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Characteristics of Watermelon Mosaic Virus Transmission Occurring in Korean Ginseng

인삼에서 발생하는 수박모자이크바이러스의 감염 특성

  • Choi, Seung-Kook (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Cho, In-Sook (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Chung, Bong-Nam (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Mi-Kyeong (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jung, Won-Kwon (Punggi Ginseng Experiment Station, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Choi, Gug-Seoun (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 최승국 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 정봉남 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 김미경 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 정원권 (경상북도농업기술원 풍기인삼시험장) ;
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과)
  • Received : 2014.02.04
  • Accepted : 2014.07.22
  • Published : 2014.09.30

Abstract

Korean ginseng (Panax ginseng) is the most popular herb for medical purpose in Korea. Recently, viral diseases from Korean ginseng showing various degrees of severe mottling, variegation and mosaic symptoms have caused quantity losses of Korean ginseng in a large number of farms. Watermelon mosaic virus (named WMV-gin) was identified as a causal agent for the disease of Korean ginseng. Interestingly, WMV-gin failed to infect both Korean ginseng plant and susceptible host species including cucurbitaceous plants by mechanical inoculation. However, WMV-gin could successfully infect Korean ginseng by transmission of two aphid species (Myzus persicae and Aphis gossypii). It is likely that transmission of WMV-gin was done by both the aphid species during feeding behavior of the two aphid species on Korean ginseng, though the aphids dislike feeding in Korea ginseng. Similarly, a strain of WMV (WMV-wm) isolated from watermelon was transmitted successfully to Korean ginseng plant by the two aphid species, but not by mechanical inoculations. Transmission assays using M. persicae and A. gossypii clearly showed both WMV-gin and WMV-wm were not transmitted from infected Korean ginseng plant to cucurbit species that are good host species for WMV. These results suggest WMV disease occurring in Korean ginseng plant can be controlled by ecological approaches.

인삼은 우리나라에서 가장 대중화된 한약 재료로 사용되고 있다. 최근 인삼 잎에 얼룩덜룩하거나 모자이크 증상이 나타나는 바이러스병으로 인삼을 재배하는 많은 농가들에서 수량 손실을 초래하고 있다. 수박모자이크바이러스(WMV-gin로 명명)가 인삼 바이러스병의 원인으로 동정되었다. 흥미롭게도 인공적 즙액 접종에 의해서, 인삼에서 분리한 WMV-gin은 건전 인삼 식물체와 박과작물을 포함한 알려진 WMV 기주 식물 종들을 감염시킬 수 없었다. 그러나 WMV-gin은 2종의 진딧물들(복숭아혹진딧물과 목화진딧물)의 전반에 의해서 인삼을 성공적으로 감염시킬 수 있었다. 비록 2종의 진딧물들이 인삼에서 먹이를 얻는 것을 기피하지만, 이것은 진딧물들이 먹이 탐색 과정동안 인삼에 WMV-gin을 매개하는 것으로 사료된다. 유사하게 수박에서 분리한 WMV-wm 계통은 2종의 진딧물들에 의하여 인삼으로 매개되지만 인위적 즙액 접종으로 인삼을 감염시키지 못하였다. 복숭아혹진딧물과 목화진딧물을 이용한 전반 실험들에 의해서 WMV-gin과 WMV-wm 계통들 모두 인삼에서 WMV의 증식 기주 식물들인 박과 식물들로 전반되지 못함이 밝혀졌다. 이런 결과들은 인삼에서 발생하는 WMV병이 생태학적 접근법에 의해서 방제될 수 있음을 제시해 준다.

Keywords

References

  1. Al-Musa, A. and Mansour, A. 1982. Some properties of a watermelon mosaic virus in Jordan. Plant Dis. 66: 330-331. https://doi.org/10.1094/PD-66-330
  2. Castle, S. J., Perring, T. M., Garrar, C. A. and Kishaba, A. N. 1992. Field and laboratory transmission of watermelon mosaic virus 2 and zucchini yellow mosaic virus by various aphid species. Phytopathology 82: 235-240. https://doi.org/10.1094/Phyto-82-235
  3. Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U. and Ball, L. A. (eds.) 2005. Virus Taxonomy, Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier Academic Press, San Diego, CA, USA.
  4. Jung, W. K., Kim, M. K., Lee, J. H., Park, C. Y., Kim, B. H., Park, E. H., Lee, M. A., Kim, M. K., Choi, H. S., Lee, J. S., Kim, J. S., Choi, J. K., Kwon, T. R., Lee, K. W. and Lee, S. H. 2013. Novel pathogenic strain of Watermelon mosaic virus occurred on Insam (Panax ginseng). Res. Plant Dis. 19: 331-337. (In Korean) https://doi.org/10.5423/RPD.2013.19.4.331
  5. Lee, J. T. 1982. Studies on virus diseases of medicinal plants in Korea. Res. Rev. Kyoungpook Univ. 34: 505-515.
  6. Mishchenko, L. T., Koreneva, A. A., Molchanets, O. V. and Boyko, A. L. 2009. Detection of infection agents in medicinal plant in conditions of Ukraine. Mikrobiol. Z. 71: 55-61.
  7. Kim, H. G. and Park, S. J. 2011. Virus disease incidences and transmission ecology of oriental melons in Seongju Area. Res. Plant Dis. 17: 342-350. (In Korean) https://doi.org/10.5423/RPD.2011.17.3.342
  8. Purcifull, D., Heibert, E. and Edwarson, J. 1984. Watermelon mosaic virus 2. No. 293. CMI/AAB Description, England.
  9. Radcliffe, E. B. and Ragsdale, D. W. 2002. Aphid-transmitted potato viruses: The importance of understanding vector biology. American J. Potato Res. 79: 353-386. https://doi.org/10.1007/BF02870173
  10. Shukla, D. D., Ward, C. W. and Brunt, A. A. 1994. The Potyviridae. CAB International, Wallingford, UK.
  11. Tao, Z., Yan. Z., Song, L., Li, H. and Fan, Z. 2008. Molecular identification of viruses that infect Panax notoginseng in China. Phytopathology 98: S155.
  12. The Korean Society of Plant Pathology. 2009. List of plant diseases in Korea, 5th ed. (In Korean)
  13. Walters, S. A. 2003. Suppression of watermelon mosaic virus in summer squash with plastic mulches and rowcovers. HortiTechnology 13: 352-357.
  14. Zhang, Y. J., Yin, J., Li, G. F., Li, M. F., Huang, X., Chen, H. J., Zhao, W. J. and Zhus, S. F. 2010. Oligonucleotide microarry with a minimal number of probes for the detection and identification of thirteen genera of plant viruses. J. Virol. Methods. 167: 53-60. https://doi.org/10.1016/j.jviromet.2010.03.010
  15. Zitter, T. A., Hopkins, D. L. and Thomas, C. E. 1996. Compendium of cucurbit diseases. APS Press, St. Paul, USA.