Browse > Article
http://dx.doi.org/10.1186/2055-7124-18-10

Effects of direct current electric-field using ITO plate on breast cancer cell migration  

Kim, Min Sung (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Lee, Mi Hee (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Kwon, Byeong-Ju (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Seo, Hyok Jin (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Koo, Min-Ah (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
You, Kyung Eun (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Kim, Dohyun (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Park, Jong-Chul (Cellbiocontrol Laboratory, Department of Medical Engineering, Yonsei University College of Medicine)
Publication Information
Biomaterials Research / v.18, no.3, 2014 , pp. 73-78 More about this Journal
Abstract
Background: Cell migration is an essential activity of the cells in various biological phenomena. The evidence that electrotaxis plays important roles in many physiological phenomena is accumulating. In electrotaxis, cells move with a directional tendency toward the anode or cathode under direct-current electric fields. Indium tin oxide, commonly referred to as ITO has high luminous transmittance, high infrared reflectance, good electrical conductivity, excellent substrate adherence, hardness and chemical inertness and hence, have been widely and intensively studied for many years. Because of these properties of ITO films, the electrotaxis using ITO plate was evaluated. Results: Under the 0 V/cm condition, MDA-MB-231 migrated randomly in all directions. When 1 V/cm of dc EF was applied, cells moved toward anode. The y forward migration index was $-0.046{\pm}0.357$ under the 0 V/cm and was $0.273{\pm}0.231$ under direct-current electric field of 1 V/cm. However, the migration speed of breast cancer cell was not affected by direct-current electric field using ITO plate. Conclusions: In this study, we designed a new electrotaxis system using an ITO coated glass and observed the migration of MDA-MB-231 on direct current electric-field of the ITO glass.
Keywords
Cell migration; Electrotaxis; ITO plate; Breast cancer cell; MDA-MB-231;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Li S, Guan JL, Chien S: Biochemistry and biomechanics of cell mortility. Annu Rev Biomed Eng 2005, 7:105-150.   DOI   ScienceOn
2 Lauffenburger DA, Horwitz AF: Cell migration: a physically integrated molecular process. Cell 1996, 84:359-369.   DOI   ScienceOn
3 Al-Bazzaz FJ, Gailey C: Ion transport by sheep distal airways in a miniature chamber. Am J Physiol 2001, 281:1028-1034.
4 Dortch-Carnes J, Van Scott MR, Fedan J: Changes in smooth muscle tone during osmotic challenge in relation to epithelial bioelectric events in guinea pig isolated trachea. J Pharmacol Exp Ther 1999, 289:911-917.
5 Wang Q, Horisberger JD, Maillard M, Brunner HR, Burnier M: Salt- and angiotensin II-dependent variations in amiloride-sensitive rectal potential difference in mice. Clin Exp Pharmacol Physiol 2000, 27:60-66.   DOI   ScienceOn
6 Szatkowski M, Mycielska M, Knowles R, Kho AL, Djamgoz MB: Electrophysiological recordings from the rat prostate gland in vitro: identified single-cell and transepithelial (lumen) potentials. BJU Int 2000, 86:1068-1075.
