Identification of Afzelin and Quercitrin from Pinus koraiensis and Their Contents in Genus Pinus Using HPLC/UV Analysis

  • Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Ryu, Min Ju (Department of Food and Nutrition, Duksung Women's University) ;
  • Cho, Sunghun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Chung, Ha Sook (Department of Food and Nutrition, Duksung Women's University) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2014.04.18
  • Accepted : 2014.07.10
  • Published : 2014.09.30

Abstract

Phytochemical constituents were isolated from Pinus koraiensis needles by open column chromatography. The structures isolated from the ethyl acetate fraction of P. koraiensis needles were elucidated as afzelin (1) and quercitrin (2) on the basis of spectroscopic analyses including $^1H$-NMR and MS. The amounts of afzelin (1) and quercitrin (2) in genus Pinus were determined by HPLC/UV analysis. Afzelin (1) and quercitrin (2) contents were highest in P. thunbergii needles. Because P. thunbergii needles contain high amounts of afzelin (1) and quercitrin (2), they have promising potential as new additives to natural medicinal products, health supplements, and beverages.

Keywords

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