-HA at KI10 may be an useful therapeutics for nephritis in clinical field after further researches.

Keywords

References

  1. Du HK. Oriental Kidney System Internal Medicine. Seoul:Institute of the oriental science. 1993:226, 228, 247, 238-47, 326, 334-6, 361-2, 454-9, 514-6.
  2. Du HK. Oriental Kidney System Internal Medicine. Seoul:Institute of the oriental science. 1993:225, 238-47, 256-7, 334-5, 359-401.
  3. Committee of Korean Textbook Publisher. Herbology. 6th ed. Seoul:Yeong Lim Sa. 1999: 304-5.
  4. Kim CM, Shin MK, Ahn DK, Lee KS. The encyclopedia of Oriental herbal medicine. Seoul:Jung Dam. 2004:3700.
  5. Sun Y, Yasukawa K. New anti-inflammatory ergostane-type ecdysteroids from the sclerotium of Polyporus umbellatus. Bioorganic & Medicinal Chemistry Letters. 2008;18:3417-20. https://doi.org/10.1016/j.bmcl.2008.04.008
  6. Ohsawa T, Yukawa M, Takao C, Murayama M, Bando H. Studies on constituents of fruit body of Polyporus umbellatus and their cytotoxic activity. Chem Pharm Bull. 1992;40 :143-7. https://doi.org/10.1248/cpb.40.143
  7. Oh YH, Lee UY, Lee MW, Shim MJ, Lee TS. Immnuo-modulatory and Antitumor Effect of Crude Polysaccharides Extracted from Sclerotium of Grifolia umbellata. The Korean Journal of Mycology. 2004;32:23-30. https://doi.org/10.4489/KJM.2004.32.1.023
  8. Zhang Y, Liu Y, Yan SC. Effect of Polyporus umbellatus polysaccahride on function of macrophages in the peritoneal cavities of mice with lesions. Cheih Ho Tsa Chih. 1991;11: 211-5.
  9. May L, Yun SC. Therapeutic investigation of Polyporus umbellatus polysaccahride on the children with pure immunity. J Traditional Chinese Medicine. 1990;3:168-9.
  10. Chang YF, Liu YY, Yun SC. Effect of Polyporus umbellatus polysaccahrides on macrophages of liver damaged mice. Intergrated J Chinese and Western Medicine. 1991;11: 225-6.
  11. Xiong LL. Therapeutic effect of combined therapy of Salvia miltiorrhizae and Polyporus umbellatus polysaccharide in the treatment of chronic hepatitis B. Cheih Ho Tsa Chih. 1993; 13:516-7.
  12. Korean Pharmacopuncture institute. Aqua-acupuncture and clinical use. Seoul:Korean Pharmacopuncture institute. 1997:1-5.
  13. Textbook Compilation Committee of Korean acupuncture & moxibustion medicine society. The acupuncture and Maxivustion (volume I). Seoul:JipMoonDang. 2001:531-63, 1457-60.
  14. Choi MS, Ko HK, Kim CH. A study on aqua-acupuncture. Journal of Korean acupuncture & moxibustion medicine society. 1990;7(1): 315-29.
  15. Meridian & Acupoint CIompilation Committee of Korean Oriental Medical Colleges. Detail of meridians & acupoints (volume II) : a guidebook for college students. Daejeon:JongRyeoNaMu. 2012:634-6.
  16. Kim KM, Lee H, Kang HJ, Lee YH, Yim YK. Anti-inflammatory Effect of Dianthi Semen Herbal-acupuncture at KI10 on nephritis in rats. Journal of Meridian & Acupoint. 2009; 26(1):66-77.
  17. Park BM, Hur B, Yim YK. Anti-inflammatory Effect of Plantaginis Semen Herbal-acupuncture at KI10 on LPS-induced nephritis in rats. Journal of Meridian & Acupoint. 2009;26(2): 127-43.
  18. Lee JH, Kim JH, Kim YI. Anti-inflammatory Effect of Benincasae Semen Herbal-acupuncture at KI10 on nephritis in rats. Journal of pharmacopuncture. 2010;13(2):51-65. https://doi.org/10.3831/KPI.2010.13.1.051
  19. Yoon KS, Kang JH, Lee H. Anti-inflammatory Effect on Desmodii Herba Pharmacopuncture at KI10 on Nephritis in Rats. Journal Of Korean Acupuncture & Moxibustion Medicine Society. 2011;28(3):43-54.
  20. Cho E, Kang JH, Lee H. Anti-inflammatory Effect of Akebiae Lignum Parmacopuncture at KI10 on LPS-induced Acute Nephritis in Rats. The Journal Of Korean Acupuncture & Moxibustion Medicine Society. 2012;29(3): 41-53.
  21. Medzhitov R, Preston-Hurlburt P, Janeway CA Jr. A human homologue of the Drosophila Toll protein signal activation of adaptive immunity. Nature. 1997;388(6640):394-7. https://doi.org/10.1038/41131
  22. Aderem A, Ulevitch RJ. Toll-like receptors in the induction of the innate immune response. Nature. 2000 ; 406(6797) : 782-7. https://doi.org/10.1038/35021228
  23. Hoshino K, Takeuchi O, Kawai T, Sanjo H, Ogawa T, Takeda Y, et al. Toll-like receptor 4(TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J immunol. 1999;162(7): 3749-52.
  24. Baumgartner J-D, Cohen J. Septic shock Pathogenesis. Lancet. 1991;338:732-6. https://doi.org/10.1016/0140-6736(91)91452-Z
  25. Ohmori Y, Hamilton TA. A macrophage LPS-inducible early gene encodes the murine homologue of IP-10. Biochem Biophys Res Commun. 1990;168:1261-7. https://doi.org/10.1016/0006-291X(90)91164-N
  26. Tannenbaum CS, Koemer TJ, Jansen MM, Hamilton TA. Characteriation of Lipopolysaccharide-induced macrophage gene expression. J immunol. 1988;140:3640-5.
  27. Abbas AK, Lichtman AH, Pillai S. Cellular and Molecular Immunology. 4th ed. trans. Kang JS et al. Seoul: PanMunSa. 2002:23-40, 240-3, 567-8.
  28. Harada A, Sekido N, Akahoshi T, Wada T, Mukaida N, Matsushima K. Essential involvement of interleukin-8(IL-8) in acute inflammation. Journal of Leukocyte Biology. 1994;56:559-64. https://doi.org/10.1002/jlb.56.5.559
  29. Matsushima K, Morishita K, Yoshimura T, Lavu S, Kobayashi Y, Lew W, et al. Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. J. Exp. Med. 1988;167:1883-93. https://doi.org/10.1084/jem.167.6.1883
  30. Strieter RM, Kunkel SL, Showell HJ, Remick DG, Phan SH, Ward PA, et al. Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-alpha, LPS, and IL-1 beta. Science. 1989;243:1467-9. https://doi.org/10.1126/science.2648570
  31. Sekido N, Mukaida N, Harada A, Nakanishi I, Watanabe Y, Matsushima K. Prevention of lung reperfusion injury in rabbits by a monoclonal antibody against interleukin-8. Nature. 1993;365:654-7. https://doi.org/10.1038/365654a0
  32. Krawisz JE, Sharon P, Stenson WF. Quantitive assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology. 1984;87:1344-55.
  33. Song KY, Ji JK, Ham UK. Core Pathology. Seoul:Korea Medical Book Publishing company. 1998:79-80, 573, 576-80.