Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital

  • Kim, Semi (Department of Laboratory Medicine, College of Medicine, Chungnam National University) ;
  • Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University) ;
  • Cho, Hye Hyun (Department of Biomedical Laboratory Science, Jeonju Kijeon College) ;
  • Kwon, Kye Chul (Department of Laboratory Medicine, College of Medicine, Chungnam National University) ;
  • Koo, Sun Hoe (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
  • Received : 2013.06.13
  • Accepted : 2014.03.13
  • Published : 2014.06.28
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Abstract

This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and 24 (77.4%) K. pneumoniae isolates containing $bla_{CTX-M}$, $bla_{CTX-M-14}$ and $bla_{CTX-M-15}$ were detected in 23 and 19 E. coli isolates, respectively, and in 7 and 17 K. pneumoniae isolates, respectively. CTX-M producers of urinary E. coli and K. pneumoniae were resistant to multiple antibiotics and contained other antimicrobial resistance genes. CTX-M-15 producers contained more antimicrobial resistance genes than did CTX-M-14 producers.

Keywords

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