Journal of Magnetics

The Korean Magnetics Society (한국자기학회)
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Effects of Magnetized Medium on In Vitro Maturation of Porcine Cumulus Cell-Oocyte Complexes

  • Kim, Yun-Jung (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Jung, Soo-Jung (Financial Accounting, Seoul National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • Received : 2014.03.04
  • Accepted : 2014.07.03
  • Published : 2014.09.30
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Abstract

The objective of this study was to study the effect of magnetized water on porcine cumulus cell-oocyte complexes (COCs). Oocytes obtained from female pig were cultured in a medium magnetized at 0, 2000, 4000, and 6000 Gauss (G) for 5 minutes using the neodymium magnet. Subsequently, intracellular hydrogen peroxide ($H_2O_2$) concentration, glutathione (GSH) activity, oocyte membrane integrity, anti-apoptosis factor Bcl-xL expression, and nuclear maturation were analyzed. The intracellular $H_2O_2$ levels in COCs cultured for 44 hours were not significantly different among the variously magnetized samples. However, GSH activity were significantly higher in the magnetized samples compared to the 0 G sample. The Bcl-xL mRNA expression in COCs cultured for 44 hours was higher in the 4000 G sample than other treatment groups. Membrane damage in COCs cultured for 22 and 44 hours was significantly lower in 4000 G group than control group. On the other hand, nuclear stages as maturation indicator significantly increased in 2000, 4000, and 6000 G groups compared to 0 G group. These results indicate that incubation of porcine oocytes and cumulus cells in magnetized medium improves intracellular GSH levels, membrane integrity and nuclear maturation, and inhibits apoptosis in vitro.

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