7 Sorensen E, Olesen J, Rask-Madsen J, Rask-Andersen H: The electrical potential difference and impedance between CSF and blood in unanesthetized man. Scand J Clin Lab Invest 1978, 38:203-207.   DOI
8 Sato MJ, Kuwayama H, Takayama ALK, Ueda M: Switching direction in electricsignal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling. Proc Natl Acad Sci U S A 2009, 106:6667-6672.   DOI   ScienceOn
9 Li J, Nandagopal S, Wu D, Lin F: Activated T lymphocytes migrate toward the cathode of DC electric fields in microfluidic devices. Lab Chip 2011, 11:1298-1304.   DOI   ScienceOn
10 Robinson KR: The responses of cells to electrical fields: A review. J Cell Biol 1985, 101:2023-2027.   DOI   ScienceOn
11 Nuccitelli R: A role for endogenous electric fields in wound healing. Curr Top Dev Biol 2003, 58:1-26.   DOI
12 Mycielska ME, Djamgoz MB: Cellular mechanisms of direct-current electric field effects: Galvanotaxis and metastatic disease. J Cell Sci 2004, 117:1631-1639.   DOI   ScienceOn
13 Nagatomo T, Maruta Y, Omoto O: Electrical and optical properties of vacuum-evaporated indium-tin oxide films with high electron mobility. Thin Solid Films 1990, 192:17-25.   DOI   ScienceOn
14 McCaig CD, Rajnicek AM, Song B, Zhao M: Controlling cell behavior electrically: Current views and future potential. Physiol Rev 2005, 85:943-978.   DOI   ScienceOn
15 Lin F: Lymphocyte electrotaxis in vitro and in vivo. J Immunol 2008, 181:2465-2471.   DOI
16 Weijtens CHL, Van Loon PAC: Influence of annealing on the optical properties of indium tin oxide. Thin Solid Films 1991, 196:1-10.   DOI   ScienceOn
17 Hamberg I, Granqvist CG: Evaporated Sn‐doped In2O3 films: Basic optical properties and applications to energy‐efficient windows. J Appl Phys 1986, 60:123-160.   DOI
18 Nishio K, Sei TI, Tsuchiya T: Preparation and electrical properties of ITO thin films by dip-coating process. J Mat Sci 1996, 31:1761-1766.   DOI
19 Djaoued Y, Phong VH, Badilescu S, Ashrit PV, Girouard FE, Truong V: Sol-gel-prepared ITO films for electrochromic systems. Thin Solid Films 1997, 293:108-112.   DOI   ScienceOn
20 Mattox DM: Sol-gel derived, air-baked indium and tin oxide films. Thin Solid Films 1991, 204:25-32.   DOI   ScienceOn
21 Faughnan BW, Crandall RS: Electrochromic displays based on $WO_3$. Topics Applied Physics 1980, 40:181-211.   DOI
22 Meng L, Li C, Zhong G: The influence of the concentration of $Er^{3+}$ ions on the characteristics of AC-electroluminescence in ZnS:$ErF_3$ thin films. J Lumin 1987, 39:11-17.   DOI   ScienceOn
23 Luff BJ, Wilkinson JS, Perrone G: Indium tin oxide overlayered waveguides for sensor applications. Appl Opt 1997, 36:7066-7072.   DOI
24 Bellingham JR, Mackenzie AP, Phillips WA: Precise measurements of oxygen content: Oxygen vacancies in transparent conducting indium oxide films. Appl Phys Lett 1991, 58:2506-2508.   DOI
25 Valentini A, Quaranta F, Penza ME, Rizzi FR: The stability of zinc oxide electrodes fabricated by dual ion beam sputtering. J Appl Phys 1993, 73:1043-1045.   DOI
26 Ozasa K, Ye T, Aoyagi Y: Deposition of thin indium oxide film and its application to selective epitaxy for in situ processing. Thin Solid Film 1994, 246:58-64.   DOI   ScienceOn
27 Tamisier L, Caprani A: Electrochemical study of the $Fe(CN){^{4-}}_6/Fe(CN){^{3-}}_6$ couple at the ITO/NaCl interface. Electrochim Acta 1987, 32:1365-1369.   DOI   ScienceOn
28 Alam MJ, Cameron DC: Optical and electrical properties of transparent conductive ITO thin films deposited by sol-gel process. Thin Solid Films 2000, 00:455-459.
29 Visser W, Scheffers WA, Vegte WHB, Dijken JP: Oxygen requirements of yeasts. Appl Environ Microbiol 1990, 56:3785-3792.
30 Reinheimer JA, Demkow MR: Comparison of rapid tests for assessing UHT milk sterility. J Dairy Res 1990, 57:239-243.   DOI
31 White MJ, DiCaprio MJ, Greenberg DA: Assessment of neuronal viability with Alamar blue in cortical and granule cell cultures. J Neurosci Methods 1996, 70:195-200.   DOI   ScienceOn
32 Yang HY, Charles RP, Hummler E, Baines DL, Isseroff RR: The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes. J Cell Sci 2013, 126:1942-1951.   DOI   ScienceOn