Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
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Effects of direct current electric-field using ITO plate on breast cancer cell migration

  • Kim, Min Sung (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Lee, Mi Hee (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Kwon, Byeong-Ju (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Seo, Hyok Jin (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Koo, Min-Ah (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • You, Kyung Eun (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Kim, Dohyun (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Park, Jong-Chul (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
  • Received : 2014.06.19
  • Accepted : 2014.07.09
  • Published : 2014.09.01
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Abstract

Background: Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated. Results: Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was $-0.046{\pm}0.357$ under the 0 V/cm and was $0.273{\pm}0.231$ under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate. Conclusions: In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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