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http://dx.doi.org/10.4014/jmb.1306.06036

Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital  

Kim, Semi (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Sung, Ji Youn (Department of Biomedical Laboratory Science, Far East University)
Cho, Hye Hyun (Department of Biomedical Laboratory Science, Jeonju Kijeon College)
Kwon, Kye Chul (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Koo, Sun Hoe (Department of Laboratory Medicine, College of Medicine, Chungnam National University)
Publication Information
Journal of Microbiology and Biotechnology / v.24, no.6, 2014 , pp. 765-770 More about this Journal
Abstract
This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and 24 (77.4%) K. pneumoniae isolates containing $bla_{CTX-M}$, $bla_{CTX-M-14}$ and $bla_{CTX-M-15}$ were detected in 23 and 19 E. coli isolates, respectively, and in 7 and 17 K. pneumoniae isolates, respectively. CTX-M producers of urinary E. coli and K. pneumoniae were resistant to multiple antibiotics and contained other antimicrobial resistance genes. CTX-M-15 producers contained more antimicrobial resistance genes than did CTX-M-14 producers.
Keywords
CTX-M-14; CTX-M-15; extended-spectrum ${\beta}$-lactamase (ESBL); E. coli; K. pneumoniae;
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