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http://dx.doi.org/10.6116/kjh.2014.29.4.83.

Anti-inflammatory Effect of Houttuyniae Herba Water Extract on LPS-induced RAW 264.7 Mouse Macrophages  

Hwang, In Seung (Department of Pathology, College of Korean Medicine, Gachon University)
Kim, Young Jin (Department of Pathology, College of Korean Medicine, Gachon University)
Park, Yun Soo (Department of Pathology, College of Korean Medicine, Gachon University)
Kim, Hyun Ju (Department of Pathology, College of Korean Medicine, Gachon University)
Kim, Do Hoon (Department of Oriental Medical Classics & History, College of Korean Medicine, Gachon University)
Park, Wan Su (Department of Pathology, College of Korean Medicine, Gachon University)
Publication Information
The Korea Journal of Herbology / v.29, no.4, 2014 , pp. 83-89 More about this Journal
Abstract
Objectives : The purpose of this study was to investigate effects of Houttuyniae Herba water extract (HC) on calcium release and production of various inflammatory mediators such as nitric oxide (NO), interferon-inducible protein (IP)-10, platelet derived growth factor (PDGF)-BB, keratinocyte-derived chemokine (KC), vascular endothelial growth factor (VEGF), interleukin (IL)-4, and IL-5 in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages. Methods : NO production was measured by Griess reagent assay. Intracellular calcium level was measured with Fluo-4 assay. Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. Results : HC significantly decreased NO production for 24 hrs incubation at the concentrations of 10, 25, 50, 100, and $200{\mu}/mL$ in LPS-induced RAW 264.7 (P < 0.05). HC significantly decreased production of IP-10, KC, VEGF, and PDGF-BB for 24 hrs incubation at the concentrations of 50, 100, and $200{\mu}/mL$ in LPS-induced RAW 264.7 (P < 0.05). HC also significantly decreased intracellular calcium release for 24 hrs incubation at the concentrations of 25, 50, 100, and $200{\mu}/mL$ in LPS-induced RAW 264.7 (P < 0.05). But HC did not show any significant effect on production of IL-4 and IL-5 in LPS-induced RAW 264.7. Conclusions : The results suggested that HC has anti-inflammatory property related with its inhibition on the production of NO, IP-10, KC, VEGF, and PDGF-BB in LPS-induced macrophages via calcium pathway.
Keywords
Houttuyniae Herba; Macrophage; Inflammation; Cytokine; Nitric oxide; Calcium;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Jeong BC, Kim DC, Baek SH, Kim EH. The effect of Houttuynia cordata thunberg on the inhibition of growth of leiomyomas and apoptosis. J Kor Obstet Gynecol. 2007 ; 20(3) : 1-12.   과학기술학회마을
2 Barnes PJ, Chung KF, Page CP. Inflammatory mediators of asthma: an update. Pharmacol Rev. 1998 ; 50 : 515-96.
3 Long MJ, Tucci MA, Benghuzzi HA. Human gingival fibroblast cell response to gox/cat. Biomed Sci Instrum. 2012 ; 48 : 268-74.
4 Zhang Y, Xu CB, Cardell LO. Long-term exposure to IL-1beta enhances Toll-IL-1 receptor-mediated inflammatory signaling in murine airway hyperresponsiveness. Eur Cytokine Netw. 2009 ; 20 : 148-56.
5 Yoon SB, Lee YJ, Park SK, Kim HC, Bae H, Kim HM, Ko SG, Choi HY, Oh MS, Park W. Anti-inflammatory effects of Scutellaria baicalensis water extract on LPS-activated RAW 264.7 macrophages. J Ethnopharmacol. 2009 ; 125 : 286-90.   DOI   ScienceOn
6 Kim HS. Isolation from Houttuynia Cordata and their anti-inflammatory activity. Graduate School of Joongbu University. 2010 : 75-81.
