Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Rapid detection of the hepatitis a virus from fresh lettuce using immunomagnetic separation and quantum dots assay

IMS-QD assay를 활용한 상추에서 간염 A형 바이러스의 신속순수분리 및 형광 검출법 연구

  • 이희민 (전남대학교 식품공학과.기능성식품연구센터) ;
  • 권요셉 (한국기초과학지원연구원) ;
  • 최종순 (한국기초과학지원연구원) ;
  • 원용관 (전남대학교 전자컴퓨터공학부) ;
  • 김은선 (광주광역시보건환경연구원) ;
  • 정재근 (광주광역시보건환경연구원) ;
  • 김민지 (광주광역시보건환경연구원) ;
  • 김두운 (전남대학교 식품공학과.기능성식품연구센터)
  • Received : 2013.09.29
  • Accepted : 2014.01.02
  • Published : 2014.04.30
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Abstract

Hepatitis A virus (HAV) infection leads to acute liver failure and death through the intake of contaminated food. The polymerase chain reaction (PCR) has been used to detect HAV in food samples. HAV detection takes a long time, however, due to the virus concentration step required before PCR assay. In this study, a rapid method of detecting the HAVs present in lettuce using immunomagnetic separation combined with quantum dots (IMS-QDs) assay was developed. The detection limit of IMS-QDs for HAV was 10 $TCID_{50}/mL$, similar to the result that was obtained using RT-PCR combined with PEG or IMS. The application of IMS-QDs assay completed the viral detection within one hour, but this was not possible using PEG combined with RT-PCR. In conclusion, IMS-QDs assay is a rapid and efficient method for detecting HAV at a low concentration in agricultural products.

식중독 유발 바이러스인 HAV는 제 1군 감염병원으로 규정되면서 감염 시 원인식품을 빠르게 분석하게 되었으며 그로 인해 정확하면서 빠른 검출기술을 요구하게 되었다. 본 연구에서는 HAV에 오염된 상추에서 신속하게 바이러스를 검사하기 위해서 IMS를 통하여 신속하게 HAV를 순수 분리 및 농축하였고, 형광물질인 quantum dot을 활용하여 형광검출을 실시하였다. 또한 일반적으로 바이러스 농축에 사용되는 PEG 농축방법과 비교하였을 때 검출능은 유사한 결과를 얻었으나, 농축시간 면에서는 IMS를 통한 방법이 효과적이었다. 또한 IMS 방법으로 확보된 항원을 Quantum dot을 활용하여 10분 이내에 바이러스를 검출할 수 있었다. 본 연구에서 제시된 검출기반은 식품 유통 과정 중 다양한 식중독 바이러스로부터 소비자를 보호 할 수 있는 검사방법으로 활용될 수 있다고 기대된다.

Keywords

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