7 Lee JY, Lee YJ, Park WS. Anti-inflammatory Effects of Fermented Houttuyniae Herba Water Extract on LPS-induced Mouse Macrophage. Kor J Herbology. 2010 ; 25 : 27-34.   과학기술학회마을
8 Park WS. Effect of White Ginseng-Ejung-tang Water Extract on Cytokine Production in LPS-induced RAW 264.7 Mouse Macrophages. Kor J Ori Med Physiol Pathol. 2012 ; 27(6) : 738-44.   과학기술학회마을
9 An JH(as editor). Joongyakhak. Beijing : Inminwisangchulpansa. 1995 : 183-5.
10 Choi SD(as editor). Joongyakdaejeon. Habei : Heukroynggangguahakkisuchulpansa. 1989 : 301-2.
11 Shin MK. Imsangbonchohak. Seoul : Younglimsa. 1997 : 574-5.
12 Park WS. Effect of Sacchromyces cerevisiae-Fermented Artemisiae Argi Folium on Nitric oxide Production of Macrophage Treated with Toxicants. Kor J Ori Med Physiol Pathol. 2009 ; 23(4) : 883-7.   과학기술학회마을
13 Park WS. The Effect of Bacillus-Fermented Scutellariae Radix Acupuncture Solution on Interleukin Production in Mouse Macrophage Stimulated by Lipopolysaccharide. Kor J Acu. 2010 ; 27(2) : 95-105.   과학기술학회마을
14 Park WS. Inhibitory Effect of Gallic acid on Production of Chemokine and Growth Factor in Mouse Macrophage Stimulated by Lipopolysaccharide. Kor J Ori Med Physiol Pathol. 2010 ; 24(4) : 586-91.   과학기술학회마을
15 Lee JY, Park W. Anti-Inflammatory Effect of Myristicin on RAW 264.7 Macrophages Stimulated with Polyinosinic-Polycytidylic Acid. Molecules. 2011 ; 16(8) : 7132-42.   DOI
16 Lee JY, Park W, Yi DK. Immunostimulatory effects of gold nanorod and silica-coated gold nanorod on RAW 264.7 mouse macrophages. Toxicol Lett. 2012 ; 209(1) : 51-7.   DOI   ScienceOn
17 Jeong HR, Kwak JH, Kim JH, Choi GN, Jeong CH, Heo HJ. Antioxidant and Neuronal Cell Protective Effects of an Extract of Houttuynia cordata Thunb (a Culinary Herb). Korean J Food Preserv. 2010 ; 17(5) : 720-6.   과학기술학회마을
18 Dae SM. Junggukyakjaehak(ha). Daibei : Upsuoguk. 1981 : 1058-9.
19 Chunkukhaniguadaehak Gongdonggyoujaepyouchanwewonhoi. Bonchohak. Seoul : Younglimsa. 1991 : 270-1.
20 Na G, Sa MJ. Chungjipmyoungwibyulrokjeonmoon (Vol. 1). Daijoong : Joonggukwiyakhakwonyoungu. 1976 : 297.
21 Ahn DG. Wonsaekhangukbonchodogam. Seoul : Kyohaksa. 1998 : 98.
22 Jeong BS, Shin MK. Dohaehyangyak(saengyak) daesajeun(sikmulpyun). Seoul : Younglimsa. 1990 : 812-3.
23 Sanghaijoonguihakwon. Joongchoyakhak. Shanghai : Sangmuinseguan. 1975 : 150-1.
24 Ryu CA, Paeng MP. Hangamjoongchoyakdaesajeun. Hobuk : Jobukguahakkisulchulpansa. 1994 : 665-59.
25 Lee JY, Kim YJ, Park WS. Effects of White Ginseng-Ejung-tang Acupuncture Solution on Nitric Oxide and Hydrogen Peroxide Production in LPS-induced Mouse Macrophages. Kor J Acu. 2011 ; 28(1) : 61-9.   과학기술학회마을
26 Gansoshineihakwon. Chinese Meteria Medica. Hongkong : Shanghaigisulchulpansa. 1978 : 1439-41.